Comparative effect of recombinant IL-1, -2, -3, -4, and -6, IFN-γ, granulocyte-macrophage-colony-stimulating factor, tumor necrosis factor-α, and histamine-releasing factors on the secretion of histamine from basophils

R. Alam, J. B. Welter, P. A. Forsythe, M. A. Lett-Brown, J. A. Grant

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Abstract

Most cytokines possess multiple biologic activities. This study was undertaken to investigate the effect of rIL-1β, -2, -3, -4 and -6, IFN-γ, TNF-α, and granulocyte-macrophage (GM)-CSF on basophils from 16 donors and the amount of histamine releases was compared with that by partially purified mononuclear cell-derived histamine-releasing factor (HRF) and anti-IgE. We found that only IL-3 and GM-CSF at relatively high doses (50 to 500 ng/ml) released small amounts of histamine (3 to 14%) from two allergic donors. In contrast, both HRF and anti-IgE released significant amounts of histamine from all donors. Other cytokines did not release any measurable quantity of histamine. Simultaneous addition of several cytokines to the basophils also failed to release histamine. IL-3, GM-CSF, and IL-1 can also release histamine at lower concentrations (< 5 ng/ml) when incubated with basophils in the presence of D2O. Basophils from 6 out of 13 allergic donors released histamine in response to IL-3, whereas three donors responded to IL-1β and two responded to GM-CSF. The results of this study demonstrated that although IL-3 and GM-CSF release small amounts of histamine only from a select group of allergic patients, mononuclear cell-derived HRF is more potent in their action and release histamine from normals as well as allergic patients.

Original languageEnglish (US)
Pages (from-to)3431-3435
Number of pages5
JournalJournal of Immunology
Volume142
Issue number10
StatePublished - 1989

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Basophils
Granulocyte-Macrophage Colony-Stimulating Factor
Interleukin-1
Granulocytes
Histamine
Histamine Release
Interleukin-3
Interleukin-2
Tumor Necrosis Factor-alpha
Macrophages
Tissue Donors
Cytokines
Macrophage Colony-Stimulating Factor
translationally-controlled 1 tumor protein

ASJC Scopus subject areas

  • Immunology

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Comparative effect of recombinant IL-1, -2, -3, -4, and -6, IFN-γ, granulocyte-macrophage-colony-stimulating factor, tumor necrosis factor-α, and histamine-releasing factors on the secretion of histamine from basophils. / Alam, R.; Welter, J. B.; Forsythe, P. A.; Lett-Brown, M. A.; Grant, J. A.

In: Journal of Immunology, Vol. 142, No. 10, 1989, p. 3431-3435.

Research output: Contribution to journalArticle

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abstract = "Most cytokines possess multiple biologic activities. This study was undertaken to investigate the effect of rIL-1β, -2, -3, -4 and -6, IFN-γ, TNF-α, and granulocyte-macrophage (GM)-CSF on basophils from 16 donors and the amount of histamine releases was compared with that by partially purified mononuclear cell-derived histamine-releasing factor (HRF) and anti-IgE. We found that only IL-3 and GM-CSF at relatively high doses (50 to 500 ng/ml) released small amounts of histamine (3 to 14{\%}) from two allergic donors. In contrast, both HRF and anti-IgE released significant amounts of histamine from all donors. Other cytokines did not release any measurable quantity of histamine. Simultaneous addition of several cytokines to the basophils also failed to release histamine. IL-3, GM-CSF, and IL-1 can also release histamine at lower concentrations (< 5 ng/ml) when incubated with basophils in the presence of D2O. Basophils from 6 out of 13 allergic donors released histamine in response to IL-3, whereas three donors responded to IL-1β and two responded to GM-CSF. The results of this study demonstrated that although IL-3 and GM-CSF release small amounts of histamine only from a select group of allergic patients, mononuclear cell-derived HRF is more potent in their action and release histamine from normals as well as allergic patients.",
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