Commensal A4 bacteria inhibit intestinal Th2-cell responses through induction of dendritic cell TGF-β production

Wei Wu, Hou Pu Liu, Feidi Chen, Han Liu, Anthony T. Cao, Suxia Yao, Mingming Sun, Heather L. Evans-Marin, Ye Zhao, Qing Zhao, L. Wayne Duck, Charles O. Elson, Zhanju Liu, Yingzi Cong

Research output: Contribution to journalArticle

18 Scopus citations

Abstract

It has been shown that while commensal bacteria promote Th1, Th17 and Treg cells in lamina propria (LP) in steady-state conditions, they suppress mucosal Th2 cells. However, it is still unclear whether there are specific commensal organisms down-regulating Th2 responses, and the mechanism involved. Here we demonstrate that commensal A4 bacteria, a member of the Lachnospiraceae family, which produce an immunodominant microbiota CBir1 antigen, inhibits LP Th2-cell development. When transferred into the intestines of RAG-/- mice, CBir1-specific T cells developed predominately towards Th1 cells and Th17 cells, but to a lesser extent into Th2 cells. The addition of A4 bacterial lysates to CD4+ T-cell cultures inhibited production of IL-4. A4 bacteria stimulated dendritic cell production of TGF-β, and blockade of TGF-β abrogated A4 bacteria inhibition of Th2-cell development in vitro and in vivo. Collectively, our data show that A4 bacteria inhibit Th2-cell differentiation by inducing dendritic cell production of TGF-β.

Original languageEnglish (US)
JournalEuropean Journal of Immunology
DOIs
StateAccepted/In press - 2016

Keywords

  • Commensal A4 bacteria
  • Dendritic cell
  • TGF-β
  • Th2

ASJC Scopus subject areas

  • Immunology
  • Immunology and Allergy

Fingerprint Dive into the research topics of 'Commensal A4 bacteria inhibit intestinal Th2-cell responses through induction of dendritic cell TGF-β production'. Together they form a unique fingerprint.

  • Cite this

    Wu, W., Liu, H. P., Chen, F., Liu, H., Cao, A. T., Yao, S., Sun, M., Evans-Marin, H. L., Zhao, Y., Zhao, Q., Duck, L. W., Elson, C. O., Liu, Z., & Cong, Y. (Accepted/In press). Commensal A4 bacteria inhibit intestinal Th2-cell responses through induction of dendritic cell TGF-β production. European Journal of Immunology. https://doi.org/10.1002/eji.201546160