Coculture of meniscus cells and mesenchymal stem cells in simulated microgravity

William M. Weiss, Aillette Mulet-Sierra, Melanie Kunze, Nadr M. Jomha, Adetola B. Adesida

Research output: Contribution to journalArticlepeer-review

Abstract

Simulated microgravity has been shown to enhance cartilaginous matrix formation by chondrocytes and chondrogenesis of mesenchymal stem cells (MSCs). Similarly, coculture of primary chondrocytes with MSCs has been shown as a strategy to simultaneously retain the differentiated phenotype of chondrocytes and enhance cartilaginous matrix formation. In this study, we investigated the effect of simulated microgravity on cocultures of primary human meniscus cells and adipose-derived MSCs. We used biochemical, qPCR, and immunofluorescence assays to conduct our investigation. Simulated microgravity significantly enhanced cartilaginous matrix formation in cocultures of primary meniscus cells and adipose-derived MSCs. The enhancement was accompanied by increased hypertrophic differentiation markers, COL10A1 and MMP-13, and suppression of hypertrophic differentiation inhibitor, gremlin 1 (GREM1).

Original languageEnglish (US)
Article number28
Journalnpj Microgravity
Volume3
Issue number1
DOIs
StatePublished - Dec 1 2017
Externally publishedYes

ASJC Scopus subject areas

  • Medicine (miscellaneous)
  • Materials Science (miscellaneous)
  • Biochemistry, Genetics and Molecular Biology (miscellaneous)
  • Agricultural and Biological Sciences (miscellaneous)
  • Physics and Astronomy (miscellaneous)
  • Space and Planetary Science

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