Comparative global gene expression profiles of wild-type yersinia pestis CO92 and its braun lipoprotein mutant at flea and human body temperatures

Cristi L. Galindo, Jian Sha, Scott T. Moen, Stacy L. Agar, Michelle L. Kirtley, Sheri M. Foltz, Lauren J. McIver, E. V. Kozlova, Harold R. Garner, Ashok Chopra

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

Braun/murein lipoprotein (Lpp) is involved in inflammatory responses and septic shock. We previously characterized a Δlpp mutant of Yersinia pestis CO92 and found that this mutant was defective in surviving in macrophages and was attenuated in a mouse inhalation model of plague when compared to the highly virulent wild-type (WT) bacterium. We performed global transcriptional profiling of WT Y. pestis and its Δlpp mutant using microarrays. The organisms were cultured at 26 and 37 degrees Celsius to simulate the flea vector and mammalian host environments, respectively. Our data revealed vastly different effects of lpp mutation on the transcriptomes of Y. pestis grown at 37 versus 26 C °. While the absence of Lpp resulted mainly in the downregulation of metabolic genes at 26 C °, the Y. pestisΔlpp mutant cultured at 37 C °exhibited profound alterations in stress response and virulence genes, compared to WT bacteria. We investigated one of the stress-related genes (htrA) downregulated in the Δlpp mutant relative to WT Y. pestis. Indeed, complementation of the Δlpp mutant with the htrA gene restored intracellular survival of the Y. pestisΔlpp mutant. Our results support a role for Lpp in Y. pestis adaptation to the host environment, possibly via transcriptional activation of htrA.

Original languageEnglish (US)
Article number342168
JournalComparative and Functional Genomics
Volume2010
DOIs
StatePublished - 2010

Fingerprint

Yersinia pestis
Siphonaptera
Body Temperature
Human Body
Transcriptome
Lipoproteins
Genes
Down-Regulation
Bacteria
Plague
Peptidoglycan
Septic Shock
Inhalation
Transcriptional Activation
Virulence
Macrophages
Mutation

ASJC Scopus subject areas

  • Genetics
  • Molecular Biology
  • Biotechnology

Cite this

Comparative global gene expression profiles of wild-type yersinia pestis CO92 and its braun lipoprotein mutant at flea and human body temperatures. / Galindo, Cristi L.; Sha, Jian; Moen, Scott T.; Agar, Stacy L.; Kirtley, Michelle L.; Foltz, Sheri M.; McIver, Lauren J.; Kozlova, E. V.; Garner, Harold R.; Chopra, Ashok.

In: Comparative and Functional Genomics, Vol. 2010, 342168, 2010.

Research output: Contribution to journalArticle

Galindo, Cristi L. ; Sha, Jian ; Moen, Scott T. ; Agar, Stacy L. ; Kirtley, Michelle L. ; Foltz, Sheri M. ; McIver, Lauren J. ; Kozlova, E. V. ; Garner, Harold R. ; Chopra, Ashok. / Comparative global gene expression profiles of wild-type yersinia pestis CO92 and its braun lipoprotein mutant at flea and human body temperatures. In: Comparative and Functional Genomics. 2010 ; Vol. 2010.
@article{f1303cde02834bd2a91d0d410de88390,
title = "Comparative global gene expression profiles of wild-type yersinia pestis CO92 and its braun lipoprotein mutant at flea and human body temperatures",
abstract = "Braun/murein lipoprotein (Lpp) is involved in inflammatory responses and septic shock. We previously characterized a Δlpp mutant of Yersinia pestis CO92 and found that this mutant was defective in surviving in macrophages and was attenuated in a mouse inhalation model of plague when compared to the highly virulent wild-type (WT) bacterium. We performed global transcriptional profiling of WT Y. pestis and its Δlpp mutant using microarrays. The organisms were cultured at 26 and 37 degrees Celsius to simulate the flea vector and mammalian host environments, respectively. Our data revealed vastly different effects of lpp mutation on the transcriptomes of Y. pestis grown at 37 versus 26 C °. While the absence of Lpp resulted mainly in the downregulation of metabolic genes at 26 C °, the Y. pestisΔlpp mutant cultured at 37 C °exhibited profound alterations in stress response and virulence genes, compared to WT bacteria. We investigated one of the stress-related genes (htrA) downregulated in the Δlpp mutant relative to WT Y. pestis. Indeed, complementation of the Δlpp mutant with the htrA gene restored intracellular survival of the Y. pestisΔlpp mutant. Our results support a role for Lpp in Y. pestis adaptation to the host environment, possibly via transcriptional activation of htrA.",
author = "Galindo, {Cristi L.} and Jian Sha and Moen, {Scott T.} and Agar, {Stacy L.} and Kirtley, {Michelle L.} and Foltz, {Sheri M.} and McIver, {Lauren J.} and Kozlova, {E. V.} and Garner, {Harold R.} and Ashok Chopra",
year = "2010",
doi = "10.1155/2010/342168",
language = "English (US)",
volume = "2010",
journal = "Comparative and Functional Genomics",
issn = "1531-6912",
publisher = "Hindawi Publishing Corporation",

}

TY - JOUR

T1 - Comparative global gene expression profiles of wild-type yersinia pestis CO92 and its braun lipoprotein mutant at flea and human body temperatures

AU - Galindo, Cristi L.

AU - Sha, Jian

AU - Moen, Scott T.

AU - Agar, Stacy L.

AU - Kirtley, Michelle L.

AU - Foltz, Sheri M.

AU - McIver, Lauren J.

AU - Kozlova, E. V.

AU - Garner, Harold R.

AU - Chopra, Ashok

PY - 2010

Y1 - 2010

N2 - Braun/murein lipoprotein (Lpp) is involved in inflammatory responses and septic shock. We previously characterized a Δlpp mutant of Yersinia pestis CO92 and found that this mutant was defective in surviving in macrophages and was attenuated in a mouse inhalation model of plague when compared to the highly virulent wild-type (WT) bacterium. We performed global transcriptional profiling of WT Y. pestis and its Δlpp mutant using microarrays. The organisms were cultured at 26 and 37 degrees Celsius to simulate the flea vector and mammalian host environments, respectively. Our data revealed vastly different effects of lpp mutation on the transcriptomes of Y. pestis grown at 37 versus 26 C °. While the absence of Lpp resulted mainly in the downregulation of metabolic genes at 26 C °, the Y. pestisΔlpp mutant cultured at 37 C °exhibited profound alterations in stress response and virulence genes, compared to WT bacteria. We investigated one of the stress-related genes (htrA) downregulated in the Δlpp mutant relative to WT Y. pestis. Indeed, complementation of the Δlpp mutant with the htrA gene restored intracellular survival of the Y. pestisΔlpp mutant. Our results support a role for Lpp in Y. pestis adaptation to the host environment, possibly via transcriptional activation of htrA.

AB - Braun/murein lipoprotein (Lpp) is involved in inflammatory responses and septic shock. We previously characterized a Δlpp mutant of Yersinia pestis CO92 and found that this mutant was defective in surviving in macrophages and was attenuated in a mouse inhalation model of plague when compared to the highly virulent wild-type (WT) bacterium. We performed global transcriptional profiling of WT Y. pestis and its Δlpp mutant using microarrays. The organisms were cultured at 26 and 37 degrees Celsius to simulate the flea vector and mammalian host environments, respectively. Our data revealed vastly different effects of lpp mutation on the transcriptomes of Y. pestis grown at 37 versus 26 C °. While the absence of Lpp resulted mainly in the downregulation of metabolic genes at 26 C °, the Y. pestisΔlpp mutant cultured at 37 C °exhibited profound alterations in stress response and virulence genes, compared to WT bacteria. We investigated one of the stress-related genes (htrA) downregulated in the Δlpp mutant relative to WT Y. pestis. Indeed, complementation of the Δlpp mutant with the htrA gene restored intracellular survival of the Y. pestisΔlpp mutant. Our results support a role for Lpp in Y. pestis adaptation to the host environment, possibly via transcriptional activation of htrA.

UR - http://www.scopus.com/inward/record.url?scp=77953492910&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=77953492910&partnerID=8YFLogxK

U2 - 10.1155/2010/342168

DO - 10.1155/2010/342168

M3 - Article

VL - 2010

JO - Comparative and Functional Genomics

JF - Comparative and Functional Genomics

SN - 1531-6912

M1 - 342168

ER -