Colonized sabethes cyaneus, a sylvatic new world mosquito species, shows a low vector competence for zika virus relative to aedes aegypti

Ajit K. Karna, Sasha R. Azar, Jessica Plante, Rumei Yun, Nikos Vasilakis, Scott Weaver, Immo A. Hansen, Kathryn A. Hanley

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

The introduction of Zika virus (ZIKV) to the Americas raised concern that the virus would spill back from human transmission, perpetuated by Aedes aegypti, into a sylvatic cycle maintained in wildlife and forest-living mosquitoes. In the Americas, Sabethes species are vectors of sylvatic yellow fever virus (YFV) and are therefore candidate vectors of a sylvatic ZIKV cycle. To test the potential of Sabethes cyaneus to transmit ZIKV, Sa. cyaneus and Ae. aegypti were fed on A129 mice one or two days post-infection (dpi) with a ZIKV isolate from Mexico. Sa. cyaneus were sampled at 3, 4, 5, 7, 14, and 21 days post-feeding (dpf) and Ae. aegypti were sampled at 14 and 21 dpf. ZIKV was quantified in mosquito bodies, legs, and saliva to measure infection, dissemination, and potential transmission, respectively. Of 69 Sa. cyaneus that fed, ZIKV was detected in only one, in all body compartments, at 21 dpf. In contrast, at 14 dpf 100% of 20 Ae. aegypti that fed on mice at 2 dpi were infected and 70% had virus in saliva. These data demonstrate that Sa. cyaneus is a competent vector for ZIKV, albeit much less competent than Ae. aegypti.

Original languageEnglish (US)
Article number434
JournalViruses
Volume10
Issue number8
DOIs
StatePublished - Aug 16 2018

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Aedes
Culicidae
Mental Competency
Saliva
Infection
Yellow fever virus
Viruses
Mexico
Zika Virus
Leg

Keywords

  • Aedes aegypti
  • Americas
  • Sabethes cyaneus
  • Spillback
  • Sylvatic cycle
  • Vector competence
  • Zika virus

ASJC Scopus subject areas

  • Infectious Diseases
  • Virology

Cite this

Colonized sabethes cyaneus, a sylvatic new world mosquito species, shows a low vector competence for zika virus relative to aedes aegypti. / Karna, Ajit K.; Azar, Sasha R.; Plante, Jessica; Yun, Rumei; Vasilakis, Nikos; Weaver, Scott; Hansen, Immo A.; Hanley, Kathryn A.

In: Viruses, Vol. 10, No. 8, 434, 16.08.2018.

Research output: Contribution to journalArticle

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abstract = "The introduction of Zika virus (ZIKV) to the Americas raised concern that the virus would spill back from human transmission, perpetuated by Aedes aegypti, into a sylvatic cycle maintained in wildlife and forest-living mosquitoes. In the Americas, Sabethes species are vectors of sylvatic yellow fever virus (YFV) and are therefore candidate vectors of a sylvatic ZIKV cycle. To test the potential of Sabethes cyaneus to transmit ZIKV, Sa. cyaneus and Ae. aegypti were fed on A129 mice one or two days post-infection (dpi) with a ZIKV isolate from Mexico. Sa. cyaneus were sampled at 3, 4, 5, 7, 14, and 21 days post-feeding (dpf) and Ae. aegypti were sampled at 14 and 21 dpf. ZIKV was quantified in mosquito bodies, legs, and saliva to measure infection, dissemination, and potential transmission, respectively. Of 69 Sa. cyaneus that fed, ZIKV was detected in only one, in all body compartments, at 21 dpf. In contrast, at 14 dpf 100{\%} of 20 Ae. aegypti that fed on mice at 2 dpi were infected and 70{\%} had virus in saliva. These data demonstrate that Sa. cyaneus is a competent vector for ZIKV, albeit much less competent than Ae. aegypti.",
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