Co-opting ATP-generating glycolytic enzyme PGK1 phosphoglycerate kinase facilitates the assembly of viral replicase complexes

K. Reddisiva Prasanth, Chingkai Chuang, Peter D. Nagy

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

The intricate interactions between viruses and hosts include exploitation of host cells for viral replication by using many cellular resources, metabolites and energy. Tomato bushy stunt virus (TBSV), similar to other (+)RNA viruses, induces major changes in infected cells that lead to the formation of large replication compartments consisting of aggregated peroxisomal and ER membranes. Yet, it is not known how TBSV obtains the energy to fuel these energy-consuming processes. In the current work, the authors discovered that TBSV co-opts the glycolytic ATP-generating Pgk1 phosphoglycerate kinase to facilitate the assembly of new viral replicase complexes. The recruitment of Pgk1 into the viral replication compartment is through direct interaction with the viral replication proteins. Altogether, we provide evidence that the ATP generated locally within the replication compartment by the co-opted Pgk1 is used to fuel the ATP-requirement of the co-opted heat shock protein 70 (Hsp70) chaperone, which is essential for the assembly of new viral replicase complexes and the activation of functional viral RNA-dependent RNA polymerase. The advantage of direct recruitment of Pgk1 into the virus replication compartment could be that the virus replicase assembly does not need to intensively compete with cellular processes for access to ATP. In addition, local production of ATP within the replication compartment could greatly facilitate the efficiency of Hsp70-driven replicase assembly by providing high ATP concentration within the replication compartment.

Original languageEnglish (US)
Article numbere1006689
JournalPLoS Pathogens
Volume13
Issue number10
DOIs
StatePublished - Oct 1 2017
Externally publishedYes

Fingerprint

Phosphoglycerate Kinase
Virus Assembly
Tombusvirus
Adenosine Triphosphate
Enzymes
HSP70 Heat-Shock Proteins
RNA Replicase
RNA Viruses
Viral RNA
Viral Proteins
Virus Replication
Viruses
Membranes

ASJC Scopus subject areas

  • Parasitology
  • Microbiology
  • Immunology
  • Molecular Biology
  • Genetics
  • Virology

Cite this

Co-opting ATP-generating glycolytic enzyme PGK1 phosphoglycerate kinase facilitates the assembly of viral replicase complexes. / Prasanth, K. Reddisiva; Chuang, Chingkai; Nagy, Peter D.

In: PLoS Pathogens, Vol. 13, No. 10, e1006689, 01.10.2017.

Research output: Contribution to journalArticle

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