Collagenase activity in rabbit carcinoma

Cell source and cell interactions

M. K. Dabbous, M. El-Torky, L. Haney, S. B. Brinkley, N. Sobhy

Research output: Contribution to journalArticle

37 Citations (Scopus)

Abstract

Fibroblast-like cells (F-cells) and epithelial-like (E-cells) derived from cultures of rabbit VX-2 carcinoma released collagenase in both active and latent forms in serum-free medium at a level higher than that of normal rabbit fibroblast cultures. The enhanced capacity of the F-cells to release the enzyme, however, continued only for a few passages and then decreased significantly to a low level similar to that of the normal fibroblasts. The enzyme-release by the E-cells continued for a few more passages at a relatively moderate level, higher than that of normal fibroblasts. The release of collagenase in cultures of F-cells was enhanced by the presence of E-cells in mixed cultures as well as by medium conditioned by the E-cells type. Addition of cytochalasin B at 2 μg/ml did not significantly effect the enzyme activity released in the cultures. Serum from tumor-bearing rabbits appeared to stimulate the release of enzyme activity in cultures of either cell type.

Original languageEnglish (US)
Pages (from-to)357-364
Number of pages8
JournalInternational Journal of Cancer
Volume31
Issue number3
StatePublished - 1983
Externally publishedYes

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Collagenases
Cell Communication
Fibroblasts
Rabbits
Carcinoma
Enzymes
Cell Culture Techniques
Cytochalasin B
Serum-Free Culture Media
Conditioned Culture Medium
Epithelial Cells
Serum
Neoplasms

ASJC Scopus subject areas

  • Cancer Research
  • Oncology

Cite this

Dabbous, M. K., El-Torky, M., Haney, L., Brinkley, S. B., & Sobhy, N. (1983). Collagenase activity in rabbit carcinoma: Cell source and cell interactions. International Journal of Cancer, 31(3), 357-364.

Collagenase activity in rabbit carcinoma : Cell source and cell interactions. / Dabbous, M. K.; El-Torky, M.; Haney, L.; Brinkley, S. B.; Sobhy, N.

In: International Journal of Cancer, Vol. 31, No. 3, 1983, p. 357-364.

Research output: Contribution to journalArticle

Dabbous, MK, El-Torky, M, Haney, L, Brinkley, SB & Sobhy, N 1983, 'Collagenase activity in rabbit carcinoma: Cell source and cell interactions', International Journal of Cancer, vol. 31, no. 3, pp. 357-364.
Dabbous MK, El-Torky M, Haney L, Brinkley SB, Sobhy N. Collagenase activity in rabbit carcinoma: Cell source and cell interactions. International Journal of Cancer. 1983;31(3):357-364.
Dabbous, M. K. ; El-Torky, M. ; Haney, L. ; Brinkley, S. B. ; Sobhy, N. / Collagenase activity in rabbit carcinoma : Cell source and cell interactions. In: International Journal of Cancer. 1983 ; Vol. 31, No. 3. pp. 357-364.
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