Colostrinin-driven neurite outgrowth requires p53 activation in PC12 cells

Attila Bacsi, G. John Stanton, Thomas K. Hughes, Marian Kruzel, Istvan Boldogh

Research output: Contribution to journalArticle

30 Citations (Scopus)

Abstract

1. Colostrinin (CLN) induces maturation and differentiation of murine thymocytes, promotes proliferation of peripheral blood leukocytes, induces immunomodulator cytokines, and ameliorates oxidative stress-mediated activation of c-Jun NH2-terminal kinases. 2. Here we report that upon treatment with CLN, medullary pheochromocytoma (PC12) cells ceased to proliferate and extend neurites. 3. The arrest of CLN-treated PC12 cells in the G1 phase of the cell cycle was due to an increase in the phosphorylation of p53 at serine 15 (p53 ser15) and expression of p21 WAF1. PC12 cells treated with inhibitory oligonucleotides to p53 lacked p53 ser15 and p21 WAF1 expression, and did not show morphological changes after CLN exposure. Transfection with inhibitory oligonucleotides to p21 WAF1 had no effect on p53 activation; however, cells failed to arrest or extend neurites. An oligonucleotide inhibiting luciferase expression had no effect on CLN-mediated p53 activation, p21 WAF1 expression, growth arrest, or neurite outgrowth. 4. We conclude that CLN induces delicate cassettes of signaling pathways common to cell proliferation and differentiation, and mediates activities that are similar to those of hormones and neurotrophins, leading to neurite outgrowth.

Original languageEnglish (US)
Pages (from-to)1123-1139
Number of pages17
JournalCellular and Molecular Neurobiology
Volume25
Issue number7
DOIs
StatePublished - Nov 2005

Fingerprint

PC12 Cells
Chemical activation
Oligonucleotides
Neurites
Serine
Phosphorylation
Oxidative stress
JNK Mitogen-Activated Protein Kinases
Nerve Growth Factors
Cell proliferation
Immunologic Factors
G1 Phase
Pheochromocytoma
Thymocytes
Luciferases
Transfection
colostrinine
Neuronal Outgrowth
Cell Differentiation
Cell Cycle

Keywords

  • Colostrinin
  • Neurite outgrowth
  • p53 activation
  • Pheochromocytoma cell

ASJC Scopus subject areas

  • Neuroscience(all)
  • Clinical Biochemistry
  • Cell Biology
  • Genetics

Cite this

Colostrinin-driven neurite outgrowth requires p53 activation in PC12 cells. / Bacsi, Attila; Stanton, G. John; Hughes, Thomas K.; Kruzel, Marian; Boldogh, Istvan.

In: Cellular and Molecular Neurobiology, Vol. 25, No. 7, 11.2005, p. 1123-1139.

Research output: Contribution to journalArticle

Bacsi, Attila ; Stanton, G. John ; Hughes, Thomas K. ; Kruzel, Marian ; Boldogh, Istvan. / Colostrinin-driven neurite outgrowth requires p53 activation in PC12 cells. In: Cellular and Molecular Neurobiology. 2005 ; Vol. 25, No. 7. pp. 1123-1139.
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