Comparison between the responses mediated by endogenous and expressed ATP-and GABA-receptors in Xenopus laevis oocytes

Qing Yang, Yi Xu, Li Zhi Wang

Research output: Contribution to journalArticle

Abstract

Objective: To study the comparison between the responses mediated by endogenous and expressed ATP-and GABA-receptors in Xenopus laevis oocytes. Methods: The two-electrode voltage-clamp technique was used. Results: (1) Zn2+ (10-5 mol/L) could potentiate the current responses mediated by expressed P2X2 and P2X4 receptors in oocytes; (2) GABA-activated current in the oocytes which were injected mRNA of chicken brain could be blocked by bicuculline (10-5 mol/L), a selective GABAA receptor antagonist; (3) GABA-activated current mediated by ρ1 subunit of human retina could not be blocked by bicuculline (10-5 mol/L), but it could be abolished by picrotoxin (10-5 mol/L); (4) there were three types ATP-activated current in the follicular oocytes, while there was only one type GABA-activated current; (5) there were no responses mediated by ATP or GABA receptors in the defollicular oocytes. Conclusion: There exist endogenous GABAA and GABAc receptors and ATP receptors in the follicular oocytes. The current responses mediated by exogenous GABA receptors and P2X2 and P2X4 receptors are different from that mediated by endogenous GABA receptors and ATP receptors.

Original languageEnglish (US)
JournalMedical Journal of Wuhan University
Volume23
Issue number3
StatePublished - Jul 2002
Externally publishedYes

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Purinergic P2 Receptors
GABA Receptors
Xenopus laevis
Oocytes
Purinergic P2X4 Receptors
Purinergic P2X2 Receptors
gamma-Aminobutyric Acid
Bicuculline
GABA-A Receptor Antagonists
Picrotoxin
Patch-Clamp Techniques
GABA-A Receptors
Retina
Chickens
Electrodes
Adenosine Triphosphate
Messenger RNA
Brain

Keywords

  • Endogenous ATP receptor
  • Endogenous GABA receptor
  • Xenopus laevis oocyte

ASJC Scopus subject areas

  • Medicine(all)

Cite this

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title = "Comparison between the responses mediated by endogenous and expressed ATP-and GABA-receptors in Xenopus laevis oocytes",
abstract = "Objective: To study the comparison between the responses mediated by endogenous and expressed ATP-and GABA-receptors in Xenopus laevis oocytes. Methods: The two-electrode voltage-clamp technique was used. Results: (1) Zn2+ (10-5 mol/L) could potentiate the current responses mediated by expressed P2X2 and P2X4 receptors in oocytes; (2) GABA-activated current in the oocytes which were injected mRNA of chicken brain could be blocked by bicuculline (10-5 mol/L), a selective GABAA receptor antagonist; (3) GABA-activated current mediated by ρ1 subunit of human retina could not be blocked by bicuculline (10-5 mol/L), but it could be abolished by picrotoxin (10-5 mol/L); (4) there were three types ATP-activated current in the follicular oocytes, while there was only one type GABA-activated current; (5) there were no responses mediated by ATP or GABA receptors in the defollicular oocytes. Conclusion: There exist endogenous GABAA and GABAc receptors and ATP receptors in the follicular oocytes. The current responses mediated by exogenous GABA receptors and P2X2 and P2X4 receptors are different from that mediated by endogenous GABA receptors and ATP receptors.",
keywords = "Endogenous ATP receptor, Endogenous GABA receptor, Xenopus laevis oocyte",
author = "Qing Yang and Yi Xu and Wang, {Li Zhi}",
year = "2002",
month = "7",
language = "English (US)",
volume = "23",
journal = "Medical Journal of Wuhan University",
issn = "1671-8852",
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TY - JOUR

T1 - Comparison between the responses mediated by endogenous and expressed ATP-and GABA-receptors in Xenopus laevis oocytes

AU - Yang, Qing

AU - Xu, Yi

AU - Wang, Li Zhi

PY - 2002/7

Y1 - 2002/7

N2 - Objective: To study the comparison between the responses mediated by endogenous and expressed ATP-and GABA-receptors in Xenopus laevis oocytes. Methods: The two-electrode voltage-clamp technique was used. Results: (1) Zn2+ (10-5 mol/L) could potentiate the current responses mediated by expressed P2X2 and P2X4 receptors in oocytes; (2) GABA-activated current in the oocytes which were injected mRNA of chicken brain could be blocked by bicuculline (10-5 mol/L), a selective GABAA receptor antagonist; (3) GABA-activated current mediated by ρ1 subunit of human retina could not be blocked by bicuculline (10-5 mol/L), but it could be abolished by picrotoxin (10-5 mol/L); (4) there were three types ATP-activated current in the follicular oocytes, while there was only one type GABA-activated current; (5) there were no responses mediated by ATP or GABA receptors in the defollicular oocytes. Conclusion: There exist endogenous GABAA and GABAc receptors and ATP receptors in the follicular oocytes. The current responses mediated by exogenous GABA receptors and P2X2 and P2X4 receptors are different from that mediated by endogenous GABA receptors and ATP receptors.

AB - Objective: To study the comparison between the responses mediated by endogenous and expressed ATP-and GABA-receptors in Xenopus laevis oocytes. Methods: The two-electrode voltage-clamp technique was used. Results: (1) Zn2+ (10-5 mol/L) could potentiate the current responses mediated by expressed P2X2 and P2X4 receptors in oocytes; (2) GABA-activated current in the oocytes which were injected mRNA of chicken brain could be blocked by bicuculline (10-5 mol/L), a selective GABAA receptor antagonist; (3) GABA-activated current mediated by ρ1 subunit of human retina could not be blocked by bicuculline (10-5 mol/L), but it could be abolished by picrotoxin (10-5 mol/L); (4) there were three types ATP-activated current in the follicular oocytes, while there was only one type GABA-activated current; (5) there were no responses mediated by ATP or GABA receptors in the defollicular oocytes. Conclusion: There exist endogenous GABAA and GABAc receptors and ATP receptors in the follicular oocytes. The current responses mediated by exogenous GABA receptors and P2X2 and P2X4 receptors are different from that mediated by endogenous GABA receptors and ATP receptors.

KW - Endogenous ATP receptor

KW - Endogenous GABA receptor

KW - Xenopus laevis oocyte

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