Comparative study of regenerative effects of mesenchymal stem cells derived from placental amnion, chorion and umbilical cord on dermal wounds

Juliane Ertl, Melanie Pichlsberger, Alexandru Cristian Tuca, Paul Wurzer, Jakob Fuchs, Stefan H. Geyer, Barbara Maurer-Gesek, Wolfgang J. Weninger, Dagmar Pfeiffer, Vladimir Bubalo, Daryousch Parvizi, Lars Peter Kamolz, Ingrid Lang

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

Objective: Mesenchymal stem/stromal cells derived from human term placentas (PMSCs) are novel therapeutic agents and more topical than ever. Here we evaluated the effects of three types of PMSCs on wound healing in an in vivo mouse model: Amnion-derived MSCs (AMSCs), blood vessel-derived MSCs (BV-MSCs) from the chorionic plate and Wharton's jelly-derived MSCs (WJ-MSCs) from the umbilical cord. Methods: We topically applied PMSCs onto skin wounds in mice using the dermal substitute Matriderm® as carrier and evaluated wound healing parameters. In addition, we investigated the effects of all PMSC types under co-application with placental endothelial cells (PLECs). After 8 days, we compared the percent of wound closure and the angiogenic potential between all groups. Results: AMSCs, BV-MSCs and WJ-MSCs significantly induced a faster healing and a higher number of blood vessels in the wound when compared to controls (Matriderm®-alone). PLECs did not further improve the advantageous effects of PMSC-treatment. Quantitative data and 3D analysis by high resolution episcopic microscopy confirmed a lower density of vessels in Matriderm®/PMSCs/PLECs co-application compared to Matriderm®/PMSCs treatment. Conclusion: Results indicate that all three PMSC types exert similar beneficial effects on wound closure and neovascularization in our mouse model. Practice: Using Matriderm® as carrier for PMSCs propagates rapid cell migration towards the wound area that allows a fast and clinically practicable method for stem cell application. Implications: These promising effects warrant further investigation in clinical trials.

Original languageEnglish (US)
Pages (from-to)37-46
Number of pages10
JournalPlacenta
Volume65
DOIs
StatePublished - May 1 2018
Externally publishedYes

Fingerprint

Chorion
Amnion
Umbilical Cord
Mesenchymal Stromal Cells
Wharton Jelly
Skin
Wounds and Injuries
Blood Vessels
Endothelial Cells
Wound Healing
Artificial Skin
Placenta
Cell Movement
Microscopy
Stem Cells
matriderm
Clinical Trials

Keywords

  • Amnion
  • Angiogenesis
  • Blood vessels
  • Human placenta
  • Mesenchymal stem cells
  • Mouse model
  • Umbilical cord
  • Wound healing

ASJC Scopus subject areas

  • Reproductive Medicine
  • Obstetrics and Gynecology
  • Developmental Biology

Cite this

Comparative study of regenerative effects of mesenchymal stem cells derived from placental amnion, chorion and umbilical cord on dermal wounds. / Ertl, Juliane; Pichlsberger, Melanie; Tuca, Alexandru Cristian; Wurzer, Paul; Fuchs, Jakob; Geyer, Stefan H.; Maurer-Gesek, Barbara; Weninger, Wolfgang J.; Pfeiffer, Dagmar; Bubalo, Vladimir; Parvizi, Daryousch; Kamolz, Lars Peter; Lang, Ingrid.

In: Placenta, Vol. 65, 01.05.2018, p. 37-46.

Research output: Contribution to journalArticle

Ertl, J, Pichlsberger, M, Tuca, AC, Wurzer, P, Fuchs, J, Geyer, SH, Maurer-Gesek, B, Weninger, WJ, Pfeiffer, D, Bubalo, V, Parvizi, D, Kamolz, LP & Lang, I 2018, 'Comparative study of regenerative effects of mesenchymal stem cells derived from placental amnion, chorion and umbilical cord on dermal wounds', Placenta, vol. 65, pp. 37-46. https://doi.org/10.1016/j.placenta.2018.04.004
Ertl, Juliane ; Pichlsberger, Melanie ; Tuca, Alexandru Cristian ; Wurzer, Paul ; Fuchs, Jakob ; Geyer, Stefan H. ; Maurer-Gesek, Barbara ; Weninger, Wolfgang J. ; Pfeiffer, Dagmar ; Bubalo, Vladimir ; Parvizi, Daryousch ; Kamolz, Lars Peter ; Lang, Ingrid. / Comparative study of regenerative effects of mesenchymal stem cells derived from placental amnion, chorion and umbilical cord on dermal wounds. In: Placenta. 2018 ; Vol. 65. pp. 37-46.
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abstract = "Objective: Mesenchymal stem/stromal cells derived from human term placentas (PMSCs) are novel therapeutic agents and more topical than ever. Here we evaluated the effects of three types of PMSCs on wound healing in an in vivo mouse model: Amnion-derived MSCs (AMSCs), blood vessel-derived MSCs (BV-MSCs) from the chorionic plate and Wharton's jelly-derived MSCs (WJ-MSCs) from the umbilical cord. Methods: We topically applied PMSCs onto skin wounds in mice using the dermal substitute Matriderm{\circledR} as carrier and evaluated wound healing parameters. In addition, we investigated the effects of all PMSC types under co-application with placental endothelial cells (PLECs). After 8 days, we compared the percent of wound closure and the angiogenic potential between all groups. Results: AMSCs, BV-MSCs and WJ-MSCs significantly induced a faster healing and a higher number of blood vessels in the wound when compared to controls (Matriderm{\circledR}-alone). PLECs did not further improve the advantageous effects of PMSC-treatment. Quantitative data and 3D analysis by high resolution episcopic microscopy confirmed a lower density of vessels in Matriderm{\circledR}/PMSCs/PLECs co-application compared to Matriderm{\circledR}/PMSCs treatment. Conclusion: Results indicate that all three PMSC types exert similar beneficial effects on wound closure and neovascularization in our mouse model. Practice: Using Matriderm{\circledR} as carrier for PMSCs propagates rapid cell migration towards the wound area that allows a fast and clinically practicable method for stem cell application. Implications: These promising effects warrant further investigation in clinical trials.",
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AU - Ertl, Juliane

AU - Pichlsberger, Melanie

AU - Tuca, Alexandru Cristian

AU - Wurzer, Paul

AU - Fuchs, Jakob

AU - Geyer, Stefan H.

AU - Maurer-Gesek, Barbara

AU - Weninger, Wolfgang J.

AU - Pfeiffer, Dagmar

AU - Bubalo, Vladimir

AU - Parvizi, Daryousch

AU - Kamolz, Lars Peter

AU - Lang, Ingrid

PY - 2018/5/1

Y1 - 2018/5/1

N2 - Objective: Mesenchymal stem/stromal cells derived from human term placentas (PMSCs) are novel therapeutic agents and more topical than ever. Here we evaluated the effects of three types of PMSCs on wound healing in an in vivo mouse model: Amnion-derived MSCs (AMSCs), blood vessel-derived MSCs (BV-MSCs) from the chorionic plate and Wharton's jelly-derived MSCs (WJ-MSCs) from the umbilical cord. Methods: We topically applied PMSCs onto skin wounds in mice using the dermal substitute Matriderm® as carrier and evaluated wound healing parameters. In addition, we investigated the effects of all PMSC types under co-application with placental endothelial cells (PLECs). After 8 days, we compared the percent of wound closure and the angiogenic potential between all groups. Results: AMSCs, BV-MSCs and WJ-MSCs significantly induced a faster healing and a higher number of blood vessels in the wound when compared to controls (Matriderm®-alone). PLECs did not further improve the advantageous effects of PMSC-treatment. Quantitative data and 3D analysis by high resolution episcopic microscopy confirmed a lower density of vessels in Matriderm®/PMSCs/PLECs co-application compared to Matriderm®/PMSCs treatment. Conclusion: Results indicate that all three PMSC types exert similar beneficial effects on wound closure and neovascularization in our mouse model. Practice: Using Matriderm® as carrier for PMSCs propagates rapid cell migration towards the wound area that allows a fast and clinically practicable method for stem cell application. Implications: These promising effects warrant further investigation in clinical trials.

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KW - Angiogenesis

KW - Blood vessels

KW - Human placenta

KW - Mesenchymal stem cells

KW - Mouse model

KW - Umbilical cord

KW - Wound healing

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