Abstract
The nucleotide sequence of the hydHG operon, comprised of chromosomal genes that regulate labile hydrogenase activity in Salmonella typhimurium, was compared with the reported hydHG sequence of Escherichia coli. Nucleotide sequence analysis of a 4.8 kb EcoRI fragment of Salmonella chromosomal DNA revealed that one of the open reading frames (ORF) encoded a protein of 441 amino acid residues. This large ORF was identified on a 2.7 kb EcoRI/HindIII fragment and coded for the complete hydG gene. The carboxy-terminus (626 bp) of the hydH gene also was present immediately upstream of hydG. Expression of the Salmonella hydG gene in a T7 promoter/ polymerase system revealed the presence of a unique 45 kDa protein band. The incomplete hydH gene was not expressed. It is proposed that the labile hydrogenase activity in S. typhimurium may be regulated by the multiple component system.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 115-118 |
| Number of pages | 4 |
| Journal | BBA - Gene Structure and Expression |
| Volume | 1129 |
| Issue number | 1 |
| DOIs | |
| State | Published - Dec 2 1991 |
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Keywords
- DNA sequencing
- hydG gene
- hydH gene
- T7 polymerase/promoter system
ASJC Scopus subject areas
- Biochemistry
- Biophysics
- Genetics
- Structural Biology
Cite this
Cloning and sequence analysis of hydrogenase regulatory genes (hydHG) from Salmonella typhimurium. / Chopra, Ashok; Peterson, Johnny; Prasad, Rajendra.
In: BBA - Gene Structure and Expression, Vol. 1129, No. 1, 02.12.1991, p. 115-118.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Cloning and sequence analysis of hydrogenase regulatory genes (hydHG) from Salmonella typhimurium
AU - Chopra, Ashok
AU - Peterson, Johnny
AU - Prasad, Rajendra
PY - 1991/12/2
Y1 - 1991/12/2
N2 - The nucleotide sequence of the hydHG operon, comprised of chromosomal genes that regulate labile hydrogenase activity in Salmonella typhimurium, was compared with the reported hydHG sequence of Escherichia coli. Nucleotide sequence analysis of a 4.8 kb EcoRI fragment of Salmonella chromosomal DNA revealed that one of the open reading frames (ORF) encoded a protein of 441 amino acid residues. This large ORF was identified on a 2.7 kb EcoRI/HindIII fragment and coded for the complete hydG gene. The carboxy-terminus (626 bp) of the hydH gene also was present immediately upstream of hydG. Expression of the Salmonella hydG gene in a T7 promoter/ polymerase system revealed the presence of a unique 45 kDa protein band. The incomplete hydH gene was not expressed. It is proposed that the labile hydrogenase activity in S. typhimurium may be regulated by the multiple component system.
AB - The nucleotide sequence of the hydHG operon, comprised of chromosomal genes that regulate labile hydrogenase activity in Salmonella typhimurium, was compared with the reported hydHG sequence of Escherichia coli. Nucleotide sequence analysis of a 4.8 kb EcoRI fragment of Salmonella chromosomal DNA revealed that one of the open reading frames (ORF) encoded a protein of 441 amino acid residues. This large ORF was identified on a 2.7 kb EcoRI/HindIII fragment and coded for the complete hydG gene. The carboxy-terminus (626 bp) of the hydH gene also was present immediately upstream of hydG. Expression of the Salmonella hydG gene in a T7 promoter/ polymerase system revealed the presence of a unique 45 kDa protein band. The incomplete hydH gene was not expressed. It is proposed that the labile hydrogenase activity in S. typhimurium may be regulated by the multiple component system.
KW - DNA sequencing
KW - hydG gene
KW - hydH gene
KW - T7 polymerase/promoter system
UR - http://www.scopus.com/inward/record.url?scp=0026066284&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0026066284&partnerID=8YFLogxK
U2 - 10.1016/0167-4781(91)90224-A
DO - 10.1016/0167-4781(91)90224-A
M3 - Article
VL - 1129
SP - 115
EP - 118
JO - Biochimica et Biophysica Acta - Gene Structure and Expression
JF - Biochimica et Biophysica Acta - Gene Structure and Expression
SN - 0167-4781
IS - 1
ER -