Comparative analyses of transcriptional profiles in mouse organs using a pneumonic plague model after infection with wild-type Yersinia pestis CO92 and its Braun lipoprotein mutant

Ashok K. Chopra, Cristi L. Galindo, Scott T. Moen, Elena V. Kozlova, Jian Sha, Harold R. Garner, Stacy L. Agar

Research output: Contribution to journalArticle

11 Scopus citations

Abstract

We employed Murine GeneChips to delineate the global transcriptional profiles of the livers, lungs, and spleens in a mouse pneumonic plague infection model with wild-type (WT) Y. pestis CO92 and its Braun lipoprotein (Δ l p p) mutant with reduced virulence. These organs showed differential transcriptional responses to infection with WT Y. pestis, but the overall host functional processes affected were similar across all three tissues. Gene expression alterations were found in inflammation, cytokine signaling, and apoptotic cell death-associated genes. Comparison of WT and Δ l p p mutant-infected mice indicated significant overlap in lipopolysaccharide- (LPS-) associated gene expression, but the absence of Lpp perturbed host cell signaling at critical regulatory junctions resulting in altered immune response and possibly host cell apoptosis. We generated a putative signaling pathway including major inflammatory components that could account for the synergistic action of LPS and Lpp and provided the mechanistic basis of attenuation caused by deletion of the lpp gene from Y. pestis in a mouse model of pneumonic plague.

Original languageEnglish (US)
Article number914762
JournalComparative and Functional Genomics
Volume2009
DOIs
StatePublished - 2009

ASJC Scopus subject areas

  • Biotechnology
  • Molecular Biology
  • Genetics

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