Cochlin induced TREK-1 co-expression and annexin A2 secretion: Role in trabecular meshwork cell elongation and motility

Manik Goel, Adam E. Sienkiewicz, Renata Picciani, Richard K. Lee, Sanjoy K. Bhattacharya

Research output: Contribution to journalArticle

20 Citations (Scopus)

Abstract

Fluid flow through large interstitial spaces is sensed at the cellular level, and mechanistic responses to flow changes enables expansion or contraction of the cells modulating the surrounding area and brings about changes in fluid flow. In the anterior eye chamber, aqueous humor, a clear fluid, flows through trabecular meshwork (TM), a filter like region. Cochlin, a secreted protein in the extracellular matrix, was identified in the TM of glaucomatous patients but not controls by mass spectrometry. Cochlin undergoes shear induced multimerization and plays a role in mechanosensing of fluid shear. Cytoskeletal changes in response to mechanosensing in the ECM by cochlin will necessitate transduction of mechanosensing. TREK-1, a stretch activated outward rectifying potassium channel protein known to act as mechanotransducer was found to be expressed in TM. Cochlin expression results in co-expression of TREK-1 and filopodia formation. Prolonged cochlin expression results in expression and subsequent secretion of annexin A2, a protein known to play a role in cytoskeletal remodeling. Cochlin interacts with TREK-1 and annexin A2. Cochlin-TREK-1 interaction has functional consequences and results in changes in cell shape and motility. Annexin A2 expression and secretion follows cochlin-TREK-1 syn-expression and correlates with cell elongation. Thus cytoskeleton changes in response to fluid shear sensed by cochlin are further mediated by TREK-1 and annexin A2.

Original languageEnglish (US)
Article numbere23070
JournalPLoS One
Volume6
Issue number8
DOIs
StatePublished - Aug 23 2011
Externally publishedYes

Fingerprint

Annexin A2
Trabecular Meshwork
cell movement
Cell Movement
Elongation
cell growth
secretion
shears
Flow of fluids
pseudopodia
proteins
potassium channels
Military electronic countermeasures
cytoskeleton
Proteins
Pseudopodia
Fluids
extracellular matrix
Cell Shape
Aqueous Humor

ASJC Scopus subject areas

  • Agricultural and Biological Sciences(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Medicine(all)

Cite this

Cochlin induced TREK-1 co-expression and annexin A2 secretion : Role in trabecular meshwork cell elongation and motility. / Goel, Manik; Sienkiewicz, Adam E.; Picciani, Renata; Lee, Richard K.; Bhattacharya, Sanjoy K.

In: PLoS One, Vol. 6, No. 8, e23070, 23.08.2011.

Research output: Contribution to journalArticle

Goel, Manik ; Sienkiewicz, Adam E. ; Picciani, Renata ; Lee, Richard K. ; Bhattacharya, Sanjoy K. / Cochlin induced TREK-1 co-expression and annexin A2 secretion : Role in trabecular meshwork cell elongation and motility. In: PLoS One. 2011 ; Vol. 6, No. 8.
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