Colostrinin-driven neurite outgrowth requires p53 activation in PC12 cells

Attila Bacsi, G. John Stanton, Thomas K. Hughes, Marian Kruze, Istvan Boldogh

Research output: Contribution to journalArticle

30 Scopus citations

Abstract

1. Colostrinin (CLN) induces maturation and differentiation of murine thymocytes, promotes proliferation of peripheral blood leukocytes, induces immunomodulator cytokines, and ameliorates oxidative stress-mediated activation of c-Jun NH2-terminal kinases. 2. Here we report that upon treatment with CLN, medullary pheochromocytoma (PC12) cells ceased to proliferate and extend neurites. 3. The arrest of CLN-treated PC12 cells in the G1 phase of the cell cycle was due to an increase in the phosphorylation of p53 at serine 15 (p53 ser15 ) and expression of p21 WAF1 . PC12 cells treated with inhibitory oligonucleotides to p53 lacked p53 ser15 and p21 WAF1 expression, and did not show morphological changes after CLN exposure. Transfection with inhibitory oligonucleotides to p21 WAF1 had no effect on p53 activation; however, cells failed to arrest or extend neurites. An oligonucleotide inhibiting luciferase expression had no effect on CLN-mediated p53 activation, p21 WAF1 expression, growth arrest, or neurite outgrowth. 4. We conclude that CLN induces delicate cassettes of signaling pathways common to cell proliferation and differentiation, and mediates activities that are similar to those of hormones and neurotrophins, leading to neurite outgrowth.

Original languageEnglish (US)
Pages (from-to)1123-1139
Number of pages17
JournalCellular and Molecular Neurobiology
Volume25
Issue number7
DOIs
StatePublished - Nov 2005

Keywords

  • Colostrinin
  • Neurite outgrowth
  • Pheochromocytoma cell
  • p53 activation

ASJC Scopus subject areas

  • Cellular and Molecular Neuroscience
  • Cell Biology

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