Commensal A4 bacteria inhibit intestinal Th2-cell responses through induction of dendritic cell TGF-β production

Wei Wu, Hou Pu Liu, Feidi Chen, Han Liu, Anthony T. Cao, Suxia Yao, Mingming Sun, Heather L. Evans-Marin, Ye Zhao, Qing Zhao, L. Wayne Duck, Charles O. Elson, Zhanju Liu, Yingzi Cong

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

It has been shown that while commensal bacteria promote Th1, Th17 and Treg cells in lamina propria (LP) in steady-state conditions, they suppress mucosal Th2 cells. However, it is still unclear whether there are specific commensal organisms down-regulating Th2 responses, and the mechanism involved. Here we demonstrate that commensal A4 bacteria, a member of the Lachnospiraceae family, which produce an immunodominant microbiota CBir1 antigen, inhibits LP Th2-cell development. When transferred into the intestines of RAG-/- mice, CBir1-specific T cells developed predominately towards Th1 cells and Th17 cells, but to a lesser extent into Th2 cells. The addition of A4 bacterial lysates to CD4+ T-cell cultures inhibited production of IL-4. A4 bacteria stimulated dendritic cell production of TGF-β, and blockade of TGF-β abrogated A4 bacteria inhibition of Th2-cell development in vitro and in vivo. Collectively, our data show that A4 bacteria inhibit Th2-cell differentiation by inducing dendritic cell production of TGF-β.

Original languageEnglish (US)
JournalEuropean Journal of Immunology
DOIs
StateAccepted/In press - 2016

Fingerprint

Th2 Cells
Dendritic Cells
Bacteria
Th17 Cells
Th1 Cells
Mucous Membrane
T-Lymphocytes
Microbiota
Regulatory T-Lymphocytes
Interleukin-4
Intestines
Cell Differentiation
Cell Culture Techniques
Antigens

Keywords

  • Commensal A4 bacteria
  • Dendritic cell
  • TGF-β
  • Th2

ASJC Scopus subject areas

  • Immunology
  • Immunology and Allergy

Cite this

Commensal A4 bacteria inhibit intestinal Th2-cell responses through induction of dendritic cell TGF-β production. / Wu, Wei; Liu, Hou Pu; Chen, Feidi; Liu, Han; Cao, Anthony T.; Yao, Suxia; Sun, Mingming; Evans-Marin, Heather L.; Zhao, Ye; Zhao, Qing; Duck, L. Wayne; Elson, Charles O.; Liu, Zhanju; Cong, Yingzi.

In: European Journal of Immunology, 2016.

Research output: Contribution to journalArticle

Wu, Wei ; Liu, Hou Pu ; Chen, Feidi ; Liu, Han ; Cao, Anthony T. ; Yao, Suxia ; Sun, Mingming ; Evans-Marin, Heather L. ; Zhao, Ye ; Zhao, Qing ; Duck, L. Wayne ; Elson, Charles O. ; Liu, Zhanju ; Cong, Yingzi. / Commensal A4 bacteria inhibit intestinal Th2-cell responses through induction of dendritic cell TGF-β production. In: European Journal of Immunology. 2016.
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AU - Yao, Suxia

AU - Sun, Mingming

AU - Evans-Marin, Heather L.

AU - Zhao, Ye

AU - Zhao, Qing

AU - Duck, L. Wayne

AU - Elson, Charles O.

AU - Liu, Zhanju

AU - Cong, Yingzi

PY - 2016

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