Colocalization of calcitonin gene-related peptide and somatostatin in pancreatic islet cells and inhibition of insulin secretion by calcitonin gene-related peptide in the rat

Masaki Fujimura, George H. Greeley, Michael B. Hancock, Anders Alwmark, Alejandro Santos, Cary W. Cooper, Karen J. Reumont, Jin Ishizuka, James C. Thompson

    Research output: Contribution to journalArticle

    36 Scopus citations

    Abstract

    Calcitonin gene-related peptide (CGRP)-and somatostatin (SRlF)-containing cells were identified by immunocytochemical techniques in pancreatic islet cells of the rat. CGRP-containing cells were found primarily in the peripheral portion of the pancreatic islets. In addition, CGRP-containing cells also contained somatostatin, which identifies the islet CGRP-containing cells as D cells. In the present study, we also tested the effect of CGRP on gastrin-releasing peptide (GRP; 10-9M)-or cholecystokinin (CCK-8, 10-9M)-stimulated release of insulin from isolated rat islets in vitro. At concentrations of 10-8-10-11M, CGRP inhibited GRP-and CCK-8-stimulated release of insulin significantly when compared with GRP or CCK-8 alone. At the lowest concentration of CGRP (10-11M), the inhibitory effect of CGRP on CCK-8-stimulated release of insulin was statistically significant (p < 0.05) and exceptionally potent (65-90% inhibition). We have also found that CGRP does not stimulate the release of SRIF from isolated islet cells. These findings suggest that CGRP may play a regulatory role in the release of insulin.

    Original languageEnglish (US)
    Pages (from-to)49-52
    Number of pages4
    JournalPancreas
    Volume3
    Issue number1
    DOIs
    StatePublished - Feb 1988

    Keywords

    • Calcitonin gene-related peptide

    ASJC Scopus subject areas

    • Internal Medicine
    • Endocrinology, Diabetes and Metabolism
    • Hepatology
    • Endocrinology

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    Fujimura, M., Greeley, G. H., Hancock, M. B., Alwmark, A., Santos, A., Cooper, C. W., Reumont, K. J., Ishizuka, J., & Thompson, J. C. (1988). Colocalization of calcitonin gene-related peptide and somatostatin in pancreatic islet cells and inhibition of insulin secretion by calcitonin gene-related peptide in the rat. Pancreas, 3(1), 49-52. https://doi.org/10.1097/00006676-198802000-00009