Comparison of airway and blood eosinophil function after in vivo antigen challenge

Julie B. Sedgwick, William Calhoun, Rose F. Vrtis, Mary Ellen Bates, Pamela K. Mcallister, William W. Busse

Research output: Contribution to journalArticle

178 Citations (Scopus)

Abstract

Eosinophils (EOS) are important effector cells in allergic diseases and asthma. However, functional characteristics of the EOS have been derived primarily from studies of blood cells, and it is unlikely that such assessments reflect events occurring in tissues or airways. To establish more precisely the function of airway EOS, segmental Ag challenge was used to elicit and isolate large numbers of these cells. Airway, as well as blood, EOS were isolated from allergic patients 48 h after segmental Ag challenge. Both blood and bronchoalveolar lavage (BAL) EOS were fractionated over Percoll density gradients; by using this protocol, three density-distinct populations of pure (>90%) EOS were obtained from BAL fluid (1.100, 1.095, and 1.090 g/ml) and one from blood (1.100 g/ml). The functions of these various populations were compared by measuring superoxide generation, adherence to collagen and endothelial cell monolayers, cell surface receptors, and in vitro survival. BAL EOS of all three densities had greater superoxide generation and adherence with FMLP activation than did corresponding blood EOS. In contrast, blood and airway EOS responded similarly to PMA. BAL EOS also had increased expression of CD11b/CD18 and HLA-DR. The intracellular calcium concentration ([Ca2+]i) was measured with the fluorescent marker indo-1/acetoxymethyl ester. FMLP caused a greater and more sustained increase in [Ca2+]i with BAL than blood EOS. EGTA blocked the sustained component of the [Ca2+]i response to FMLP. Our findings indicate that BAL EOS have an enhanced [Ca2+]i response to activation that may contribute to their functional up-regulation.

Original languageEnglish (US)
Pages (from-to)3710-3718
Number of pages9
JournalJournal of Immunology
Volume149
Issue number11
StatePublished - 1992
Externally publishedYes

Fingerprint

Eosinophils
Antigens
Bronchoalveolar Lavage
Superoxides
Egtazic Acid
Bronchoalveolar Lavage Fluid
Cell Surface Receptors
HLA-DR Antigens
Population Density
Blood Cells
Esters
Up-Regulation
Collagen
Asthma
Endothelial Cells
Cell Count
Calcium
Survival

ASJC Scopus subject areas

  • Immunology

Cite this

Sedgwick, J. B., Calhoun, W., Vrtis, R. F., Bates, M. E., Mcallister, P. K., & Busse, W. W. (1992). Comparison of airway and blood eosinophil function after in vivo antigen challenge. Journal of Immunology, 149(11), 3710-3718.

Comparison of airway and blood eosinophil function after in vivo antigen challenge. / Sedgwick, Julie B.; Calhoun, William; Vrtis, Rose F.; Bates, Mary Ellen; Mcallister, Pamela K.; Busse, William W.

In: Journal of Immunology, Vol. 149, No. 11, 1992, p. 3710-3718.

Research output: Contribution to journalArticle

Sedgwick, JB, Calhoun, W, Vrtis, RF, Bates, ME, Mcallister, PK & Busse, WW 1992, 'Comparison of airway and blood eosinophil function after in vivo antigen challenge', Journal of Immunology, vol. 149, no. 11, pp. 3710-3718.
Sedgwick JB, Calhoun W, Vrtis RF, Bates ME, Mcallister PK, Busse WW. Comparison of airway and blood eosinophil function after in vivo antigen challenge. Journal of Immunology. 1992;149(11):3710-3718.
Sedgwick, Julie B. ; Calhoun, William ; Vrtis, Rose F. ; Bates, Mary Ellen ; Mcallister, Pamela K. ; Busse, William W. / Comparison of airway and blood eosinophil function after in vivo antigen challenge. In: Journal of Immunology. 1992 ; Vol. 149, No. 11. pp. 3710-3718.
@article{e8403aca7f914ee094bc311f95267bca,
title = "Comparison of airway and blood eosinophil function after in vivo antigen challenge",
abstract = "Eosinophils (EOS) are important effector cells in allergic diseases and asthma. However, functional characteristics of the EOS have been derived primarily from studies of blood cells, and it is unlikely that such assessments reflect events occurring in tissues or airways. To establish more precisely the function of airway EOS, segmental Ag challenge was used to elicit and isolate large numbers of these cells. Airway, as well as blood, EOS were isolated from allergic patients 48 h after segmental Ag challenge. Both blood and bronchoalveolar lavage (BAL) EOS were fractionated over Percoll density gradients; by using this protocol, three density-distinct populations of pure (>90{\%}) EOS were obtained from BAL fluid (1.100, 1.095, and 1.090 g/ml) and one from blood (1.100 g/ml). The functions of these various populations were compared by measuring superoxide generation, adherence to collagen and endothelial cell monolayers, cell surface receptors, and in vitro survival. BAL EOS of all three densities had greater superoxide generation and adherence with FMLP activation than did corresponding blood EOS. In contrast, blood and airway EOS responded similarly to PMA. BAL EOS also had increased expression of CD11b/CD18 and HLA-DR. The intracellular calcium concentration ([Ca2+]i) was measured with the fluorescent marker indo-1/acetoxymethyl ester. FMLP caused a greater and more sustained increase in [Ca2+]i with BAL than blood EOS. EGTA blocked the sustained component of the [Ca2+]i response to FMLP. Our findings indicate that BAL EOS have an enhanced [Ca2+]i response to activation that may contribute to their functional up-regulation.",
author = "Sedgwick, {Julie B.} and William Calhoun and Vrtis, {Rose F.} and Bates, {Mary Ellen} and Mcallister, {Pamela K.} and Busse, {William W.}",
year = "1992",
language = "English (US)",
volume = "149",
pages = "3710--3718",
journal = "Journal of Immunology",
issn = "0022-1767",
publisher = "American Association of Immunologists",
number = "11",

}

TY - JOUR

T1 - Comparison of airway and blood eosinophil function after in vivo antigen challenge

AU - Sedgwick, Julie B.

AU - Calhoun, William

AU - Vrtis, Rose F.

AU - Bates, Mary Ellen

AU - Mcallister, Pamela K.

AU - Busse, William W.

PY - 1992

Y1 - 1992

N2 - Eosinophils (EOS) are important effector cells in allergic diseases and asthma. However, functional characteristics of the EOS have been derived primarily from studies of blood cells, and it is unlikely that such assessments reflect events occurring in tissues or airways. To establish more precisely the function of airway EOS, segmental Ag challenge was used to elicit and isolate large numbers of these cells. Airway, as well as blood, EOS were isolated from allergic patients 48 h after segmental Ag challenge. Both blood and bronchoalveolar lavage (BAL) EOS were fractionated over Percoll density gradients; by using this protocol, three density-distinct populations of pure (>90%) EOS were obtained from BAL fluid (1.100, 1.095, and 1.090 g/ml) and one from blood (1.100 g/ml). The functions of these various populations were compared by measuring superoxide generation, adherence to collagen and endothelial cell monolayers, cell surface receptors, and in vitro survival. BAL EOS of all three densities had greater superoxide generation and adherence with FMLP activation than did corresponding blood EOS. In contrast, blood and airway EOS responded similarly to PMA. BAL EOS also had increased expression of CD11b/CD18 and HLA-DR. The intracellular calcium concentration ([Ca2+]i) was measured with the fluorescent marker indo-1/acetoxymethyl ester. FMLP caused a greater and more sustained increase in [Ca2+]i with BAL than blood EOS. EGTA blocked the sustained component of the [Ca2+]i response to FMLP. Our findings indicate that BAL EOS have an enhanced [Ca2+]i response to activation that may contribute to their functional up-regulation.

AB - Eosinophils (EOS) are important effector cells in allergic diseases and asthma. However, functional characteristics of the EOS have been derived primarily from studies of blood cells, and it is unlikely that such assessments reflect events occurring in tissues or airways. To establish more precisely the function of airway EOS, segmental Ag challenge was used to elicit and isolate large numbers of these cells. Airway, as well as blood, EOS were isolated from allergic patients 48 h after segmental Ag challenge. Both blood and bronchoalveolar lavage (BAL) EOS were fractionated over Percoll density gradients; by using this protocol, three density-distinct populations of pure (>90%) EOS were obtained from BAL fluid (1.100, 1.095, and 1.090 g/ml) and one from blood (1.100 g/ml). The functions of these various populations were compared by measuring superoxide generation, adherence to collagen and endothelial cell monolayers, cell surface receptors, and in vitro survival. BAL EOS of all three densities had greater superoxide generation and adherence with FMLP activation than did corresponding blood EOS. In contrast, blood and airway EOS responded similarly to PMA. BAL EOS also had increased expression of CD11b/CD18 and HLA-DR. The intracellular calcium concentration ([Ca2+]i) was measured with the fluorescent marker indo-1/acetoxymethyl ester. FMLP caused a greater and more sustained increase in [Ca2+]i with BAL than blood EOS. EGTA blocked the sustained component of the [Ca2+]i response to FMLP. Our findings indicate that BAL EOS have an enhanced [Ca2+]i response to activation that may contribute to their functional up-regulation.

UR - http://www.scopus.com/inward/record.url?scp=0026485410&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0026485410&partnerID=8YFLogxK

M3 - Article

C2 - 1358975

AN - SCOPUS:0026485410

VL - 149

SP - 3710

EP - 3718

JO - Journal of Immunology

JF - Journal of Immunology

SN - 0022-1767

IS - 11

ER -