Combined effects of hyperaminoacidemia and oxandrolone on skeletal muscle protein synthesis

Melinda Sheffield-Moore, Robert R. Wolfe, Dennis Gore, Steven Wolf, Dennis M. Ferrer, Arny A. Ferrando

Research output: Contribution to journalArticle

25 Citations (Scopus)

Abstract

We investigated whether the normal anabolic effects of acute hyperaminoacidemia were maintained after 5 days of oxandrolone (Oxandrin, Ox)-induced anabolism. Five healthy men [22 ± 3 (SD) yr] were studied before and after 5 days of oral Ox (15 mg/day). In each study, a 5-h basal period was followed by a 3-h primed-continuous infusion of a commercial amino acid mixture (10% Travasol). Stable isotopic data from blood and muscle sampling were analyzed using a three-compartment model to calculate muscle protein synthesis and breakdown. Model-derived muscle protein synthesis increased after amino acid infusion in both the control [basal control (BC) vs. control + amino acids (C + AA); P < 0.001] and Ox study [basal Ox (BOx) vs. Ox + amino acids (Ox + AA); P < 0.01], whereas protein breakdown was unchanged. Fractional synthetic rates of muscle protein increased 94% (BC vs. C + AA; P = 0.01) and 53% (BOx vs. Ox + AA; P < 0.01), respectively. We conclude that the normal anabolic effects of acute hyperaminoacidemia are maintained in skeletal muscle undergoing oxandrolone- induced anabolism.

Original languageEnglish (US)
JournalAmerican Journal of Physiology - Endocrinology and Metabolism
Volume278
Issue number2 41-2
StatePublished - Feb 2000

Fingerprint

Oxandrolone
Muscle Proteins
Skeletal Muscle
Amino Acids
Anabolic Agents
Muscle
Blood
Sampling
Muscles

Keywords

  • Amino acids
  • Anabolic agent
  • Stable isotopes

ASJC Scopus subject areas

  • Physiology
  • Endocrinology
  • Biochemistry
  • Physiology (medical)

Cite this

Combined effects of hyperaminoacidemia and oxandrolone on skeletal muscle protein synthesis. / Sheffield-Moore, Melinda; Wolfe, Robert R.; Gore, Dennis; Wolf, Steven; Ferrer, Dennis M.; Ferrando, Arny A.

In: American Journal of Physiology - Endocrinology and Metabolism, Vol. 278, No. 2 41-2, 02.2000.

Research output: Contribution to journalArticle

Sheffield-Moore, Melinda ; Wolfe, Robert R. ; Gore, Dennis ; Wolf, Steven ; Ferrer, Dennis M. ; Ferrando, Arny A. / Combined effects of hyperaminoacidemia and oxandrolone on skeletal muscle protein synthesis. In: American Journal of Physiology - Endocrinology and Metabolism. 2000 ; Vol. 278, No. 2 41-2.
@article{2b42ec0b75a54b188835b95d6a702c9d,
title = "Combined effects of hyperaminoacidemia and oxandrolone on skeletal muscle protein synthesis",
abstract = "We investigated whether the normal anabolic effects of acute hyperaminoacidemia were maintained after 5 days of oxandrolone (Oxandrin, Ox)-induced anabolism. Five healthy men [22 ± 3 (SD) yr] were studied before and after 5 days of oral Ox (15 mg/day). In each study, a 5-h basal period was followed by a 3-h primed-continuous infusion of a commercial amino acid mixture (10{\%} Travasol). Stable isotopic data from blood and muscle sampling were analyzed using a three-compartment model to calculate muscle protein synthesis and breakdown. Model-derived muscle protein synthesis increased after amino acid infusion in both the control [basal control (BC) vs. control + amino acids (C + AA); P < 0.001] and Ox study [basal Ox (BOx) vs. Ox + amino acids (Ox + AA); P < 0.01], whereas protein breakdown was unchanged. Fractional synthetic rates of muscle protein increased 94{\%} (BC vs. C + AA; P = 0.01) and 53{\%} (BOx vs. Ox + AA; P < 0.01), respectively. We conclude that the normal anabolic effects of acute hyperaminoacidemia are maintained in skeletal muscle undergoing oxandrolone- induced anabolism.",
keywords = "Amino acids, Anabolic agent, Stable isotopes",
author = "Melinda Sheffield-Moore and Wolfe, {Robert R.} and Dennis Gore and Steven Wolf and Ferrer, {Dennis M.} and Ferrando, {Arny A.}",
year = "2000",
month = "2",
language = "English (US)",
volume = "278",
journal = "American Journal of Physiology - Endocrinology and Metabolism",
issn = "0193-1849",
publisher = "American Physiological Society",
number = "2 41-2",

}

TY - JOUR

T1 - Combined effects of hyperaminoacidemia and oxandrolone on skeletal muscle protein synthesis

AU - Sheffield-Moore, Melinda

AU - Wolfe, Robert R.

AU - Gore, Dennis

AU - Wolf, Steven

AU - Ferrer, Dennis M.

AU - Ferrando, Arny A.

PY - 2000/2

Y1 - 2000/2

N2 - We investigated whether the normal anabolic effects of acute hyperaminoacidemia were maintained after 5 days of oxandrolone (Oxandrin, Ox)-induced anabolism. Five healthy men [22 ± 3 (SD) yr] were studied before and after 5 days of oral Ox (15 mg/day). In each study, a 5-h basal period was followed by a 3-h primed-continuous infusion of a commercial amino acid mixture (10% Travasol). Stable isotopic data from blood and muscle sampling were analyzed using a three-compartment model to calculate muscle protein synthesis and breakdown. Model-derived muscle protein synthesis increased after amino acid infusion in both the control [basal control (BC) vs. control + amino acids (C + AA); P < 0.001] and Ox study [basal Ox (BOx) vs. Ox + amino acids (Ox + AA); P < 0.01], whereas protein breakdown was unchanged. Fractional synthetic rates of muscle protein increased 94% (BC vs. C + AA; P = 0.01) and 53% (BOx vs. Ox + AA; P < 0.01), respectively. We conclude that the normal anabolic effects of acute hyperaminoacidemia are maintained in skeletal muscle undergoing oxandrolone- induced anabolism.

AB - We investigated whether the normal anabolic effects of acute hyperaminoacidemia were maintained after 5 days of oxandrolone (Oxandrin, Ox)-induced anabolism. Five healthy men [22 ± 3 (SD) yr] were studied before and after 5 days of oral Ox (15 mg/day). In each study, a 5-h basal period was followed by a 3-h primed-continuous infusion of a commercial amino acid mixture (10% Travasol). Stable isotopic data from blood and muscle sampling were analyzed using a three-compartment model to calculate muscle protein synthesis and breakdown. Model-derived muscle protein synthesis increased after amino acid infusion in both the control [basal control (BC) vs. control + amino acids (C + AA); P < 0.001] and Ox study [basal Ox (BOx) vs. Ox + amino acids (Ox + AA); P < 0.01], whereas protein breakdown was unchanged. Fractional synthetic rates of muscle protein increased 94% (BC vs. C + AA; P = 0.01) and 53% (BOx vs. Ox + AA; P < 0.01), respectively. We conclude that the normal anabolic effects of acute hyperaminoacidemia are maintained in skeletal muscle undergoing oxandrolone- induced anabolism.

KW - Amino acids

KW - Anabolic agent

KW - Stable isotopes

UR - http://www.scopus.com/inward/record.url?scp=0034091919&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0034091919&partnerID=8YFLogxK

M3 - Article

C2 - 10662711

AN - SCOPUS:0034091919

VL - 278

JO - American Journal of Physiology - Endocrinology and Metabolism

JF - American Journal of Physiology - Endocrinology and Metabolism

SN - 0193-1849

IS - 2 41-2

ER -