Combined Pretrauma Scopolamine and Phencyclidine Attenuate Posttraumatic Increased Sensitivity to Delayed Secondary Ischemia

L. W. Jenkins, B. G. Lyeth, K. Moszynski, G. L. Clifton, H. F. Young, R. L. Hayes, W. Lewelt, R. L. Balster, D. S. Dewitt, L. P. Miller

Research output: Contribution to journalArticle

71 Scopus citations

Abstract

Fasted Wistar rats were given a mild level of traumatic brain injury (TBI) and then subjected to 6 min of transient forebrain ischemia 24 h posttrauma. One group was given simultaneous 1 mg/kg scopolamine and 4 mg/kg phencyclidine intraperitoneally (IP) 15 min before trauma and another group an equal volume of plasmalyte A solution. After 7 days of postinjury survival, placebo-treated rats demonstrated increased posttraumatic vulnerability to secondary ischemic CA1 neuronal death even 24 h after trauma. This finding confirmed that increased posttraumatic ischemic vulnerability persists for at least 24 h even following mild trauma. Combined muscarinic receptor and N-methyl-daspartate (NMDA) receptor coupled ion channel blockade given and present during the mild TBI statistically attenuated this enhanced secondary ischemic CA1 neuronal death and thus posttraumatic increased ischemic vulnerability. Placebo-treated rats had 335.3 ± 93.6 CA1 neurons/106μm2 and drug-treated rats had 844.8 ± 184.9 CA1 neurons/106μm2. This result suggests that muscarinic and/or NMDA receptor-mediated events confined to TBI and the early posttraumatic period are in part responsible for the phenomenon of increased posttraumatic ischemic vulnerability.

Original languageEnglish (US)
Pages (from-to)275-287
Number of pages13
JournalJournal of neurotrauma
Volume5
Issue number4
DOIs
StatePublished - Jan 1 1988
Externally publishedYes

ASJC Scopus subject areas

  • Clinical Neurology

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    Jenkins, L. W., Lyeth, B. G., Moszynski, K., Clifton, G. L., Young, H. F., Hayes, R. L., Lewelt, W., Balster, R. L., Dewitt, D. S., & Miller, L. P. (1988). Combined Pretrauma Scopolamine and Phencyclidine Attenuate Posttraumatic Increased Sensitivity to Delayed Secondary Ischemia. Journal of neurotrauma, 5(4), 275-287. https://doi.org/10.1089/neu.1988.5.275