Cloning and sequence analysis of hydrogenase regulatory genes (hydHG) from Salmonella typhimurium

Ashok Kumar Chopra, Johnny Wayne Peterson, Rajendra Prasad

Research output: Contribution to journalArticlepeer-review

14 Scopus citations

Abstract

The nucleotide sequence of the hydHG operon, comprised of chromosomal genes that regulate labile hydrogenase activity in Salmonella typhimurium, was compared with the reported hydHG sequence of Escherichia coli. Nucleotide sequence analysis of a 4.8 kb EcoRI fragment of Salmonella chromosomal DNA revealed that one of the open reading frames (ORF) encoded a protein of 441 amino acid residues. This large ORF was identified on a 2.7 kb EcoRI/HindIII fragment and coded for the complete hydG gene. The carboxy-terminus (626 bp) of the hydH gene also was present immediately upstream of hydG. Expression of the Salmonella hydG gene in a T7 promoter/ polymerase system revealed the presence of a unique 45 kDa protein band. The incomplete hydH gene was not expressed. It is proposed that the labile hydrogenase activity in S. typhimurium may be regulated by the multiple component system.

Original languageEnglish (US)
Pages (from-to)115-118
Number of pages4
JournalBBA - Gene Structure and Expression
Volume1129
Issue number1
DOIs
StatePublished - Dec 2 1991

Keywords

  • DNA sequencing
  • T7 polymerase/promoter system
  • hydG gene
  • hydH gene

ASJC Scopus subject areas

  • Structural Biology
  • Biophysics
  • Biochemistry
  • Genetics

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