Combinatorial selection of a RNA thioaptamer that binds to Venezuelan equine encephalitis virus capsid protein

Jonghoon Kang, Myung Soog Lee, Stanley J. Watowich, David G. Gorenstein

Research output: Contribution to journalArticle

22 Scopus citations

Abstract

A phosphorothioate RNA aptamer (thioaptamer) targeting the capsid protein of Venezuelan equine encephalitis virus (VEEV) was isolated by in vitro combinatorial selection. The selected thioaptamer had a strong binding affinity (∼7 nM) and high specificity for the target protein. For the binding to the protein, the overall tertiary structure of the thioaptamer is required. We introduce two theoretical methods to examine the effect of phosphorothioate modification on the enhancement of binding affinity and one experimental method to examine the nature of the multiple bands of thioaptamer in a native gel.

Original languageEnglish (US)
Pages (from-to)2497-2502
Number of pages6
JournalFEBS Letters
Volume581
Issue number13
DOIs
StatePublished - May 29 2007

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Keywords

  • Aptamer
  • Capsid
  • Chemiluminescence
  • Entropy
  • Phosphorothioate
  • Venezuelan equine encephalitis virus

ASJC Scopus subject areas

  • Biophysics
  • Structural Biology
  • Biochemistry
  • Molecular Biology
  • Genetics
  • Cell Biology

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