Combination of widefield fluorescence imaging and nonlinear optical microscopy of oral epithelial neoplasia

Rahul Pal, Kert Edward, Tyra Brown, Liang Ma, Jinping Yang, Susan McCammon, Massoud Motamedi, Gracie Vargas

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

Multiphoton Autofluorescence Microscopy (MPAM) and Second Harmonic Generation Microscopy (SHGM) have shown the potential for noninvasive assessment of oral precancers and cancers. We have explored a combination of these nonlinear optical microscopic imaging techniques with widefield fluorescence imaging to assess morphometry similar to that of pathologic evaluation as well as information from endogenous fluorophores, which are altered with neoplastic transformation. Widefield fluorescence revealed areas of interest corresponding to sites with precancers or early tumors, generally resulting in a decrease in green emission or increase in red emission. Subsequent microscopy revealed significant differences in morphology between normal, dysplastic/neoplastic mucosa for all layers. Combination of a widefield and a microscopic technique provides a novel approach for tissue morphometric analysis along with large area assessment of tissue autofluorescence properties.

Original languageEnglish (US)
Title of host publicationProgress in Biomedical Optics and Imaging - Proceedings of SPIE
Volume8572
DOIs
StatePublished - 2013
EventAdvanced Biomedical and Clinical Diagnostic Systems XI - San Francisco, CA, United States
Duration: Feb 3 2013Feb 5 2013

Other

OtherAdvanced Biomedical and Clinical Diagnostic Systems XI
CountryUnited States
CitySan Francisco, CA
Period2/3/132/5/13

Fingerprint

Optical Imaging
Optical microscopy
Microscopy
Microscopic examination
Fluorescence
microscopy
Imaging techniques
fluorescence
Tissue
Neoplasms
Fluorophores
Mouth Neoplasms
Harmonic generation
imaging techniques
Tumors
harmonic generations
Mucous Membrane
tumors
cancer
evaluation

Keywords

  • Multiphoton microscopy
  • oral precancer
  • second harmonic generation microscopy
  • wide-field fluorescence imaging

ASJC Scopus subject areas

  • Atomic and Molecular Physics, and Optics
  • Electronic, Optical and Magnetic Materials
  • Biomaterials
  • Radiology Nuclear Medicine and imaging

Cite this

Pal, R., Edward, K., Brown, T., Ma, L., Yang, J., McCammon, S., ... Vargas, G. (2013). Combination of widefield fluorescence imaging and nonlinear optical microscopy of oral epithelial neoplasia. In Progress in Biomedical Optics and Imaging - Proceedings of SPIE (Vol. 8572). [85720M] https://doi.org/10.1117/12.2005144

Combination of widefield fluorescence imaging and nonlinear optical microscopy of oral epithelial neoplasia. / Pal, Rahul; Edward, Kert; Brown, Tyra; Ma, Liang; Yang, Jinping; McCammon, Susan; Motamedi, Massoud; Vargas, Gracie.

Progress in Biomedical Optics and Imaging - Proceedings of SPIE. Vol. 8572 2013. 85720M.

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Pal, R, Edward, K, Brown, T, Ma, L, Yang, J, McCammon, S, Motamedi, M & Vargas, G 2013, Combination of widefield fluorescence imaging and nonlinear optical microscopy of oral epithelial neoplasia. in Progress in Biomedical Optics and Imaging - Proceedings of SPIE. vol. 8572, 85720M, Advanced Biomedical and Clinical Diagnostic Systems XI, San Francisco, CA, United States, 2/3/13. https://doi.org/10.1117/12.2005144
Pal R, Edward K, Brown T, Ma L, Yang J, McCammon S et al. Combination of widefield fluorescence imaging and nonlinear optical microscopy of oral epithelial neoplasia. In Progress in Biomedical Optics and Imaging - Proceedings of SPIE. Vol. 8572. 2013. 85720M https://doi.org/10.1117/12.2005144
Pal, Rahul ; Edward, Kert ; Brown, Tyra ; Ma, Liang ; Yang, Jinping ; McCammon, Susan ; Motamedi, Massoud ; Vargas, Gracie. / Combination of widefield fluorescence imaging and nonlinear optical microscopy of oral epithelial neoplasia. Progress in Biomedical Optics and Imaging - Proceedings of SPIE. Vol. 8572 2013.
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