Combinatorial selection of a RNA thioaptamer that binds to Venezuelan equine encephalitis virus capsid protein

Jonghoon Kang, Myung Soog Lee, Stanley Watowich, David G. Gorenstein

Research output: Contribution to journalArticle

22 Citations (Scopus)

Abstract

A phosphorothioate RNA aptamer (thioaptamer) targeting the capsid protein of Venezuelan equine encephalitis virus (VEEV) was isolated by in vitro combinatorial selection. The selected thioaptamer had a strong binding affinity (∼7 nM) and high specificity for the target protein. For the binding to the protein, the overall tertiary structure of the thioaptamer is required. We introduce two theoretical methods to examine the effect of phosphorothioate modification on the enhancement of binding affinity and one experimental method to examine the nature of the multiple bands of thioaptamer in a native gel.

Original languageEnglish (US)
Pages (from-to)2497-2502
Number of pages6
JournalFEBS Letters
Volume581
Issue number13
DOIs
StatePublished - May 29 2007

Fingerprint

Venezuelan Equine Encephalitis Viruses
Capsid Proteins
Viruses
Nucleotide Aptamers
RNA
Carrier Proteins
Proteins
Gels

Keywords

  • Aptamer
  • Capsid
  • Chemiluminescence
  • Entropy
  • Phosphorothioate
  • Venezuelan equine encephalitis virus

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

Cite this

Combinatorial selection of a RNA thioaptamer that binds to Venezuelan equine encephalitis virus capsid protein. / Kang, Jonghoon; Lee, Myung Soog; Watowich, Stanley; Gorenstein, David G.

In: FEBS Letters, Vol. 581, No. 13, 29.05.2007, p. 2497-2502.

Research output: Contribution to journalArticle

Kang, Jonghoon ; Lee, Myung Soog ; Watowich, Stanley ; Gorenstein, David G. / Combinatorial selection of a RNA thioaptamer that binds to Venezuelan equine encephalitis virus capsid protein. In: FEBS Letters. 2007 ; Vol. 581, No. 13. pp. 2497-2502.
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