Collagenase-3 (MMP-13) in fetal membranes and amniotic fluid during pregnancy

Stephen J. Fortunato, Bonnie LaFleur, Ramkumar Menon

Research output: Contribution to journalArticle

28 Citations (Scopus)

Abstract

Objective: To examine the expression, site of production and a role of collagenase-3 in human fetal membranes and amniotic fluid (AF). Methods: Amniochorion collected at the time of elective repeat cesareans prior to labor from normal term gestation, were placed in an organ explant system for 72 hr. The AF was collected from the following groups of women: women at term; women at third trimester pregnancy: women at mid-trimester pregnancy. Women with premature rupture of the membranes (PROM) wifh or without pre-term labor and infection delivered by cesareans before term; women with pre-term labor and no rupture of membranes and delivered by cesareans before term; The mRNA expression of collagenase-3 was studied by reverse transcriptase-polymerase chain reaction (RT-PCR) and protein concentrations in the AF were assayed by enzyme-linked immunosorbent assay (ELISA). Site of collagenase-3 production was documented by immunohistochemistry. Statistical comparisons were made using ANOVA. All statistical hypotheses were adjusted for multiple comparisons using the Scheffe method of adjustment. Results: Amniochorion at term expressed collagenase-3 mRNA. Immunohistochemistry localized collagenase-3 in both amnion and chorion. Collagenase-3 was seen in AF from all gestational age groups with lowest levels at mid-trimester [167.8 pg/mL] and highest during term labor (323.92 pg/mL) with no statistically significant difference between term and third trimester (310.11 pg/mL). AF levels of collagenase-3 were significantly higher in women with pre-term labor (370.02 pg/mL; Padj = 0.046) or pre-term labor and infection (628.73 pg/mL; Padj = 0.002) compared with PROM with pre-term labor and infection (87.19 pg/mL). Conclusion: Collagenase-3 mRNA is constitutively expressed and produced in amniochorion. It is a normal physiologic constituent of AF. PROM is not associated with an increase in the AF collagenase-3.

Original languageEnglish (US)
Pages (from-to)120-125
Number of pages6
JournalAmerican Journal of Reproductive Immunology
Volume49
Issue number2
DOIs
StatePublished - Feb 1 2003
Externally publishedYes

Fingerprint

Matrix Metalloproteinase 13
Extraembryonic Membranes
Amniotic Fluid
Matrix Metalloproteinases
Pregnancy
Rupture
Membranes
Third Pregnancy Trimester
Messenger RNA
Infection
Immunohistochemistry
Pregnancy Trimesters
Chorion
Amnion
Reverse Transcriptase Polymerase Chain Reaction
Gestational Age
Analysis of Variance
Age Groups
Enzyme-Linked Immunosorbent Assay

Keywords

  • Amniochorion
  • Amniotic fluid
  • MMP-13
  • Pre-term labor
  • Premature rupture of the membranes

ASJC Scopus subject areas

  • Immunology
  • Obstetrics and Gynecology

Cite this

Collagenase-3 (MMP-13) in fetal membranes and amniotic fluid during pregnancy. / Fortunato, Stephen J.; LaFleur, Bonnie; Menon, Ramkumar.

In: American Journal of Reproductive Immunology, Vol. 49, No. 2, 01.02.2003, p. 120-125.

Research output: Contribution to journalArticle

@article{a0edf8c0aaa54503952f4b71b0696b71,
title = "Collagenase-3 (MMP-13) in fetal membranes and amniotic fluid during pregnancy",
abstract = "Objective: To examine the expression, site of production and a role of collagenase-3 in human fetal membranes and amniotic fluid (AF). Methods: Amniochorion collected at the time of elective repeat cesareans prior to labor from normal term gestation, were placed in an organ explant system for 72 hr. The AF was collected from the following groups of women: women at term; women at third trimester pregnancy: women at mid-trimester pregnancy. Women with premature rupture of the membranes (PROM) wifh or without pre-term labor and infection delivered by cesareans before term; women with pre-term labor and no rupture of membranes and delivered by cesareans before term; The mRNA expression of collagenase-3 was studied by reverse transcriptase-polymerase chain reaction (RT-PCR) and protein concentrations in the AF were assayed by enzyme-linked immunosorbent assay (ELISA). Site of collagenase-3 production was documented by immunohistochemistry. Statistical comparisons were made using ANOVA. All statistical hypotheses were adjusted for multiple comparisons using the Scheffe method of adjustment. Results: Amniochorion at term expressed collagenase-3 mRNA. Immunohistochemistry localized collagenase-3 in both amnion and chorion. Collagenase-3 was seen in AF from all gestational age groups with lowest levels at mid-trimester [167.8 pg/mL] and highest during term labor (323.92 pg/mL) with no statistically significant difference between term and third trimester (310.11 pg/mL). AF levels of collagenase-3 were significantly higher in women with pre-term labor (370.02 pg/mL; Padj = 0.046) or pre-term labor and infection (628.73 pg/mL; Padj = 0.002) compared with PROM with pre-term labor and infection (87.19 pg/mL). Conclusion: Collagenase-3 mRNA is constitutively expressed and produced in amniochorion. It is a normal physiologic constituent of AF. PROM is not associated with an increase in the AF collagenase-3.",
keywords = "Amniochorion, Amniotic fluid, MMP-13, Pre-term labor, Premature rupture of the membranes",
author = "Fortunato, {Stephen J.} and Bonnie LaFleur and Ramkumar Menon",
year = "2003",
month = "2",
day = "1",
doi = "10.1034/j.1600-0897.2003.00012.x",
language = "English (US)",
volume = "49",
pages = "120--125",
journal = "American Journal of Reproductive Immunology and Microbiology",
issn = "1046-7408",
publisher = "Wiley-Blackwell",
number = "2",

}

TY - JOUR

T1 - Collagenase-3 (MMP-13) in fetal membranes and amniotic fluid during pregnancy

AU - Fortunato, Stephen J.

AU - LaFleur, Bonnie

AU - Menon, Ramkumar

PY - 2003/2/1

Y1 - 2003/2/1

N2 - Objective: To examine the expression, site of production and a role of collagenase-3 in human fetal membranes and amniotic fluid (AF). Methods: Amniochorion collected at the time of elective repeat cesareans prior to labor from normal term gestation, were placed in an organ explant system for 72 hr. The AF was collected from the following groups of women: women at term; women at third trimester pregnancy: women at mid-trimester pregnancy. Women with premature rupture of the membranes (PROM) wifh or without pre-term labor and infection delivered by cesareans before term; women with pre-term labor and no rupture of membranes and delivered by cesareans before term; The mRNA expression of collagenase-3 was studied by reverse transcriptase-polymerase chain reaction (RT-PCR) and protein concentrations in the AF were assayed by enzyme-linked immunosorbent assay (ELISA). Site of collagenase-3 production was documented by immunohistochemistry. Statistical comparisons were made using ANOVA. All statistical hypotheses were adjusted for multiple comparisons using the Scheffe method of adjustment. Results: Amniochorion at term expressed collagenase-3 mRNA. Immunohistochemistry localized collagenase-3 in both amnion and chorion. Collagenase-3 was seen in AF from all gestational age groups with lowest levels at mid-trimester [167.8 pg/mL] and highest during term labor (323.92 pg/mL) with no statistically significant difference between term and third trimester (310.11 pg/mL). AF levels of collagenase-3 were significantly higher in women with pre-term labor (370.02 pg/mL; Padj = 0.046) or pre-term labor and infection (628.73 pg/mL; Padj = 0.002) compared with PROM with pre-term labor and infection (87.19 pg/mL). Conclusion: Collagenase-3 mRNA is constitutively expressed and produced in amniochorion. It is a normal physiologic constituent of AF. PROM is not associated with an increase in the AF collagenase-3.

AB - Objective: To examine the expression, site of production and a role of collagenase-3 in human fetal membranes and amniotic fluid (AF). Methods: Amniochorion collected at the time of elective repeat cesareans prior to labor from normal term gestation, were placed in an organ explant system for 72 hr. The AF was collected from the following groups of women: women at term; women at third trimester pregnancy: women at mid-trimester pregnancy. Women with premature rupture of the membranes (PROM) wifh or without pre-term labor and infection delivered by cesareans before term; women with pre-term labor and no rupture of membranes and delivered by cesareans before term; The mRNA expression of collagenase-3 was studied by reverse transcriptase-polymerase chain reaction (RT-PCR) and protein concentrations in the AF were assayed by enzyme-linked immunosorbent assay (ELISA). Site of collagenase-3 production was documented by immunohistochemistry. Statistical comparisons were made using ANOVA. All statistical hypotheses were adjusted for multiple comparisons using the Scheffe method of adjustment. Results: Amniochorion at term expressed collagenase-3 mRNA. Immunohistochemistry localized collagenase-3 in both amnion and chorion. Collagenase-3 was seen in AF from all gestational age groups with lowest levels at mid-trimester [167.8 pg/mL] and highest during term labor (323.92 pg/mL) with no statistically significant difference between term and third trimester (310.11 pg/mL). AF levels of collagenase-3 were significantly higher in women with pre-term labor (370.02 pg/mL; Padj = 0.046) or pre-term labor and infection (628.73 pg/mL; Padj = 0.002) compared with PROM with pre-term labor and infection (87.19 pg/mL). Conclusion: Collagenase-3 mRNA is constitutively expressed and produced in amniochorion. It is a normal physiologic constituent of AF. PROM is not associated with an increase in the AF collagenase-3.

KW - Amniochorion

KW - Amniotic fluid

KW - MMP-13

KW - Pre-term labor

KW - Premature rupture of the membranes

UR - http://www.scopus.com/inward/record.url?scp=0037507321&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0037507321&partnerID=8YFLogxK

U2 - 10.1034/j.1600-0897.2003.00012.x

DO - 10.1034/j.1600-0897.2003.00012.x

M3 - Article

VL - 49

SP - 120

EP - 125

JO - American Journal of Reproductive Immunology and Microbiology

JF - American Journal of Reproductive Immunology and Microbiology

SN - 1046-7408

IS - 2

ER -