Colloidal gold labeling of sections and cell surfaces

Hal K. Hawkins, Linda S. Rehm, Jessica Y. Zhu

Research output: Contribution to journalArticle

4 Scopus citations

Abstract

Colloidal gold labeling techniques can be simplified enough to become practical for diagnostic use in a hospital electron microscopy laboratory. Methods found to be practical and reproducible are presented, including production of colloidal gold reagents, polar embedding for gold labeling of thin sections, and use of silver enhancement on semithin sections. Methods for cell surface labeling are emphasized; they are technically straightforward and can be applied to investigation of multiple diagnostic problems.

Original languageEnglish (US)
Pages (from-to)61-70
Number of pages10
JournalUltrastructural Pathology
Volume16
Issue number1-2
DOIs
StatePublished - 1992
Externally publishedYes

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Keywords

  • Adhesion molecules
  • Colloidal gold
  • Immunohistochemistry
  • Mac-1
  • Methods
  • Receptors

ASJC Scopus subject areas

  • Pathology and Forensic Medicine
  • Structural Biology
  • Instrumentation

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