Colloidal gold labeling of sections and cell surfaces

Hal K. Hawkins, Linda S. Rehm, Jessica Y. Zhu

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

Colloidal gold labeling techniques can be simplified enough to become practical for diagnostic use in a hospital electron microscopy laboratory. Methods found to be practical and reproducible are presented, including production of colloidal gold reagents, polar embedding for gold labeling of thin sections, and use of silver enhancement on semithin sections. Methods for cell surface labeling are emphasized; they are technically straightforward and can be applied to investigation of multiple diagnostic problems.

Original languageEnglish (US)
Pages (from-to)61-70
Number of pages10
JournalUltrastructural Pathology
Volume16
Issue number1-2
DOIs
StatePublished - 1992
Externally publishedYes

Fingerprint

Gold Colloid
Labeling
marking
Gold
gold
cells
Silver
Electron Microscopy
embedding
Electron microscopy
reagents
electron microscopy
silver
augmentation

Keywords

  • Adhesion molecules
  • Colloidal gold
  • Immunohistochemistry
  • Mac-1
  • Methods
  • Receptors

ASJC Scopus subject areas

  • Pathology and Forensic Medicine
  • Structural Biology
  • Instrumentation

Cite this

Colloidal gold labeling of sections and cell surfaces. / Hawkins, Hal K.; Rehm, Linda S.; Zhu, Jessica Y.

In: Ultrastructural Pathology, Vol. 16, No. 1-2, 1992, p. 61-70.

Research output: Contribution to journalArticle

Hawkins, Hal K. ; Rehm, Linda S. ; Zhu, Jessica Y. / Colloidal gold labeling of sections and cell surfaces. In: Ultrastructural Pathology. 1992 ; Vol. 16, No. 1-2. pp. 61-70.
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