Comparative study of solutions for pulmonary preservation using an isolated rabbit lung model

Ghannam Al-Dossari, Sara J. Shumway

Research output: Contribution to journalArticle

Abstract

At the University of Minnesota, University of Wisconsin (UW), modified Euro-Collins (MEC), and Marshall (M) solutions were compared as agents for pulmonary preservation in an isolated rabbit lung model. Normal saline (NS) was used as a control. The heart-lung blocks of donor rabbits were flushed with, and then preserved in, one of the solutions at 4°C. Five rabbits were studied in each group. After 8 h of cold ischemia, the left lung was ventilated and reperfused with fresh venous blood from donor rabbits for 30 min. Pulmonary function was assessed by serial measurements of oxygen (O2) and carbon dioxide (CO2) tensions in blood obtained from the left atrial appendage. The ratios of wet/dry (W/D) weight of the lungs were calculated to assess the extent of pulmonary edema. After 8 h of preservation followed by 30 min of reperfusion, O2 tension was significantly higher with UW (178.36 + 1.72 mmHg). The calculated P values were UW vs NS, 2 tension with UW was also lower than the other solutions: UW, 35.8 ± 0.698 mmHg; NS, 48.5 ± 0.745 mmHg; MEC, 40.69 ± 0.749 mmHg; and M, 44.68 ± 0.697 mmHg. The calculated P value was UW vs NS, 0.0001; UW vs MEC, 0.0003; and UW vs M, 0.0001 using repeated-measures analysis of covariance. The W/D ratio was lower with UW as well: UW, 6.82 ± 0.19; NS, 8.01 ± 0.23; MEC, 7.28 ± 0.10; and M, 7.34 ± 0.17. The P value was

Original languageEnglish (US)
Pages (from-to)187-191
Number of pages5
JournalJournal of Surgical Research
Volume75
Issue number2
DOIs
StatePublished - Mar 1998
Externally publishedYes

Fingerprint

Rabbits
Lung
Cold Ischemia
Atrial Appendage
Heart Block
Pulmonary Edema
Blood Donors
Carbon Dioxide
Reperfusion
Tissue Donors
Oxygen
Weights and Measures

Keywords

  • Preservation
  • Pulmonary
  • Solutions

ASJC Scopus subject areas

  • Surgery

Cite this

Comparative study of solutions for pulmonary preservation using an isolated rabbit lung model. / Al-Dossari, Ghannam; Shumway, Sara J.

In: Journal of Surgical Research, Vol. 75, No. 2, 03.1998, p. 187-191.

Research output: Contribution to journalArticle

Al-Dossari, Ghannam ; Shumway, Sara J. / Comparative study of solutions for pulmonary preservation using an isolated rabbit lung model. In: Journal of Surgical Research. 1998 ; Vol. 75, No. 2. pp. 187-191.
@article{0c263f115e644a11b6b1ac6901a5ae3d,
title = "Comparative study of solutions for pulmonary preservation using an isolated rabbit lung model",
abstract = "At the University of Minnesota, University of Wisconsin (UW), modified Euro-Collins (MEC), and Marshall (M) solutions were compared as agents for pulmonary preservation in an isolated rabbit lung model. Normal saline (NS) was used as a control. The heart-lung blocks of donor rabbits were flushed with, and then preserved in, one of the solutions at 4°C. Five rabbits were studied in each group. After 8 h of cold ischemia, the left lung was ventilated and reperfused with fresh venous blood from donor rabbits for 30 min. Pulmonary function was assessed by serial measurements of oxygen (O2) and carbon dioxide (CO2) tensions in blood obtained from the left atrial appendage. The ratios of wet/dry (W/D) weight of the lungs were calculated to assess the extent of pulmonary edema. After 8 h of preservation followed by 30 min of reperfusion, O2 tension was significantly higher with UW (178.36 + 1.72 mmHg). The calculated P values were UW vs NS, 2 tension with UW was also lower than the other solutions: UW, 35.8 ± 0.698 mmHg; NS, 48.5 ± 0.745 mmHg; MEC, 40.69 ± 0.749 mmHg; and M, 44.68 ± 0.697 mmHg. The calculated P value was UW vs NS, 0.0001; UW vs MEC, 0.0003; and UW vs M, 0.0001 using repeated-measures analysis of covariance. The W/D ratio was lower with UW as well: UW, 6.82 ± 0.19; NS, 8.01 ± 0.23; MEC, 7.28 ± 0.10; and M, 7.34 ± 0.17. The P value was",
keywords = "Preservation, Pulmonary, Solutions",
author = "Ghannam Al-Dossari and Shumway, {Sara J.}",
year = "1998",
month = "3",
doi = "10.1006/jsre.1998.5296",
language = "English (US)",
volume = "75",
pages = "187--191",
journal = "Journal of Surgical Research",
issn = "0022-4804",
publisher = "Academic Press Inc.",
number = "2",

}

TY - JOUR

T1 - Comparative study of solutions for pulmonary preservation using an isolated rabbit lung model

AU - Al-Dossari, Ghannam

AU - Shumway, Sara J.

PY - 1998/3

Y1 - 1998/3

N2 - At the University of Minnesota, University of Wisconsin (UW), modified Euro-Collins (MEC), and Marshall (M) solutions were compared as agents for pulmonary preservation in an isolated rabbit lung model. Normal saline (NS) was used as a control. The heart-lung blocks of donor rabbits were flushed with, and then preserved in, one of the solutions at 4°C. Five rabbits were studied in each group. After 8 h of cold ischemia, the left lung was ventilated and reperfused with fresh venous blood from donor rabbits for 30 min. Pulmonary function was assessed by serial measurements of oxygen (O2) and carbon dioxide (CO2) tensions in blood obtained from the left atrial appendage. The ratios of wet/dry (W/D) weight of the lungs were calculated to assess the extent of pulmonary edema. After 8 h of preservation followed by 30 min of reperfusion, O2 tension was significantly higher with UW (178.36 + 1.72 mmHg). The calculated P values were UW vs NS, 2 tension with UW was also lower than the other solutions: UW, 35.8 ± 0.698 mmHg; NS, 48.5 ± 0.745 mmHg; MEC, 40.69 ± 0.749 mmHg; and M, 44.68 ± 0.697 mmHg. The calculated P value was UW vs NS, 0.0001; UW vs MEC, 0.0003; and UW vs M, 0.0001 using repeated-measures analysis of covariance. The W/D ratio was lower with UW as well: UW, 6.82 ± 0.19; NS, 8.01 ± 0.23; MEC, 7.28 ± 0.10; and M, 7.34 ± 0.17. The P value was

AB - At the University of Minnesota, University of Wisconsin (UW), modified Euro-Collins (MEC), and Marshall (M) solutions were compared as agents for pulmonary preservation in an isolated rabbit lung model. Normal saline (NS) was used as a control. The heart-lung blocks of donor rabbits were flushed with, and then preserved in, one of the solutions at 4°C. Five rabbits were studied in each group. After 8 h of cold ischemia, the left lung was ventilated and reperfused with fresh venous blood from donor rabbits for 30 min. Pulmonary function was assessed by serial measurements of oxygen (O2) and carbon dioxide (CO2) tensions in blood obtained from the left atrial appendage. The ratios of wet/dry (W/D) weight of the lungs were calculated to assess the extent of pulmonary edema. After 8 h of preservation followed by 30 min of reperfusion, O2 tension was significantly higher with UW (178.36 + 1.72 mmHg). The calculated P values were UW vs NS, 2 tension with UW was also lower than the other solutions: UW, 35.8 ± 0.698 mmHg; NS, 48.5 ± 0.745 mmHg; MEC, 40.69 ± 0.749 mmHg; and M, 44.68 ± 0.697 mmHg. The calculated P value was UW vs NS, 0.0001; UW vs MEC, 0.0003; and UW vs M, 0.0001 using repeated-measures analysis of covariance. The W/D ratio was lower with UW as well: UW, 6.82 ± 0.19; NS, 8.01 ± 0.23; MEC, 7.28 ± 0.10; and M, 7.34 ± 0.17. The P value was

KW - Preservation

KW - Pulmonary

KW - Solutions

UR - http://www.scopus.com/inward/record.url?scp=0031777108&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0031777108&partnerID=8YFLogxK

U2 - 10.1006/jsre.1998.5296

DO - 10.1006/jsre.1998.5296

M3 - Article

C2 - 9655094

AN - SCOPUS:0031777108

VL - 75

SP - 187

EP - 191

JO - Journal of Surgical Research

JF - Journal of Surgical Research

SN - 0022-4804

IS - 2

ER -