Abstract
Thermal wave imaging of discrete subsurface chromophores in biological materials is reported using a phase sensitive coherent detection technique applied to recorded infrared (IR) images. We demonstrate that utilization of a periodically modulated laser source for thermal wave excitation and coherent detection applied to each pixel may be used to compute images of thermal wave amplitude and phase at the laser modulation frequency. In comparison to recorded IR images, the narrow-band detection technique significantly improves the quality of thermal wave amplitude images of subsurface chromophores in biological materials. Additionally, the technique provides phase information, which may be used to estimate chromophore depth in tissue. Application of the technique is demonstrated using tissue phantoms and in vivo biological models. We present a theoretical analysis and computer simulations that demonstrate the effect of tissue optical and thermal properties on thermal wave amplitude and phase. In comparison to the pulsed photothermal technique, coherent thermal wave imaging of subsurface chromophores in tissue for diagnostic applications allows reduction of peak incident laser fluence by as much as four orders of magnitude and is safer and more amenable to in vivo imaging.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 657-671 |
| Number of pages | 15 |
| Journal | Physics in Medicine and Biology |
| Volume | 47 |
| Issue number | 4 |
| DOIs | |
| State | Published - Feb 21 2002 |
| Externally published | Yes |
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ASJC Scopus subject areas
- Biomedical Engineering
- Physics and Astronomy (miscellaneous)
- Radiology Nuclear Medicine and imaging
- Radiological and Ultrasound Technology
Cite this
Coherent thermal wave imaging of subsurface chromophores in biological materials. / Telenkov, Sergey A.; Vargas, Gracie; Nelson, J. Stuart; Milner, Thomas E.
In: Physics in Medicine and Biology, Vol. 47, No. 4, 21.02.2002, p. 657-671.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Coherent thermal wave imaging of subsurface chromophores in biological materials
AU - Telenkov, Sergey A.
AU - Vargas, Gracie
AU - Nelson, J. Stuart
AU - Milner, Thomas E.
PY - 2002/2/21
Y1 - 2002/2/21
N2 - Thermal wave imaging of discrete subsurface chromophores in biological materials is reported using a phase sensitive coherent detection technique applied to recorded infrared (IR) images. We demonstrate that utilization of a periodically modulated laser source for thermal wave excitation and coherent detection applied to each pixel may be used to compute images of thermal wave amplitude and phase at the laser modulation frequency. In comparison to recorded IR images, the narrow-band detection technique significantly improves the quality of thermal wave amplitude images of subsurface chromophores in biological materials. Additionally, the technique provides phase information, which may be used to estimate chromophore depth in tissue. Application of the technique is demonstrated using tissue phantoms and in vivo biological models. We present a theoretical analysis and computer simulations that demonstrate the effect of tissue optical and thermal properties on thermal wave amplitude and phase. In comparison to the pulsed photothermal technique, coherent thermal wave imaging of subsurface chromophores in tissue for diagnostic applications allows reduction of peak incident laser fluence by as much as four orders of magnitude and is safer and more amenable to in vivo imaging.
AB - Thermal wave imaging of discrete subsurface chromophores in biological materials is reported using a phase sensitive coherent detection technique applied to recorded infrared (IR) images. We demonstrate that utilization of a periodically modulated laser source for thermal wave excitation and coherent detection applied to each pixel may be used to compute images of thermal wave amplitude and phase at the laser modulation frequency. In comparison to recorded IR images, the narrow-band detection technique significantly improves the quality of thermal wave amplitude images of subsurface chromophores in biological materials. Additionally, the technique provides phase information, which may be used to estimate chromophore depth in tissue. Application of the technique is demonstrated using tissue phantoms and in vivo biological models. We present a theoretical analysis and computer simulations that demonstrate the effect of tissue optical and thermal properties on thermal wave amplitude and phase. In comparison to the pulsed photothermal technique, coherent thermal wave imaging of subsurface chromophores in tissue for diagnostic applications allows reduction of peak incident laser fluence by as much as four orders of magnitude and is safer and more amenable to in vivo imaging.
UR - http://www.scopus.com/inward/record.url?scp=0037148659&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0037148659&partnerID=8YFLogxK
U2 - 10.1088/0031-9155/47/4/308
DO - 10.1088/0031-9155/47/4/308
M3 - Article
VL - 47
SP - 657
EP - 671
JO - Physics in Medicine and Biology
JF - Physics in Medicine and Biology
SN - 0031-9155
IS - 4
ER -