Comparative electrophoretic analysis of the virus proteins of four rhabdoviruses

J. F. Obijeski, A. T. Marchenko, D. H L Bishop, B. W. Cann, F. A. Murphy

Research output: Contribution to journalArticle

69 Citations (Scopus)

Abstract

The structural proteins of vesicular stomatitis virus (VSV) Indiana, VSV New Jersey, Chandipura virus annd Piry virus were compared by using two different SDS polyacrylamide gel electrophoresis systems. The G and M proteins of the Indiana serotype had different electrophoretic mobilities from the corresponding proteins of VSV New Jersey, Chandipura virus and Piry virus in a continuous SDS (CONT SDS) gel system. The three major proteins of VSV Indiana G, N and M, were distinctly different from their counterpart proteins in the other three viruses when a high resolution discontinuous SDS (DISC SDS) system was used. Chandipura and Piry virus glycoproteins migrated differently when co electrophoresed in the CONT SDS system, whereas both their N and M proteins were different in the DISC SDS method. During infection both Chandipura and Piry viruses produced defective T particles which contained the same four structural proteins as virus particles.

Original languageEnglish (US)
Pages (from-to)21-33
Number of pages13
JournalJournal of General Virology
Volume22
Issue number1
StatePublished - 1974

Fingerprint

Rhabdoviridae
Vesiculovirus
Viruses
Vesicular stomatitis New Jersey virus
Vesicular stomatitis Indiana virus
Proteins
Virion
Polyacrylamide Gel Electrophoresis
Glycoproteins
Gels

ASJC Scopus subject areas

  • Virology
  • Immunology

Cite this

Obijeski, J. F., Marchenko, A. T., Bishop, D. H. L., Cann, B. W., & Murphy, F. A. (1974). Comparative electrophoretic analysis of the virus proteins of four rhabdoviruses. Journal of General Virology, 22(1), 21-33.

Comparative electrophoretic analysis of the virus proteins of four rhabdoviruses. / Obijeski, J. F.; Marchenko, A. T.; Bishop, D. H L; Cann, B. W.; Murphy, F. A.

In: Journal of General Virology, Vol. 22, No. 1, 1974, p. 21-33.

Research output: Contribution to journalArticle

Obijeski, JF, Marchenko, AT, Bishop, DHL, Cann, BW & Murphy, FA 1974, 'Comparative electrophoretic analysis of the virus proteins of four rhabdoviruses', Journal of General Virology, vol. 22, no. 1, pp. 21-33.
Obijeski JF, Marchenko AT, Bishop DHL, Cann BW, Murphy FA. Comparative electrophoretic analysis of the virus proteins of four rhabdoviruses. Journal of General Virology. 1974;22(1):21-33.
Obijeski, J. F. ; Marchenko, A. T. ; Bishop, D. H L ; Cann, B. W. ; Murphy, F. A. / Comparative electrophoretic analysis of the virus proteins of four rhabdoviruses. In: Journal of General Virology. 1974 ; Vol. 22, No. 1. pp. 21-33.
@article{bdccd1e76fd946508ddd5a124c8b8368,
title = "Comparative electrophoretic analysis of the virus proteins of four rhabdoviruses",
abstract = "The structural proteins of vesicular stomatitis virus (VSV) Indiana, VSV New Jersey, Chandipura virus annd Piry virus were compared by using two different SDS polyacrylamide gel electrophoresis systems. The G and M proteins of the Indiana serotype had different electrophoretic mobilities from the corresponding proteins of VSV New Jersey, Chandipura virus and Piry virus in a continuous SDS (CONT SDS) gel system. The three major proteins of VSV Indiana G, N and M, were distinctly different from their counterpart proteins in the other three viruses when a high resolution discontinuous SDS (DISC SDS) system was used. Chandipura and Piry virus glycoproteins migrated differently when co electrophoresed in the CONT SDS system, whereas both their N and M proteins were different in the DISC SDS method. During infection both Chandipura and Piry viruses produced defective T particles which contained the same four structural proteins as virus particles.",
author = "Obijeski, {J. F.} and Marchenko, {A. T.} and Bishop, {D. H L} and Cann, {B. W.} and Murphy, {F. A.}",
year = "1974",
language = "English (US)",
volume = "22",
pages = "21--33",
journal = "Journal of General Virology",
issn = "0022-1317",
publisher = "Society for General Microbiology",
number = "1",

}

TY - JOUR

T1 - Comparative electrophoretic analysis of the virus proteins of four rhabdoviruses

AU - Obijeski, J. F.

AU - Marchenko, A. T.

AU - Bishop, D. H L

AU - Cann, B. W.

AU - Murphy, F. A.

PY - 1974

Y1 - 1974

N2 - The structural proteins of vesicular stomatitis virus (VSV) Indiana, VSV New Jersey, Chandipura virus annd Piry virus were compared by using two different SDS polyacrylamide gel electrophoresis systems. The G and M proteins of the Indiana serotype had different electrophoretic mobilities from the corresponding proteins of VSV New Jersey, Chandipura virus and Piry virus in a continuous SDS (CONT SDS) gel system. The three major proteins of VSV Indiana G, N and M, were distinctly different from their counterpart proteins in the other three viruses when a high resolution discontinuous SDS (DISC SDS) system was used. Chandipura and Piry virus glycoproteins migrated differently when co electrophoresed in the CONT SDS system, whereas both their N and M proteins were different in the DISC SDS method. During infection both Chandipura and Piry viruses produced defective T particles which contained the same four structural proteins as virus particles.

AB - The structural proteins of vesicular stomatitis virus (VSV) Indiana, VSV New Jersey, Chandipura virus annd Piry virus were compared by using two different SDS polyacrylamide gel electrophoresis systems. The G and M proteins of the Indiana serotype had different electrophoretic mobilities from the corresponding proteins of VSV New Jersey, Chandipura virus and Piry virus in a continuous SDS (CONT SDS) gel system. The three major proteins of VSV Indiana G, N and M, were distinctly different from their counterpart proteins in the other three viruses when a high resolution discontinuous SDS (DISC SDS) system was used. Chandipura and Piry virus glycoproteins migrated differently when co electrophoresed in the CONT SDS system, whereas both their N and M proteins were different in the DISC SDS method. During infection both Chandipura and Piry viruses produced defective T particles which contained the same four structural proteins as virus particles.

UR - http://www.scopus.com/inward/record.url?scp=0015951208&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0015951208&partnerID=8YFLogxK

M3 - Article

C2 - 4360393

AN - SCOPUS:0015951208

VL - 22

SP - 21

EP - 33

JO - Journal of General Virology

JF - Journal of General Virology

SN - 0022-1317

IS - 1

ER -