Comparative sensitivity of the VecTest antigen-capture assay, reverse transcriptase-PCR, and cell culture for detection of West Nile virus in dead birds

Marina Siirin, Chris Sargent, Rebecca C. Langer, Ray Parsons, Dana L. Vanlandingham, Stephen Higgs, Robert B. Tesh

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

The sensitivity of the VecTest antigen-capture and RT-PCR assays were compared to brain tissue culture in Vero cells for the detection of WNV in a sample of dead birds collected in East Texas and southern Louisiana during the summer of 2003. Cell culture was used as the gold standard, because it yielded the most positives. Direct culture of brain tissue and the WNV antigen-capture assay, done on oropharyngeal swabs, gave statistically comparable results (46% and 40% positive, respectively). In contrast, RT-PCR done on brain homogenates was significantly less sensitive than direct tissue culture (33% compared to 46%, respectively). Results indicated that RT-PCR may not be consistently the most sensitive assay for detection of WNV in dead bird brain tissue and that the VecTest antigen-capture assay has a number of advantages over the other two detection techniques for routine surveillance activities.

Original languageEnglish (US)
Pages (from-to)204-209
Number of pages6
JournalVector-Borne and Zoonotic Diseases
Volume4
Issue number3
DOIs
StatePublished - Sep 2004

Fingerprint

West Nile virus
Reverse Transcriptase Polymerase Chain Reaction
Birds
Cell Culture Techniques
Antigens
Polymerase Chain Reaction
Brain
Vero Cells
Brain Death

Keywords

  • Arbovirus
  • Virus assay
  • West Nile virus

ASJC Scopus subject areas

  • Infectious Diseases
  • Parasitology

Cite this

Comparative sensitivity of the VecTest antigen-capture assay, reverse transcriptase-PCR, and cell culture for detection of West Nile virus in dead birds. / Siirin, Marina; Sargent, Chris; Langer, Rebecca C.; Parsons, Ray; Vanlandingham, Dana L.; Higgs, Stephen; Tesh, Robert B.

In: Vector-Borne and Zoonotic Diseases, Vol. 4, No. 3, 09.2004, p. 204-209.

Research output: Contribution to journalArticle

Siirin, Marina ; Sargent, Chris ; Langer, Rebecca C. ; Parsons, Ray ; Vanlandingham, Dana L. ; Higgs, Stephen ; Tesh, Robert B. / Comparative sensitivity of the VecTest antigen-capture assay, reverse transcriptase-PCR, and cell culture for detection of West Nile virus in dead birds. In: Vector-Borne and Zoonotic Diseases. 2004 ; Vol. 4, No. 3. pp. 204-209.
@article{8750356f8d464ec099a1968277455ed5,
title = "Comparative sensitivity of the VecTest antigen-capture assay, reverse transcriptase-PCR, and cell culture for detection of West Nile virus in dead birds",
abstract = "The sensitivity of the VecTest antigen-capture and RT-PCR assays were compared to brain tissue culture in Vero cells for the detection of WNV in a sample of dead birds collected in East Texas and southern Louisiana during the summer of 2003. Cell culture was used as the gold standard, because it yielded the most positives. Direct culture of brain tissue and the WNV antigen-capture assay, done on oropharyngeal swabs, gave statistically comparable results (46{\%} and 40{\%} positive, respectively). In contrast, RT-PCR done on brain homogenates was significantly less sensitive than direct tissue culture (33{\%} compared to 46{\%}, respectively). Results indicated that RT-PCR may not be consistently the most sensitive assay for detection of WNV in dead bird brain tissue and that the VecTest antigen-capture assay has a number of advantages over the other two detection techniques for routine surveillance activities.",
keywords = "Arbovirus, Virus assay, West Nile virus",
author = "Marina Siirin and Chris Sargent and Langer, {Rebecca C.} and Ray Parsons and Vanlandingham, {Dana L.} and Stephen Higgs and Tesh, {Robert B.}",
year = "2004",
month = "9",
doi = "10.1089/vbz.2004.4.204",
language = "English (US)",
volume = "4",
pages = "204--209",
journal = "Vector-Borne and Zoonotic Diseases",
issn = "1530-3667",
publisher = "Mary Ann Liebert Inc.",
number = "3",

}

TY - JOUR

T1 - Comparative sensitivity of the VecTest antigen-capture assay, reverse transcriptase-PCR, and cell culture for detection of West Nile virus in dead birds

AU - Siirin, Marina

AU - Sargent, Chris

AU - Langer, Rebecca C.

AU - Parsons, Ray

AU - Vanlandingham, Dana L.

AU - Higgs, Stephen

AU - Tesh, Robert B.

PY - 2004/9

Y1 - 2004/9

N2 - The sensitivity of the VecTest antigen-capture and RT-PCR assays were compared to brain tissue culture in Vero cells for the detection of WNV in a sample of dead birds collected in East Texas and southern Louisiana during the summer of 2003. Cell culture was used as the gold standard, because it yielded the most positives. Direct culture of brain tissue and the WNV antigen-capture assay, done on oropharyngeal swabs, gave statistically comparable results (46% and 40% positive, respectively). In contrast, RT-PCR done on brain homogenates was significantly less sensitive than direct tissue culture (33% compared to 46%, respectively). Results indicated that RT-PCR may not be consistently the most sensitive assay for detection of WNV in dead bird brain tissue and that the VecTest antigen-capture assay has a number of advantages over the other two detection techniques for routine surveillance activities.

AB - The sensitivity of the VecTest antigen-capture and RT-PCR assays were compared to brain tissue culture in Vero cells for the detection of WNV in a sample of dead birds collected in East Texas and southern Louisiana during the summer of 2003. Cell culture was used as the gold standard, because it yielded the most positives. Direct culture of brain tissue and the WNV antigen-capture assay, done on oropharyngeal swabs, gave statistically comparable results (46% and 40% positive, respectively). In contrast, RT-PCR done on brain homogenates was significantly less sensitive than direct tissue culture (33% compared to 46%, respectively). Results indicated that RT-PCR may not be consistently the most sensitive assay for detection of WNV in dead bird brain tissue and that the VecTest antigen-capture assay has a number of advantages over the other two detection techniques for routine surveillance activities.

KW - Arbovirus

KW - Virus assay

KW - West Nile virus

UR - http://www.scopus.com/inward/record.url?scp=7244238186&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=7244238186&partnerID=8YFLogxK

U2 - 10.1089/vbz.2004.4.204

DO - 10.1089/vbz.2004.4.204

M3 - Article

C2 - 15631064

AN - SCOPUS:7244238186

VL - 4

SP - 204

EP - 209

JO - Vector-Borne and Zoonotic Diseases

JF - Vector-Borne and Zoonotic Diseases

SN - 1530-3667

IS - 3

ER -