Colocalization of calcitonin gene-related peptide and somatostatin in pancreatic islet cells and inhibition of insulin secretion by calcitonin gene-related peptide in the rat

M. Fujimura, G. H. Greeley, M. B. Hancock, A. Alwmark, A. Santos, C. W. Cooper, K. J. Reumont, J. Ishizuka, J. C. Thompson

Research output: Contribution to journalArticle

37 Scopus citations

Abstract

Calcitonin gene-related peptide (CGRP)- and somatostatin (SRIF)-containing cells were identified by immunocytochemical techniques in pancreatic islet cells of the rat. CGRP-containing cells were found primarily in the peripheral portion of the pancreatic islets. In addition, CGRP-containing cells also contained somatostatin, which identifies the islet CGRP-containing cells as D cells. In the present study, we also tested the effect of CGRP on gastrin-releasing peptide (GRP; 10-9 M)- or cholecystokinin (CCK-8, 10-9 M)-stimulated release of insulin from isolated rat islets in vitro. At concentrations of 10-8-10-11 M, CGRP inhibited GRP- and CCK-8-stimulated release of insulin significantly when compared with GRP or CCK-8 alone. At the lowest concentration of CGRP (10-11 M), the inhibitory effect of CGRP on CCK-8-stimulated release of insulin was statistically significant (p < 0.05) and exceptionally potent (65-90% inhibition). We have also found that CGRP does not stimulate the release of SRIF from isolated islet cells. These findings suggest that CGRP may play a regulatory role in the release of insulin.

Original languageEnglish (US)
Pages (from-to)49-52
Number of pages4
JournalPancreas
Volume3
Issue number1
StatePublished - 1988

    Fingerprint

ASJC Scopus subject areas

  • Endocrinology
  • Gastroenterology

Cite this

Fujimura, M., Greeley, G. H., Hancock, M. B., Alwmark, A., Santos, A., Cooper, C. W., Reumont, K. J., Ishizuka, J., & Thompson, J. C. (1988). Colocalization of calcitonin gene-related peptide and somatostatin in pancreatic islet cells and inhibition of insulin secretion by calcitonin gene-related peptide in the rat. Pancreas, 3(1), 49-52.