Comparative Transcriptomics in Ebola Makona-Infected Ferrets, Nonhuman Primates, and Humans

Robert W. Cross; Emily Speranza; Viktoriya Borisevich; Steven G. Widen; Thomas G. Wood; Rebecca S. Shim; Ricky D. Adams; Dawn M. Gerhardt; Richard S. Bennett; Anna N. Honko; Joshua C. Johnson; Lisa E. Hensley; Thomas W. Geisbert; John H. Connor

doi:10.1093/infdis/jiy455

Comparative Transcriptomics in Ebola Makona-Infected Ferrets, Nonhuman Primates, and Humans

Robert W. Cross, Emily Speranza, Viktoriya Borisevich, Steven G. Widen, Thomas G. Wood, Rebecca S. Shim, Ricky D. Adams, Dawn M. Gerhardt, Richard S. Bennett, Anna N. Honko, Joshua C. Johnson, Lisa E. Hensley, Thomas W. Geisbert, John H. Connor

Research output: Contribution to journal › Article › peer-review

24 Scopus citations

Abstract

The domestic ferret is a uniformly lethal model of infection for 3 species of Ebolavirus known to be pathogenic in humans. Reagents to systematically analyze the ferret host response to infection are lacking; however, the recent publication of a draft ferret genome has opened the potential for transcriptional analysis of ferret models of disease. In this work, we present comparative analysis of longitudinally sampled blood taken from ferrets and nonhuman primates infected with lethal doses of the Makona variant of Zaire ebolavirus. Strong induction of proinflammatory and prothrombotic signaling programs were present in both ferrets and nonhuman primates, and both transcriptomes were similar to previously published datasets of fatal cases of human Ebola virus infection.

Original language	English (US)
Pages (from-to)	S486-S495
Journal	Journal of Infectious Diseases
Volume	218
DOIs	https://doi.org/10.1093/infdis/jiy455
State	Published - Nov 22 2018

Keywords

Ebola virus
animal model
ferret
transcriptomics
vaccines.

ASJC Scopus subject areas

General Medicine

Access to Document

10.1093/infdis/jiy455

Cite this

Cross, R. W., Speranza, E., Borisevich, V., Widen, S. G., Wood, T. G., Shim, R. S., Adams, R. D., Gerhardt, D. M., Bennett, R. S., Honko, A. N., Johnson, J. C., Hensley, L. E., Geisbert, T. W., & Connor, J. H. (2018). Comparative Transcriptomics in Ebola Makona-Infected Ferrets, Nonhuman Primates, and Humans. Journal of Infectious Diseases, 218, S486-S495. https://doi.org/10.1093/infdis/jiy455

@article{c8deac214a7343f79165e089949663a7,

title = "Comparative Transcriptomics in Ebola Makona-Infected Ferrets, Nonhuman Primates, and Humans",

abstract = "The domestic ferret is a uniformly lethal model of infection for 3 species of Ebolavirus known to be pathogenic in humans. Reagents to systematically analyze the ferret host response to infection are lacking; however, the recent publication of a draft ferret genome has opened the potential for transcriptional analysis of ferret models of disease. In this work, we present comparative analysis of longitudinally sampled blood taken from ferrets and nonhuman primates infected with lethal doses of the Makona variant of Zaire ebolavirus. Strong induction of proinflammatory and prothrombotic signaling programs were present in both ferrets and nonhuman primates, and both transcriptomes were similar to previously published datasets of fatal cases of human Ebola virus infection.",

keywords = "Ebola virus, animal model, ferret, transcriptomics, vaccines.",

author = "Cross, {Robert W.} and Emily Speranza and Viktoriya Borisevich and Widen, {Steven G.} and Wood, {Thomas G.} and Shim, {Rebecca S.} and Adams, {Ricky D.} and Gerhardt, {Dawn M.} and Bennett, {Richard S.} and Honko, {Anna N.} and Johnson, {Joshua C.} and Hensley, {Lisa E.} and Geisbert, {Thomas W.} and Connor, {John H.}",

note = "Publisher Copyright: {\textcopyright} 2018. This article contains public sector information licensed under the Open Government Licence v3.0.",

year = "2018",

month = nov,

day = "22",

doi = "10.1093/infdis/jiy455",

language = "English (US)",

volume = "218",

pages = "S486--S495",

journal = "Journal of Infectious Diseases",

issn = "0022-1899",

publisher = "Oxford University Press",

}

TY - JOUR

T1 - Comparative Transcriptomics in Ebola Makona-Infected Ferrets, Nonhuman Primates, and Humans

AU - Cross, Robert W.

AU - Speranza, Emily

AU - Borisevich, Viktoriya

AU - Widen, Steven G.

AU - Wood, Thomas G.

AU - Shim, Rebecca S.

AU - Adams, Ricky D.

AU - Gerhardt, Dawn M.

AU - Bennett, Richard S.

AU - Honko, Anna N.

AU - Johnson, Joshua C.

AU - Hensley, Lisa E.

AU - Geisbert, Thomas W.

AU - Connor, John H.

PY - 2018/11/22

Y1 - 2018/11/22

N2 - The domestic ferret is a uniformly lethal model of infection for 3 species of Ebolavirus known to be pathogenic in humans. Reagents to systematically analyze the ferret host response to infection are lacking; however, the recent publication of a draft ferret genome has opened the potential for transcriptional analysis of ferret models of disease. In this work, we present comparative analysis of longitudinally sampled blood taken from ferrets and nonhuman primates infected with lethal doses of the Makona variant of Zaire ebolavirus. Strong induction of proinflammatory and prothrombotic signaling programs were present in both ferrets and nonhuman primates, and both transcriptomes were similar to previously published datasets of fatal cases of human Ebola virus infection.

AB - The domestic ferret is a uniformly lethal model of infection for 3 species of Ebolavirus known to be pathogenic in humans. Reagents to systematically analyze the ferret host response to infection are lacking; however, the recent publication of a draft ferret genome has opened the potential for transcriptional analysis of ferret models of disease. In this work, we present comparative analysis of longitudinally sampled blood taken from ferrets and nonhuman primates infected with lethal doses of the Makona variant of Zaire ebolavirus. Strong induction of proinflammatory and prothrombotic signaling programs were present in both ferrets and nonhuman primates, and both transcriptomes were similar to previously published datasets of fatal cases of human Ebola virus infection.

KW - Ebola virus

KW - animal model

KW - ferret

KW - transcriptomics

KW - vaccines.

UR - http://www.scopus.com/inward/record.url?scp=85055633876&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=85055633876&partnerID=8YFLogxK

U2 - 10.1093/infdis/jiy455

DO - 10.1093/infdis/jiy455

M3 - Article

C2 - 30476250

AN - SCOPUS:85055633876

SN - 0022-1899

VL - 218

SP - S486-S495

JO - Journal of Infectious Diseases

JF - Journal of Infectious Diseases

ER -

Comparative Transcriptomics in Ebola Makona-Infected Ferrets, Nonhuman Primates, and Humans

Abstract

Keywords

ASJC Scopus subject areas

Access to Document

Other files and links

Fingerprint

Cite this