Comparative salivary gland transcriptomics of sandfly vectors of visceral leishmaniasis

Jennifer M. Anderson, Fabiano Oliveira, Shaden Kamhawi, Ben J. Mans, David Reynoso, Amy E. Seitz, Phillip Lawyer, Mark Garfield, My Van Pham, Jesus G. Valenzuela

Research output: Contribution to journalArticle

110 Citations (Scopus)

Abstract

Background: Immune responses to sandfly saliva have been shown to protect animals against Leishmania infection. Yet very little is known about the molecular characteristics of salivary proteins from different sandflies, particularly from vectors transmitting visceral leishmaniasis, the fatal form of the disease. Further knowledge of the repertoire of these salivary proteins will give us insights into the molecular evolution of these proteins and will help us select relevant antigens for the development of a vector based anti-Leishmania vaccine. Results: Two salivary gland cDNA libraries from female sandflies Phlebotomus argentipes and P. perniciosus were constructed, sequenced and proteomic analysis of the salivary proteins was performed. The majority of the sequenced transcripts from the two cDNA libraries coded for secreted proteins. In this analysis we identified transcripts coding for protein families not previously described in sandflies. A comparative sandfly salivary transcriptome analysis was performed by using these two cDNA libraries and two other sandfly salivary gland cDNA libraries from P. ariasi and Lutzomyia longipalpis, also vectors of visceral leishmaniasis. Full-length secreted proteins from each sandfly library were compared using a stand-alone version of BLAST, creating formatted protein databases of each sandfly library. Related groups of proteins from each sandfly species were combined into defined families of proteins. With this comparison, we identified families of salivary proteins common among all of the sandflies studied, proteins to be genus specific and proteins that appear to be species specific. The common proteins included apyrase, yellow-related protein, antigen-5, PpSP15 and PpSP32-related protein, a 33-kDa protein, D7-related protein, a 39- and a 16.1 - kDa protein and an endonuclease-like protein. Some of these families contained multiple members, including PPSP15-like, yellow proteins and D7-related proteins suggesting gene expansion in these proteins. Conclusion: This comprehensive analysis allows us the identification of genus- specific proteins, species-specific proteins and, more importantly, proteins common among these different sandflies. These results give us insights into the repertoire of salivary proteins that are potential candidates for a vector-based vaccine.

Original languageEnglish (US)
Article number52
JournalBMC Genomics
Volume7
DOIs
StatePublished - Mar 15 2006
Externally publishedYes

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Psychodidae
Visceral Leishmaniasis
Salivary Glands
Proteins
Salivary Proteins and Peptides
Gene Library
Libraries
Leishmaniasis Vaccines
Phlebotomus
Apyrase

ASJC Scopus subject areas

  • Biotechnology
  • Genetics

Cite this

Anderson, J. M., Oliveira, F., Kamhawi, S., Mans, B. J., Reynoso, D., Seitz, A. E., ... Valenzuela, J. G. (2006). Comparative salivary gland transcriptomics of sandfly vectors of visceral leishmaniasis. BMC Genomics, 7, [52]. https://doi.org/10.1186/1471-2164-7-52

Comparative salivary gland transcriptomics of sandfly vectors of visceral leishmaniasis. / Anderson, Jennifer M.; Oliveira, Fabiano; Kamhawi, Shaden; Mans, Ben J.; Reynoso, David; Seitz, Amy E.; Lawyer, Phillip; Garfield, Mark; Pham, My Van; Valenzuela, Jesus G.

In: BMC Genomics, Vol. 7, 52, 15.03.2006.

Research output: Contribution to journalArticle

Anderson, JM, Oliveira, F, Kamhawi, S, Mans, BJ, Reynoso, D, Seitz, AE, Lawyer, P, Garfield, M, Pham, MV & Valenzuela, JG 2006, 'Comparative salivary gland transcriptomics of sandfly vectors of visceral leishmaniasis', BMC Genomics, vol. 7, 52. https://doi.org/10.1186/1471-2164-7-52
Anderson, Jennifer M. ; Oliveira, Fabiano ; Kamhawi, Shaden ; Mans, Ben J. ; Reynoso, David ; Seitz, Amy E. ; Lawyer, Phillip ; Garfield, Mark ; Pham, My Van ; Valenzuela, Jesus G. / Comparative salivary gland transcriptomics of sandfly vectors of visceral leishmaniasis. In: BMC Genomics. 2006 ; Vol. 7.
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