Abstract
The domestic ferret is a uniformly lethal model of infection for 3 species of Ebolavirus known to be pathogenic in humans. Reagents to systematically analyze the ferret host response to infection are lacking; however, the recent publication of a draft ferret genome has opened the potential for transcriptional analysis of ferret models of disease. In this work, we present comparative analysis of longitudinally sampled blood taken from ferrets and nonhuman primates infected with lethal doses of the Makona variant of Zaire ebolavirus. Strong induction of proinflammatory and prothrombotic signaling programs were present in both ferrets and nonhuman primates, and both transcriptomes were similar to previously published datasets of fatal cases of human Ebola virus infection.
| Original language | English (US) |
|---|---|
| Pages (from-to) | S486-S495 |
| Journal | The Journal of infectious diseases |
| Volume | 218 |
| Issue number | 5 |
| DOIs | |
| State | Published - Nov 22 2018 |
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ASJC Scopus subject areas
- Immunology and Allergy
- Infectious Diseases
Cite this
Comparative Transcriptomics in Ebola Makona-Infected Ferrets, Nonhuman Primates, and Humans. / Cross, Robert; Speranza, Emily; Borisevich, Viktoriya; Widen, Steven; Wood, Thomas; Shim, Rebecca S.; Adams, Ricky D.; Gerhardt, Dawn M.; Bennett, Richard S.; Honko, Anna N.; Johnson, Joshua C.; Hensley, Lisa E.; Geisbert, Thomas; Connor, John H.
In: The Journal of infectious diseases, Vol. 218, No. 5, 22.11.2018, p. S486-S495.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Comparative Transcriptomics in Ebola Makona-Infected Ferrets, Nonhuman Primates, and Humans
AU - Cross, Robert
AU - Speranza, Emily
AU - Borisevich, Viktoriya
AU - Widen, Steven
AU - Wood, Thomas
AU - Shim, Rebecca S.
AU - Adams, Ricky D.
AU - Gerhardt, Dawn M.
AU - Bennett, Richard S.
AU - Honko, Anna N.
AU - Johnson, Joshua C.
AU - Hensley, Lisa E.
AU - Geisbert, Thomas
AU - Connor, John H.
PY - 2018/11/22
Y1 - 2018/11/22
N2 - The domestic ferret is a uniformly lethal model of infection for 3 species of Ebolavirus known to be pathogenic in humans. Reagents to systematically analyze the ferret host response to infection are lacking; however, the recent publication of a draft ferret genome has opened the potential for transcriptional analysis of ferret models of disease. In this work, we present comparative analysis of longitudinally sampled blood taken from ferrets and nonhuman primates infected with lethal doses of the Makona variant of Zaire ebolavirus. Strong induction of proinflammatory and prothrombotic signaling programs were present in both ferrets and nonhuman primates, and both transcriptomes were similar to previously published datasets of fatal cases of human Ebola virus infection.
AB - The domestic ferret is a uniformly lethal model of infection for 3 species of Ebolavirus known to be pathogenic in humans. Reagents to systematically analyze the ferret host response to infection are lacking; however, the recent publication of a draft ferret genome has opened the potential for transcriptional analysis of ferret models of disease. In this work, we present comparative analysis of longitudinally sampled blood taken from ferrets and nonhuman primates infected with lethal doses of the Makona variant of Zaire ebolavirus. Strong induction of proinflammatory and prothrombotic signaling programs were present in both ferrets and nonhuman primates, and both transcriptomes were similar to previously published datasets of fatal cases of human Ebola virus infection.
UR - http://www.scopus.com/inward/record.url?scp=85055633876&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=85055633876&partnerID=8YFLogxK
U2 - 10.1093/infdis/jiy455
DO - 10.1093/infdis/jiy455
M3 - Article
C2 - 30476250
AN - SCOPUS:85055633876
VL - 218
SP - S486-S495
JO - Journal of Infectious Diseases
JF - Journal of Infectious Diseases
SN - 0022-1899
IS - 5
ER -