Combined treatment with vitamin C, hydrocortisone and thiamine does not attenuate morbidity and mortality of septic sheep

Tuvshintugs Baljinnyam, Satoshi Fukuda, Yosuke Niimi, Donald Prough, Perenlei Enkhbaatar

Research output: Contribution to journalArticlepeer-review

Abstract

Background: Sepsis is associated with a highest mortality rate in the ICU. Present study tests the efficacy of combined therapy with vitamin C, hydrocortisone and thiamine (combined therapy) in the ovine model of sepsis induced by Pseudomonas aeruginosa. In this study, sepsis was induced in sheep by instillation of Pseudomonas aeruginosa (1 × 1011 CFU) into the lungs via bronchoscope, under anesthesia. Nine hours after injury, intravenous infusion of vitamin C (0.75 g every 6 h), hydrocortisone (25 mg every 6 h), and thiamine (100 mg every 12 h) or saline was given to the treatment and control groups. Cardiopulmonary variables were recorded. Results: The survival rate was 16.7% in control and 33.3% in treatment groups. In the control group, mean arterial pressure dropped from 93.6 ± 8.6 to 75.5 ± 9.7 mmHg by 9 h, which was not affected by the combined therapy. Pulmonary dysfunction was not attenuated by the combined therapy either. The combined therapy had no effect on increased extravascular lung water content and fluid effusion into thoracic cavity. The bacterial number in the bronchoalveolar lavage fluid was significantly increased in the treatment group than the control group. The blood bacterial number remained comparable between groups. Conclusions: Combined vitamin C, hydrocortisone, and thiamine did not attenuate severity of ovine sepsis.

Original languageEnglish (US)
Article number27
JournalLaboratory Animal Research
Volume40
Issue number1
DOIs
StatePublished - Dec 2024

Keywords

  • Bacterial clearance
  • Hydrocortisone
  • Sepsis
  • Thiamine
  • Vitamin C

ASJC Scopus subject areas

  • General Biochemistry, Genetics and Molecular Biology
  • General Veterinary

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