Comparative diagnosis of neonatal chlamydial conjunctivitis by polymerase chain reaction and McCoy cell culture

A. R. Talley, F. Garcia-Ferrer, K. A. Laycock, L. R. Essary, W. L. Holcomb, B. E. Flowers, M. Loeffelholz, C. Lewinski, J. S. Pepose

Research output: Contribution to journalArticle

18 Citations (Scopus)

Abstract

Cervical and ocular swabs from 100 mother/newborn pairs delivering on the clinic service were assayed for Chlamydia trachomatis with standard McCoy cell culture and with standard and biotinylated polymerase chain reaction techniques, using primers directed against the major outer membrane protein gene and C. trachomatis-specific cryptic plasmid, respectively. Using the polymerase chain reaction, 20 (20%) mothers and seven (7%) neonates were positive for Chlamydia. All neonates positive by polymerase chain reaction were from mothers positive by polymerase chain reaction, yielding a 35% transmission rate. Only five of 20 (25%) mothers and two of seven (28%) neonates positive by polymerase chain reaction were positive by cell culture. All cell culture samples were positive by polymerase chain reaction testing. Culture and polymerase chain reaction analysis two weeks after treatment with oral erythromycin were negative. The polymerase chain reaction assay appears to be equally specific and more sensitive than McCoy cell culture for the detection of C. trachomatis from ocular specimens.

Original languageEnglish (US)
Pages (from-to)50-57
Number of pages8
JournalAmerican Journal of Ophthalmology
Volume117
Issue number1
StatePublished - 1994
Externally publishedYes

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Conjunctivitis
Cell Culture Techniques
Polymerase Chain Reaction
Chlamydia trachomatis
Chlamydia
Erythromycin
Plasmids

ASJC Scopus subject areas

  • Ophthalmology

Cite this

Talley, A. R., Garcia-Ferrer, F., Laycock, K. A., Essary, L. R., Holcomb, W. L., Flowers, B. E., ... Pepose, J. S. (1994). Comparative diagnosis of neonatal chlamydial conjunctivitis by polymerase chain reaction and McCoy cell culture. American Journal of Ophthalmology, 117(1), 50-57.

Comparative diagnosis of neonatal chlamydial conjunctivitis by polymerase chain reaction and McCoy cell culture. / Talley, A. R.; Garcia-Ferrer, F.; Laycock, K. A.; Essary, L. R.; Holcomb, W. L.; Flowers, B. E.; Loeffelholz, M.; Lewinski, C.; Pepose, J. S.

In: American Journal of Ophthalmology, Vol. 117, No. 1, 1994, p. 50-57.

Research output: Contribution to journalArticle

Talley, AR, Garcia-Ferrer, F, Laycock, KA, Essary, LR, Holcomb, WL, Flowers, BE, Loeffelholz, M, Lewinski, C & Pepose, JS 1994, 'Comparative diagnosis of neonatal chlamydial conjunctivitis by polymerase chain reaction and McCoy cell culture', American Journal of Ophthalmology, vol. 117, no. 1, pp. 50-57.
Talley AR, Garcia-Ferrer F, Laycock KA, Essary LR, Holcomb WL, Flowers BE et al. Comparative diagnosis of neonatal chlamydial conjunctivitis by polymerase chain reaction and McCoy cell culture. American Journal of Ophthalmology. 1994;117(1):50-57.
Talley, A. R. ; Garcia-Ferrer, F. ; Laycock, K. A. ; Essary, L. R. ; Holcomb, W. L. ; Flowers, B. E. ; Loeffelholz, M. ; Lewinski, C. ; Pepose, J. S. / Comparative diagnosis of neonatal chlamydial conjunctivitis by polymerase chain reaction and McCoy cell culture. In: American Journal of Ophthalmology. 1994 ; Vol. 117, No. 1. pp. 50-57.
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AU - Garcia-Ferrer, F.

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AU - Essary, L. R.

AU - Holcomb, W. L.

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