Co-localization of glyceraldehyde-3-phosphate dehydrogenase with ferredoxin-NADP reductase in pea leaf chloroplasts

Surendra S. Negi, Andrew A. Carol, Shivangi Pandya, Werner Braun, Louise E. Anderson

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

In immunogold double-labeling of pea leaf thin sections with antibodies raised against ferredoxin-NADP reductase (EC 1.18.1.2, FNR) and antibodies directed against the A or B subunits of the NADP-linked glyceraldehyde-3-P dehydrogenase (GAPD) (EC 1.2.1.13), many small and large gold particles were found together over the chloroplasts. Nearest neighbor analysis of the distribution of the gold particles indicates that FNR and the NADP-linked GAPD are co-localized, in situ. This suggests that FNR might carry FADH2 or NADPH from the thylakoid membrane to GAPD, or that ferredoxin might carry electrons to FNR co-localized with GAPD in the stroma. Crystal structures of the spinach enzymes are available. When they are docked computationally, the proteins appear, as modeled, to be able to form at least two different complexes. One involves a single GAPD monomer and an FNR monomer (or dimer). The amino acid residues located at the putative interface are highly conserved on the chloroplastic forms of both enzymes. The other potential complex involves the GAPD A2B2 tetramer and an FNR monomer (or dimer). The interface residues are conserved in this model as well. Ferredoxin is able to interact with FNR in either complex.

Original languageEnglish (US)
Pages (from-to)18-30
Number of pages13
JournalJournal of Structural Biology
Volume161
Issue number1
DOIs
StatePublished - Jan 2008

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Ferredoxin-NADP Reductase
Glyceraldehyde
Glyceraldehyde-3-Phosphate Dehydrogenases
Peas
Chloroplasts
Oxidoreductases
NADP
Ferredoxins
Glyceraldehyde-3-Phosphate Dehydrogenase (NADP+)(Phosphorylating)
Gold
Thylakoids
Spinacia oleracea
Antibodies
Enzymes
Electrons
Amino Acids

Keywords

  • Co-localization
  • Ferredoxin-NADP reductase
  • Glyceraldehyde-3-P dehydrogenase
  • Nearest neighbor analysis
  • Photosynthetic CO-fixation
  • Pisum sativum

ASJC Scopus subject areas

  • Structural Biology

Cite this

Co-localization of glyceraldehyde-3-phosphate dehydrogenase with ferredoxin-NADP reductase in pea leaf chloroplasts. / Negi, Surendra S.; Carol, Andrew A.; Pandya, Shivangi; Braun, Werner; Anderson, Louise E.

In: Journal of Structural Biology, Vol. 161, No. 1, 01.2008, p. 18-30.

Research output: Contribution to journalArticle

Negi, Surendra S. ; Carol, Andrew A. ; Pandya, Shivangi ; Braun, Werner ; Anderson, Louise E. / Co-localization of glyceraldehyde-3-phosphate dehydrogenase with ferredoxin-NADP reductase in pea leaf chloroplasts. In: Journal of Structural Biology. 2008 ; Vol. 161, No. 1. pp. 18-30.
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