Cloning, characterization, and expression of the rat relaxin gene

Melvyn S. Soloff, Sangwan Gal, Sarasija Hoare, Carl A. Peters, Mary Hunzicker-Dunn, Garland D. Anderson, Thomas Wood

Research output: Contribution to journalArticle

18 Citations (Scopus)

Abstract

Relaxin, a hormone in the insulin superfamily, is synthesized by the corpus luteum of the rat ovary. Expression of relaxin precursor mRNA in rats is sharply induced after day 10 of pregnancy and plateaus on days 15 to 20 (parturition occurs on day 23). In an effort to understand this induction, we cloned the gene and carried out promoter analyses by transient transfection and chromatin immunoprecipitation methods. The single gene is 2.9 kilobases and is composed of two exons and one intron. There are alternative splice acceptor sites, 3 base pairs apart, which account for the inclusion of an extra codon in about 10% of the transcripts. The induction of transcription by day 15 was observed by the binding of polymerase II and histone H3 acetylation at the promoter region. There is a functional STAT binding site, about 3.8 kb upstream from the transcriptional start site, that is occupied by STAT3 on day 6 of pregnancy, when relaxin expression is minimal; on day 15, when expression is maximal, STAT3 is replaced by STAT5a. These data are consistent with STAT5 playing a role in the induction of relaxin expression.

Original languageEnglish
Pages (from-to)149-155
Number of pages7
JournalGene
Volume323
Issue number1-2
DOIs
StatePublished - Dec 24 2003

Fingerprint

Relaxin
Organism Cloning
RNA Splice Sites
Genes
Pregnancy
Chromatin Immunoprecipitation
Corpus Luteum
RNA Precursors
Acetylation
Genetic Promoter Regions
Codon
Base Pairing
Histones
Introns
Transfection
Ovary
Exons
Binding Sites
Parturition
Hormones

Keywords

  • Chromatin immunoprecipitation
  • Prolactin
  • STAT3
  • STAT5a

ASJC Scopus subject areas

  • Genetics

Cite this

Soloff, M. S., Gal, S., Hoare, S., Peters, C. A., Hunzicker-Dunn, M., Anderson, G. D., & Wood, T. (2003). Cloning, characterization, and expression of the rat relaxin gene. Gene, 323(1-2), 149-155. https://doi.org/10.1016/j.gene.2003.09.015

Cloning, characterization, and expression of the rat relaxin gene. / Soloff, Melvyn S.; Gal, Sangwan; Hoare, Sarasija; Peters, Carl A.; Hunzicker-Dunn, Mary; Anderson, Garland D.; Wood, Thomas.

In: Gene, Vol. 323, No. 1-2, 24.12.2003, p. 149-155.

Research output: Contribution to journalArticle

Soloff, MS, Gal, S, Hoare, S, Peters, CA, Hunzicker-Dunn, M, Anderson, GD & Wood, T 2003, 'Cloning, characterization, and expression of the rat relaxin gene', Gene, vol. 323, no. 1-2, pp. 149-155. https://doi.org/10.1016/j.gene.2003.09.015
Soloff MS, Gal S, Hoare S, Peters CA, Hunzicker-Dunn M, Anderson GD et al. Cloning, characterization, and expression of the rat relaxin gene. Gene. 2003 Dec 24;323(1-2):149-155. https://doi.org/10.1016/j.gene.2003.09.015
Soloff, Melvyn S. ; Gal, Sangwan ; Hoare, Sarasija ; Peters, Carl A. ; Hunzicker-Dunn, Mary ; Anderson, Garland D. ; Wood, Thomas. / Cloning, characterization, and expression of the rat relaxin gene. In: Gene. 2003 ; Vol. 323, No. 1-2. pp. 149-155.
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