Combined deficiency op myofibrillah and mitochondrial creatine kimask in diaphragm impairs power output during repetitive isotonic contractions

J. F. Watchko, J. J. Labella, M. J. Paood, A. P. Koretsky, Bill Ameredes, B. Wieringa

Research output: Contribution to journalArticle

Abstract

Creatine kinase (CK) has been implicated in the maintenance of skeletal muscle intracellular energy supply via its ATP buffering capacity. The localization of CK at the mitochondria (CK-mi) and myofibril (CK-M) is postulated to increase the efficiency of energy transduction allowing increased muscle performance. We compared the in vitro (37°) force-velocity properties of the diaphragm (DIA) from I) transgenic mice deficient in both CK-M and CK-mi (double null mutant) and 2} wild type controls (CTL) to characterize the role of CK. Optimal muscle length (Lo: 7.8±0.4 mm) and peak force at L0 (Po: 22±1 N/cm2) were not different between CTL and double null mutant DIA. Maximum velocity of shortening was lower in CKM/CK-mi deficient DIA (Vmax: 14±2 Lo/s) vs. CTL DIA (Vmax: 17±2 Lo/s) ; peak power output was obtained at 0.4 Po in both groups. The ability of CK-M/CK-mi deficient DIA to sustain shortening during repetitive isotonic contractions (75 Hz, 330 me duration repeated each sec at 0.4 Po load) was markedly impaired. CK-M/CK-mi deficient DIA velocity of shortening and power declined to zero by 27±4 sec, whereas CTL DIA power was sustained at 65% of baseline. We conclude that combined CK-M/CK-mi deficiency in DIA profoundly alters shortening capacity during repetitive ieotonic activation, underscoring the functional importance of CK in skeletal muscle.

Original languageEnglish (US)
JournalFASEB Journal
Volume10
Issue number3
StatePublished - 1996
Externally publishedYes

Fingerprint

Isotonic Contraction
creatine
Creatine
diaphragm
creatine kinase
Creatine Kinase
Diaphragms
Diaphragm
MM Form Creatine Kinase
Muscle
shortenings
Skeletal Muscle
Mitochondria
Muscles
Myofibrils
skeletal muscle
Transgenic Mice
Adenosine Triphosphate
Chemical activation
muscles

ASJC Scopus subject areas

  • Agricultural and Biological Sciences (miscellaneous)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Biochemistry
  • Cell Biology

Cite this

Combined deficiency op myofibrillah and mitochondrial creatine kimask in diaphragm impairs power output during repetitive isotonic contractions. / Watchko, J. F.; Labella, J. J.; Paood, M. J.; Koretsky, A. P.; Ameredes, Bill; Wieringa, B.

In: FASEB Journal, Vol. 10, No. 3, 1996.

Research output: Contribution to journalArticle

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AU - Watchko, J. F.

AU - Labella, J. J.

AU - Paood, M. J.

AU - Koretsky, A. P.

AU - Ameredes, Bill

AU - Wieringa, B.

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