Combined use of proton-proton overhauser enhancements and a distance geometry algorithm for determination of polypeptide conformations. Application to micelle-bound glucagon

Werner Braun, Chris Bösch, Larry R. Brown, Nobuhiro GO, Kurt Wüthrich

Research output: Contribution to journalArticle

219 Citations (Scopus)

Abstract

In a new approach for the determination of polypeptide conformation, experimental data on intramolecular distances between pairs of hydrogen atoms obtained from nuclear Overhauser enhancement studies are used as input for a distance geometry algorithm. The algorithm determines the limits of the conformation space occupied by the polypeptide chain. The experimental data are used in such a way that the real conformation should in all cases be within these limits. Two important features of the method are that the results do not depend critically on the accuracy of the distance measurements by nuclear Overhauser enhancement studies and that internal mobility of the polypeptide conformation is explicitly taken into consideration. The use of this new procedure is illustrated with a structural study of the region 19-27 of glucagon bound to perdeuterated dodecylphosphocholine micelles.

Original languageEnglish (US)
Pages (from-to)377-396
Number of pages20
JournalBBA - Protein Structure
Volume667
Issue number2
DOIs
StatePublished - Feb 27 1981
Externally publishedYes

Fingerprint

Micelles
Glucagon
Conformations
Protons
Peptides
Geometry
Distance measurement
Hydrogen
Atoms

Keywords

  • H-NMR
  • Distance geometry algorithm
  • Glucagon
  • Nuclear Overhauser enhancement
  • Polypeptide conformation

ASJC Scopus subject areas

  • Medicine(all)

Cite this

Combined use of proton-proton overhauser enhancements and a distance geometry algorithm for determination of polypeptide conformations. Application to micelle-bound glucagon. / Braun, Werner; Bösch, Chris; Brown, Larry R.; GO, Nobuhiro; Wüthrich, Kurt.

In: BBA - Protein Structure, Vol. 667, No. 2, 27.02.1981, p. 377-396.

Research output: Contribution to journalArticle

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AU - Wüthrich, Kurt

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