Comparative peptidomics of four related hemipteran species

Pyrokinins, myosuppressin, corazonin, adipokinetic hormone, sNPF, and periviscerokinins

Reinhard Predel, William Russell, David H. Russell, Juan Lopez, Jesus Esquivel, Ronald J. Nachman

Research output: Contribution to journalArticle

37 Citations (Scopus)

Abstract

We performed the first comprehensive peptidomic analysis of neurohormones from hemipteran insects by analyzing the neuropeptides of two major neurohemal organs, namely the corpora cardiaca and abdominal perisympathetic organs. For the experiments we selected four related species of polyphagous stinkbugs (Pentatomidae), three of which are known to attack several important food crops. Peptide sequences were identified by MALDI-TOF mass spectrometry; tandem fragmentation of myosuppressin, sNPF, CAPA-periviscerokinins and pyrokinins revealed novel sequences not known from other insects so far. Most Leu/Ile and Glu/Lys ambiguities could be solved by either specific side-chain fragmentations or on-plate acetylation experiments. The identification of the specific sequences provides a solid basis for forthcoming pharmacological tests to study the neuroendocrine system of these pest insects. However, it should be mentioned in this context that the sequences of the peptides from different stinkbugs are likely not representative of Hemiptera in general. The forthcoming release of the genome from the reduviid Rhodnius prolixus will provide sufficient data to clear this point.

Original languageEnglish (US)
Pages (from-to)162-167
Number of pages6
JournalPeptides
Volume29
Issue number2
DOIs
StatePublished - Feb 2008
Externally publishedYes

Fingerprint

Insects
Acetylation
Peptides
Neuropeptides
Corpora Allata
Rhodnius
Crops
Mass spectrometry
Neurotransmitter Agents
Hemiptera
Neurosecretory Systems
Genes
Experiments
Matrix-Assisted Laser Desorption-Ionization Mass Spectrometry
Mass Spectrometry
Genome
Pharmacology
Food
periviscerokinin
pyrokinin

Keywords

  • Acrosternum hilare
  • Banasa dimiata
  • CAPA-peptides
  • Euschistus servus
  • FXPRLamide
  • Insect neuropeptides
  • Mass spectrometry
  • Nezara viridula
  • Pentatomidae

ASJC Scopus subject areas

  • Biochemistry
  • Endocrinology
  • Physiology
  • Cellular and Molecular Neuroscience

Cite this

Comparative peptidomics of four related hemipteran species : Pyrokinins, myosuppressin, corazonin, adipokinetic hormone, sNPF, and periviscerokinins. / Predel, Reinhard; Russell, William; Russell, David H.; Lopez, Juan; Esquivel, Jesus; Nachman, Ronald J.

In: Peptides, Vol. 29, No. 2, 02.2008, p. 162-167.

Research output: Contribution to journalArticle

Predel, Reinhard ; Russell, William ; Russell, David H. ; Lopez, Juan ; Esquivel, Jesus ; Nachman, Ronald J. / Comparative peptidomics of four related hemipteran species : Pyrokinins, myosuppressin, corazonin, adipokinetic hormone, sNPF, and periviscerokinins. In: Peptides. 2008 ; Vol. 29, No. 2. pp. 162-167.
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