Comparative genome- and proteome analysis of cerebral cortex from MK-801 treated rats

Linda Paulson, Peter Martin, Anders Persson, Carol L. Nilsson, Elisabeth Ljung, Ann Westman-Brinkmalm, Peter S. Eriksson, Kaj Blennow, Pia Davidsson

Research output: Contribution to journalArticle

83 Scopus citations

Abstract

cDNA microarrays and two-dimensional gel-electrophoresis in combination with mass spectrometry, were used to screen alterations in mRNA and protein levels, respectively, in cerebral cortex of MK-801-treated rats. The rats were divided in two groups; group 1 (short-term treated) and group 2 (long-term treated). In group 1, four genes were up-regulated and five down-regulated. In group 2, seven genes were up-regulated and six down-regulated. In group 1, the levels of one protein was increased and eight proteins reduced. In group 2, the levels of two proteins were increased and four proteins reduced. Several of the altered genes (casein kinase 2, glutamic acid decarboxylase, synaptotagmin, gamma aminobutyric acid [GABA] transporter, creatine kinase, and cytochrome c oxidase) and proteins (superoxide dismutase, hsp 60, hsp 72 and γ-enolase) have previously been connected to schizophrenia. Alterations of the genes (microglobulin, c-jun proto-oncogene, 40S ribosomal protein S19, adenosine diphosphate (ADP)ribosylation factors, platelet-derived growth factor, fructose-bisphophate aldolase A, and myelin proteolipid) and the proteins (stathmin, H+-transp. Adenosine triphosphate (ATP) synthase, pyruvate dehydrogenase, β-actin and α-enolase), have not, to our knowledge, earlier been implicated in schizophrenia pathology. Overall, these results with a combined approach of genomics and proteomics add to the validity of subchronic N-methylD-aspartate (NMDA)-receptor antagonist treatment as an animal model of schizophrenia.

Original languageEnglish (US)
Pages (from-to)526-533
Number of pages8
JournalJournal of Neuroscience Research
Volume71
Issue number4
DOIs
StatePublished - Feb 15 2003

Keywords

  • Brain
  • MK-801
  • Rat
  • Two-dimensional gel electrophoresis
  • cDNA microarray

ASJC Scopus subject areas

  • Cellular and Molecular Neuroscience

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    Paulson, L., Martin, P., Persson, A., Nilsson, C. L., Ljung, E., Westman-Brinkmalm, A., Eriksson, P. S., Blennow, K., & Davidsson, P. (2003). Comparative genome- and proteome analysis of cerebral cortex from MK-801 treated rats. Journal of Neuroscience Research, 71(4), 526-533. https://doi.org/10.1002/jnr.10509