Comparative effects on rat primary astrocytes and C6 rat glioma cells cultures after 24-h exposure to silver nanoparticles (AgNPs)

Samuel Salazar-García, Ana Sonia Silva-Ramírez, Manuel A. Ramirez-Lee, Hector Rosas-Hernandez, Edgar Rangel-López, Claudia G. Castillo, Abel Santamaría, Gabriel A. Martinez-Castañon, Carmen Gonzalez

Research output: Contribution to journalArticlepeer-review

13 Scopus citations


The aim of this work was to compare the effects of 24-h exposure of rat primary astrocytes and C6 rat glioma cells to 7.8 nm AgNPs. Glioblastoma multiforme (GBM) is the most aggressive primary brain tumor and current treatments lead to diverse side-effects; for this reason, it is imperative to investigate new approaches, including those alternatives provided by nanotechnology, like nanomaterials (NMs) such as silver nanoparticles. Herein, we found that C6 rat glioma cells, but no primary astrocytes, decreased cell viability after AgNPs treatment; however, both cell types diminished their proliferation. The decrease of glioma C6 cells proliferation was related with necrosis, while in primary astrocytes, the decreased proliferation was associated with the induction of apoptosis. The ionic control (AgNO3) exerted a different profile than AgNPs; the bulk form did not modify the basal effect in each determination, whereas cisplatin, a well-known antitumoral drug used as a comparative control, promoted cytotoxicity in both cell types at specific concentrations. Our findings prompt the need to determine the fine molecular and cellular mechanisms involved in the differential biological responses to AgNPs in order to develop new tools or alternatives based on nanotechnology that may contribute to the understanding, impact and use of NMs in specific targets, like glioblastoma cells.

Original languageEnglish (US)
Article number450
Pages (from-to)1-13
Number of pages13
JournalJournal of Nanoparticle Research
Issue number11
StatePublished - Nov 1 2015
Externally publishedYes


  • Apoptosis
  • Cytotoxicity
  • Glioma
  • Primary astrocytes
  • Proliferation
  • Silver nanoparticles

ASJC Scopus subject areas

  • Bioengineering
  • General Chemistry
  • Atomic and Molecular Physics, and Optics
  • Modeling and Simulation
  • General Materials Science
  • Condensed Matter Physics


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