Comparative antigenic analysis of spotted fever group rickettsiae from Israel and other closely related organisms

David Walker, H. M. Feng, J. I. Saada, P. Crocquet-Valdes, S. Radulovic, Vsevolod Popov, E. Manor

Research output: Contribution to journalArticle

35 Citations (Scopus)

Abstract

Spotted fever rickettsiosis in Israel has been considered as possibly somewhat more severe than boutonneuse fever, from which it also differs in having a very low proportion of cases with a tick-inoculation site eschar. This investigation was undertaken to determine whether the Israeli spotted fever group (SFG) rickettsiae differed sufficiently from Rickettsia conorii to be considered as a distinct species. Strains of Rickettsia conorii from Morocco and South Africa, four SFG rickettsial isolates from Israel, one from Russia, and one from Zimbabwe were compared by microimmunofluorescence serotyping, Western immunoblotting, monoclonal antibody reactivity, and polymerase chain reaction amplification of the repeat domain of the rickettsial outer membrane protein A (rOmpA). All are strains and isolates of R. conorii, yet there is considerable molecular and antigenic diversity of both rOmpA and rickettsial outer membrane protein B (rOmpB) among them. The rOmpA gene of the Israeli isolates and the Astrakhan strain from Russia is estimated to encode 15 rOmpA repeat units as compared with 10 for the South African strain and six for the strains from Morocco and Zimbabwe. The Israeli SFG rickettsial strains appear to be R. conorii, a species with substantial antigenic and genetic diversity. The Israeli strains appear to fall within the limit previously described for the genetic and antigenic diversity of R. conorii.

Original languageEnglish (US)
Pages (from-to)569-576
Number of pages8
JournalAmerican Journal of Tropical Medicine and Hygiene
Volume52
Issue number6
StatePublished - 1995

Fingerprint

Rickettsia conorii
Rickettsia
Israel
Fever
Antigenic Variation
Zimbabwe
Morocco
Russia
Boutonneuse Fever
Serotyping
Ticks
South Africa
Membrane Proteins
Western Blotting
Monoclonal Antibodies
Polymerase Chain Reaction
Rickettsia rOmpA
Genes

ASJC Scopus subject areas

  • Parasitology
  • Infectious Diseases

Cite this

Comparative antigenic analysis of spotted fever group rickettsiae from Israel and other closely related organisms. / Walker, David; Feng, H. M.; Saada, J. I.; Crocquet-Valdes, P.; Radulovic, S.; Popov, Vsevolod; Manor, E.

In: American Journal of Tropical Medicine and Hygiene, Vol. 52, No. 6, 1995, p. 569-576.

Research output: Contribution to journalArticle

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