Comparative Burkholderia pseudomallei natural history virulence studies using an aerosol murine model of infection

Shane Massey, Linsey A. Yeager, Carla A. Blumentritt, Sudhamathi Vijayakumar, Elena Sbrana, Johnny Peterson, Trevor Brasel, James LeDuc, Janice Endsley, Alfredo Torres

Research output: Contribution to journalArticle

22 Citations (Scopus)

Abstract

Melioidosis is an endemic disease caused by the bacterium Burkholderia pseudomallei. Concerns exist regarding B. pseudomallei use as a potential bio-threat agent causing persistent infections and typically manifesting as severe pneumonia capable of causing fatal bacteremia. Development of suitable therapeutics against melioidosis is complicated due to high degree of genetic and phenotypic variability among B. pseudomallei isolates and lack of data establishing commonly accepted strains for comparative studies. Further, the impact of strain variation on virulence, disease presentation, and mortality is not well understood. Therefore, this study evaluate and compare the virulence and disease progression of B. pseudomallei strains K96243 and HBPUB10303a, following aerosol challenge in a standardized BALB/c mouse model of infection. The natural history analysis of disease progression monitored conditions such as weight, body temperature, appearance, activity, bacteremia, organ and tissue colonization (pathological and histological analysis) and immunological responses. This study provides a detailed, direct comparison of infection with different B. pseudomallei strains and set up the basis for a standardized model useful to test different medical countermeasures against Burkholderia species. Further, this protocol serves as a guideline to standardize other bacterial aerosol models of infection or to define biomarkers of infectious processes caused by other intracellular pathogens.

Original languageEnglish (US)
Article number4305
JournalScientific Reports
Volume4
DOIs
StatePublished - Mar 7 2014

Fingerprint

Burkholderia pseudomallei
Natural History
Aerosols
Virulence
Melioidosis
Infection
Bacteremia
Disease Progression
Burkholderia
Endemic Diseases
Body Temperature
Pneumonia
Biomarkers
Guidelines
Bacteria
Weights and Measures
Mortality

ASJC Scopus subject areas

  • General

Cite this

Comparative Burkholderia pseudomallei natural history virulence studies using an aerosol murine model of infection. / Massey, Shane; Yeager, Linsey A.; Blumentritt, Carla A.; Vijayakumar, Sudhamathi; Sbrana, Elena; Peterson, Johnny; Brasel, Trevor; LeDuc, James; Endsley, Janice; Torres, Alfredo.

In: Scientific Reports, Vol. 4, 4305, 07.03.2014.

Research output: Contribution to journalArticle

Massey, Shane ; Yeager, Linsey A. ; Blumentritt, Carla A. ; Vijayakumar, Sudhamathi ; Sbrana, Elena ; Peterson, Johnny ; Brasel, Trevor ; LeDuc, James ; Endsley, Janice ; Torres, Alfredo. / Comparative Burkholderia pseudomallei natural history virulence studies using an aerosol murine model of infection. In: Scientific Reports. 2014 ; Vol. 4.
@article{216a3b3b1cf844219095a0754f6f6c4e,
title = "Comparative Burkholderia pseudomallei natural history virulence studies using an aerosol murine model of infection",
abstract = "Melioidosis is an endemic disease caused by the bacterium Burkholderia pseudomallei. Concerns exist regarding B. pseudomallei use as a potential bio-threat agent causing persistent infections and typically manifesting as severe pneumonia capable of causing fatal bacteremia. Development of suitable therapeutics against melioidosis is complicated due to high degree of genetic and phenotypic variability among B. pseudomallei isolates and lack of data establishing commonly accepted strains for comparative studies. Further, the impact of strain variation on virulence, disease presentation, and mortality is not well understood. Therefore, this study evaluate and compare the virulence and disease progression of B. pseudomallei strains K96243 and HBPUB10303a, following aerosol challenge in a standardized BALB/c mouse model of infection. The natural history analysis of disease progression monitored conditions such as weight, body temperature, appearance, activity, bacteremia, organ and tissue colonization (pathological and histological analysis) and immunological responses. This study provides a detailed, direct comparison of infection with different B. pseudomallei strains and set up the basis for a standardized model useful to test different medical countermeasures against Burkholderia species. Further, this protocol serves as a guideline to standardize other bacterial aerosol models of infection or to define biomarkers of infectious processes caused by other intracellular pathogens.",
author = "Shane Massey and Yeager, {Linsey A.} and Blumentritt, {Carla A.} and Sudhamathi Vijayakumar and Elena Sbrana and Johnny Peterson and Trevor Brasel and James LeDuc and Janice Endsley and Alfredo Torres",
year = "2014",
month = "3",
day = "7",
doi = "10.1038/srep04305",
language = "English (US)",
volume = "4",
journal = "Scientific Reports",
issn = "2045-2322",
publisher = "Nature Publishing Group",

}

TY - JOUR

T1 - Comparative Burkholderia pseudomallei natural history virulence studies using an aerosol murine model of infection

AU - Massey, Shane

AU - Yeager, Linsey A.

AU - Blumentritt, Carla A.

AU - Vijayakumar, Sudhamathi

AU - Sbrana, Elena

AU - Peterson, Johnny

AU - Brasel, Trevor

AU - LeDuc, James

AU - Endsley, Janice

AU - Torres, Alfredo

PY - 2014/3/7

Y1 - 2014/3/7

N2 - Melioidosis is an endemic disease caused by the bacterium Burkholderia pseudomallei. Concerns exist regarding B. pseudomallei use as a potential bio-threat agent causing persistent infections and typically manifesting as severe pneumonia capable of causing fatal bacteremia. Development of suitable therapeutics against melioidosis is complicated due to high degree of genetic and phenotypic variability among B. pseudomallei isolates and lack of data establishing commonly accepted strains for comparative studies. Further, the impact of strain variation on virulence, disease presentation, and mortality is not well understood. Therefore, this study evaluate and compare the virulence and disease progression of B. pseudomallei strains K96243 and HBPUB10303a, following aerosol challenge in a standardized BALB/c mouse model of infection. The natural history analysis of disease progression monitored conditions such as weight, body temperature, appearance, activity, bacteremia, organ and tissue colonization (pathological and histological analysis) and immunological responses. This study provides a detailed, direct comparison of infection with different B. pseudomallei strains and set up the basis for a standardized model useful to test different medical countermeasures against Burkholderia species. Further, this protocol serves as a guideline to standardize other bacterial aerosol models of infection or to define biomarkers of infectious processes caused by other intracellular pathogens.

AB - Melioidosis is an endemic disease caused by the bacterium Burkholderia pseudomallei. Concerns exist regarding B. pseudomallei use as a potential bio-threat agent causing persistent infections and typically manifesting as severe pneumonia capable of causing fatal bacteremia. Development of suitable therapeutics against melioidosis is complicated due to high degree of genetic and phenotypic variability among B. pseudomallei isolates and lack of data establishing commonly accepted strains for comparative studies. Further, the impact of strain variation on virulence, disease presentation, and mortality is not well understood. Therefore, this study evaluate and compare the virulence and disease progression of B. pseudomallei strains K96243 and HBPUB10303a, following aerosol challenge in a standardized BALB/c mouse model of infection. The natural history analysis of disease progression monitored conditions such as weight, body temperature, appearance, activity, bacteremia, organ and tissue colonization (pathological and histological analysis) and immunological responses. This study provides a detailed, direct comparison of infection with different B. pseudomallei strains and set up the basis for a standardized model useful to test different medical countermeasures against Burkholderia species. Further, this protocol serves as a guideline to standardize other bacterial aerosol models of infection or to define biomarkers of infectious processes caused by other intracellular pathogens.

UR - http://www.scopus.com/inward/record.url?scp=84896752656&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84896752656&partnerID=8YFLogxK

U2 - 10.1038/srep04305

DO - 10.1038/srep04305

M3 - Article

C2 - 24603493

AN - SCOPUS:84896752656

VL - 4

JO - Scientific Reports

JF - Scientific Reports

SN - 2045-2322

M1 - 4305

ER -