Combinatorial selection and edited combinatorial selection of phosphorothioate aptamers targeting human nuclear factor-κB RelA/p50 and RelA/RelA

Suzanne E. Bassett, Susan M. Fennewald, David J. King, Xin Li, Norbert K. Herzog, Robert Shope, Judith Aronson, Bruce A. Luxon, David G. Gorenstein

Research output: Contribution to journalArticle

25 Citations (Scopus)

Abstract

Nuclear factor-κB (NF-κB) transcription factors are important in regulating the immune response and play critical roles in the pathogenesis of chronic inflammatory diseases and a variety of human cancers. Agents that target specific NF-κB dimers may serve as therapeutic agents for the prevention of pathogenic immune responses. We have selected monothiophosphate- modified aptamers, or "thioaptamers", to the NF-κB p50/RelA heterodimer using combinatorial selection techniques. We also utilized a "double sieve" or editing approach for the generation of thioaptamers with enhanced selectivity to the RelA/RelA homodimer. The thioaptamers from these selections and our previous selections on the p50/p50 and RelA/RelA homodimers all had unique sequences and bound tightly to the recombinant NF-κB dimers against which they were selected. The selected thioaptamers also appear to maintain their selectivity and specificity among other cellular proteins, because they have the ability to bind NF-κB proteins within nuclear extracts from lipopolysaccharide (LPS)-induced macrophages and B cells.

Original languageEnglish (US)
Pages (from-to)9105-9115
Number of pages11
JournalBiochemistry
Volume43
Issue number28
DOIs
StatePublished - Jul 20 2004

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Nuclear Proteins
Dimers
Lipopolysaccharides
B-Lymphocytes
Chronic Disease
Transcription Factors
Macrophages
Sieves
Neoplasms
Proteins
Cells
Therapeutics
3'-(1-butylphosphoryl)adenosine

ASJC Scopus subject areas

  • Biochemistry

Cite this

Bassett, S. E., Fennewald, S. M., King, D. J., Li, X., Herzog, N. K., Shope, R., ... Gorenstein, D. G. (2004). Combinatorial selection and edited combinatorial selection of phosphorothioate aptamers targeting human nuclear factor-κB RelA/p50 and RelA/RelA. Biochemistry, 43(28), 9105-9115. https://doi.org/10.1021/bi036220h

Combinatorial selection and edited combinatorial selection of phosphorothioate aptamers targeting human nuclear factor-κB RelA/p50 and RelA/RelA. / Bassett, Suzanne E.; Fennewald, Susan M.; King, David J.; Li, Xin; Herzog, Norbert K.; Shope, Robert; Aronson, Judith; Luxon, Bruce A.; Gorenstein, David G.

In: Biochemistry, Vol. 43, No. 28, 20.07.2004, p. 9105-9115.

Research output: Contribution to journalArticle

Bassett, SE, Fennewald, SM, King, DJ, Li, X, Herzog, NK, Shope, R, Aronson, J, Luxon, BA & Gorenstein, DG 2004, 'Combinatorial selection and edited combinatorial selection of phosphorothioate aptamers targeting human nuclear factor-κB RelA/p50 and RelA/RelA', Biochemistry, vol. 43, no. 28, pp. 9105-9115. https://doi.org/10.1021/bi036220h
Bassett, Suzanne E. ; Fennewald, Susan M. ; King, David J. ; Li, Xin ; Herzog, Norbert K. ; Shope, Robert ; Aronson, Judith ; Luxon, Bruce A. ; Gorenstein, David G. / Combinatorial selection and edited combinatorial selection of phosphorothioate aptamers targeting human nuclear factor-κB RelA/p50 and RelA/RelA. In: Biochemistry. 2004 ; Vol. 43, No. 28. pp. 9105-9115.
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