Commensal bacteria modulate innate immune responses of vaginal epithelial cell multilayer cultures

William A. Rose, Chris L. McGowin, Rae Ann Spagnuolo, Tonyia Eaves-Pyles, Vsevolod Popov, Richard Pyles

Research output: Contribution to journalArticle

53 Citations (Scopus)

Abstract

The human vaginal microbiome plays a critical but poorly defined role in reproductive health. Vaginal microbiome alterations are associated with increased susceptibility to sexually-transmitted infections (STI) possibly due to related changes in innate defense responses from epithelial cells. Study of the impact of commensal bacteria on the vaginal mucosal surface has been hindered by current vaginal epithelial cell (VEC) culture systems that lack an appropriate interface between the apical surface of stratified squamous epithelium and the air-filled vaginal lumen. Therefore we developed a reproducible multilayer VEC culture system with an apical (luminal) air-interface that supported colonization with selected commensal bacteria. Multilayer VEC developed tight-junctions and other hallmarks of the vaginal mucosa including predictable proinflammatory cytokine secretion following TLR stimulation. Colonization of multilayers by common vaginal commensals including Lactobacillus crispatus, L. jensenii, and L. rhamnosus led to intimate associations with the VEC exclusively on the apical surface. Vaginal commensals did not trigger cytokine secretion but Staphylococcus epidermidis, a skin commensal, was inflammatory. Lactobacilli reduced cytokine secretion in an isolate-specific fashion following TLR stimulation. This tempering of inflammation offers a potential explanation for increased susceptibility to STI in the absence of common commensals and has implications for testing of potential STI preventatives.

Original languageEnglish (US)
Article numbere32728
JournalPLoS One
Volume7
Issue number3
DOIs
StatePublished - Mar 7 2012

Fingerprint

Innate Immunity
Bacteria
Multilayers
cell culture
epithelial cells
sexually transmitted diseases
Cell Culture Techniques
Epithelial Cells
Sexually Transmitted Diseases
bacteria
cytokines
Microbiota
secretion
Cytokines
Cell culture
Lactobacillus jensenii
Lactobacillus crispatus
vaginal mucosa
Air
tempering

ASJC Scopus subject areas

  • Agricultural and Biological Sciences(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Medicine(all)

Cite this

Commensal bacteria modulate innate immune responses of vaginal epithelial cell multilayer cultures. / Rose, William A.; McGowin, Chris L.; Spagnuolo, Rae Ann; Eaves-Pyles, Tonyia; Popov, Vsevolod; Pyles, Richard.

In: PLoS One, Vol. 7, No. 3, e32728, 07.03.2012.

Research output: Contribution to journalArticle

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