Combinations and loads of bacteria affect the cytokine production by fetal membranes

An in vitro study

Nathalia Mayumi Noda-Nicolau, Jossimara Polettini, Morgan R. Peltier, Márcia Guimarães da Silva, Ramkumar Menon

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

Problem: The polybacterial invasion and inflammation of the amniotic cavity is a common scenario in PTB, and then, we analyzed the cytokine production by human fetal membranes to better understand the host response to polybacterial infections. Method of study: Fetal membranes were treated by heat-inactivated genital mycoplasmas and Gardnerella vaginalis at 103 or 106 colony/color-forming units/mL alone or in combination. Cytokines/receptors were measured in the medium by immunoassays. Results: Stimulation of genital mycoplasmas did not increase the proinflammatory cytokines, except Ureaplasma urealyticum that increased IL-8 levels. However, U. urealyticum and Mycoplasma hominis significantly increased IL-10 and IL-13 levels. G. vaginalis alone or in combination with genital mycoplasmas showed an increased proinflammatory and anti-inflammatory cytokines. Conclusions: G. vaginalis sustain a proinflammatory response in the fetal membranes in vitro, while genital mycoplasmas induce a strong control of the inflammatory response. The ability of genital mycoplasmas to control the proinflammatory response may favor their survival in the upper genital tract.

Original languageEnglish (US)
Pages (from-to)504-511
Number of pages8
JournalAmerican Journal of Reproductive Immunology
Volume76
Issue number6
DOIs
StatePublished - Dec 1 2016

Fingerprint

Extraembryonic Membranes
Mycoplasma
Gardnerella vaginalis
Cytokines
Bacteria
Ureaplasma urealyticum
Mycoplasma hominis
Cytokine Receptors
Interleukin-13
Interleukin-8
Immunoassay
Interleukin-10
Anti-Inflammatory Agents
Stem Cells
Color
Hot Temperature
In Vitro Techniques
Inflammation
Survival
Infection

Keywords

  • Gardnerella vaginalis
  • genital mycoplasmas
  • polybacterial infection

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology
  • Reproductive Medicine
  • Obstetrics and Gynecology

Cite this

Combinations and loads of bacteria affect the cytokine production by fetal membranes : An in vitro study. / Noda-Nicolau, Nathalia Mayumi; Polettini, Jossimara; Peltier, Morgan R.; da Silva, Márcia Guimarães; Menon, Ramkumar.

In: American Journal of Reproductive Immunology, Vol. 76, No. 6, 01.12.2016, p. 504-511.

Research output: Contribution to journalArticle

Noda-Nicolau, Nathalia Mayumi ; Polettini, Jossimara ; Peltier, Morgan R. ; da Silva, Márcia Guimarães ; Menon, Ramkumar. / Combinations and loads of bacteria affect the cytokine production by fetal membranes : An in vitro study. In: American Journal of Reproductive Immunology. 2016 ; Vol. 76, No. 6. pp. 504-511.
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