Comparative proteome analysis of thalamus in MK-801-treated rats

Linda Paulson, Peter Martin, Carol L. Nilsson, Elisabeth Ljung, Ann Westman-Brinkmalm, Kaj Blennow, Pia Davidsson

Research output: Contribution to journalArticle

43 Citations (Scopus)

Abstract

Two-dimensional gel-electrophoresis in combination with mass spectrometry is a powerful approach to compare protein expression in brain tissues. Using this proteomic approach, and based on the hypothesis that schizophrenia involves hypoglutamergic brain function, alterations in protein levels in the thalamus of rats treated with the N-methyl-D-aspartate (NMDA) receptor antagonist [+]-5-methyl-10,11-dihydro-5H-dibenzo-[a,d]-cycloheptene-5, 10-iminehydrogenmaleate (MK-801), as compared to saline-treated animals, were assessed in an unbiased fashion. The rats were divided into two groups; group 1 (short-term treated) and group 2 (long-term treated). In group 1, the levels of seven proteins were increased and four proteins reduced. In group 2, the levels of six proteins were reduced. Several of the altered proteins (heat shock proteins 60 and 72, albumin, dihydropyrimidinase related protein-2, aldolase c, and malate dehydrogenase) have previously been connected to schizophrenia. Alterations of other proteins (dihydrolipoamide acetyltransferase component of pyruvate dehydrogenase complex E2, guanine deaminase, α-enolase, aconitase, ATP-synthase and α-internexin), have not, to the best of our knowledge, earlier been implicated in schizophrenia pathology. Our results show the high potential of using proteomic methods for the validation of animal models of schizophrenia and to identify new proteins involved in the pathophysiological mechanisms of schizophrenia.

Original languageEnglish (US)
Pages (from-to)819-825
Number of pages7
JournalProteomics
Volume4
Issue number3
DOIs
StatePublished - Mar 2004
Externally publishedYes

Fingerprint

Dizocilpine Maleate
Proteome
Thalamus
Rats
Schizophrenia
Dihydrolipoyllysine-Residue Acetyltransferase
Proteins
Brain
Proteomics
Animals
Guanine Deaminase
HSP72 Heat-Shock Proteins
Aconitate Hydratase
Chaperonin 60
Malate Dehydrogenase
Fructose-Bisphosphate Aldolase
Phosphopyruvate Hydratase
Pathology
N-Methyl-D-Aspartate Receptors
Electrophoresis

Keywords

  • Mass spectrometry
  • Rat
  • Thalamus
  • Two-dimensional gel electrophoresis

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

Cite this

Paulson, L., Martin, P., Nilsson, C. L., Ljung, E., Westman-Brinkmalm, A., Blennow, K., & Davidsson, P. (2004). Comparative proteome analysis of thalamus in MK-801-treated rats. Proteomics, 4(3), 819-825. https://doi.org/10.1002/pmic.200300622

Comparative proteome analysis of thalamus in MK-801-treated rats. / Paulson, Linda; Martin, Peter; Nilsson, Carol L.; Ljung, Elisabeth; Westman-Brinkmalm, Ann; Blennow, Kaj; Davidsson, Pia.

In: Proteomics, Vol. 4, No. 3, 03.2004, p. 819-825.

Research output: Contribution to journalArticle

Paulson, L, Martin, P, Nilsson, CL, Ljung, E, Westman-Brinkmalm, A, Blennow, K & Davidsson, P 2004, 'Comparative proteome analysis of thalamus in MK-801-treated rats', Proteomics, vol. 4, no. 3, pp. 819-825. https://doi.org/10.1002/pmic.200300622
Paulson L, Martin P, Nilsson CL, Ljung E, Westman-Brinkmalm A, Blennow K et al. Comparative proteome analysis of thalamus in MK-801-treated rats. Proteomics. 2004 Mar;4(3):819-825. https://doi.org/10.1002/pmic.200300622
Paulson, Linda ; Martin, Peter ; Nilsson, Carol L. ; Ljung, Elisabeth ; Westman-Brinkmalm, Ann ; Blennow, Kaj ; Davidsson, Pia. / Comparative proteome analysis of thalamus in MK-801-treated rats. In: Proteomics. 2004 ; Vol. 4, No. 3. pp. 819-825.
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