@article{c8deac214a7343f79165e089949663a7,
title = "Comparative Transcriptomics in Ebola Makona-Infected Ferrets, Nonhuman Primates, and Humans",
abstract = "The domestic ferret is a uniformly lethal model of infection for 3 species of Ebolavirus known to be pathogenic in humans. Reagents to systematically analyze the ferret host response to infection are lacking; however, the recent publication of a draft ferret genome has opened the potential for transcriptional analysis of ferret models of disease. In this work, we present comparative analysis of longitudinally sampled blood taken from ferrets and nonhuman primates infected with lethal doses of the Makona variant of Zaire ebolavirus. Strong induction of proinflammatory and prothrombotic signaling programs were present in both ferrets and nonhuman primates, and both transcriptomes were similar to previously published datasets of fatal cases of human Ebola virus infection.",
keywords = "Ebola virus, animal model, ferret, transcriptomics, vaccines.",
author = "Cross, {Robert W.} and Emily Speranza and Viktoriya Borisevich and Widen, {Steven G.} and Wood, {Thomas G.} and Shim, {Rebecca S.} and Adams, {Ricky D.} and Gerhardt, {Dawn M.} and Bennett, {Richard S.} and Honko, {Anna N.} and Johnson, {Joshua C.} and Hensley, {Lisa E.} and Geisbert, {Thomas W.} and Connor, {John H.}",
note = "Funding Information: Acknowledgments. We thank Krystle Agans, Daniel Deer, Karla Fenton, Joan Geisbert, and Chad Mire for expert assistance with the biosafety level 4 animal studies; Dr. Kimberly Schuenke for administrative assistance; and Jessica Graber and the University of Texas Medical Branch Animal Resource Center for veterinary technical and husbandry support. We also thank Laura Bollinger (Integrated Research Facility) for critically editing the manuscript. Financial support. This work was supported by the Department of Microbiology and Immunology, University of Texas Medical Branch at Galveston, Galveston, TX (to T. W. G.). E. S. is supported by a National Science Foundation Graduate Research Fellowship under grant no. DGE-1247312. This work was also funded by National Institute of Allergy and Infectious Disease grant RO1AI1096159 (to J. H. C.). This work was partially funded by the Division of Intramural Research of the National Institute of Allergy and Infectious Diseases (NIAID), Division of Clinical Research, Integrated Research Facility and Battelle Memorial Institute{\textquoteright}s prime contract with NIAID (contract no. HHSN272200700016I). The nonhuman primates were provided by the Division of Microbiology and Infectious Diseases at NIAID.",
year = "2018",
month = nov,
day = "22",
doi = "10.1093/infdis/jiy455",
language = "English (US)",
volume = "218",
pages = "S486--S495",
journal = "Journal of Infectious Diseases",
issn = "0022-1899",
publisher = "Oxford University Press",
}