Abstract
The Mycobacterium tuberculosis ESX-1 secreted protein regulator (EspR, Rv3849) is the key protein that delivers bacterial proteins into the host cell during mycobacterial infection. EspR binds directly to the espACD operon and is involved in transcriptional activation. In the current study, M. tuberculosis EspR has been crystallized and its X-ray structure has been determined at 3.3 Å resolution in a P3221 crystal form. EspR forms a physiological dimer in the crystal. Each EspR monomer contains an N-terminal helix-turn-helix DNA-binding domain and a C-terminal dimerization domain. The EspR structure in the P3221 crystal form was compared with previously determined EspR structures in P32, P21 and P212121 crystal forms. Structural comparison analysis indicated that the N-terminal helix-turn-helix domain of EspR acquires a rigid structure in the four crystal forms. However, significant structural differences were observed in the C-terminal domain of EspR in the P21 crystal form when compared with the P3221 and P32 crystal forms. The interaction, stabilization energy and buried surface area analysis of EspR in the four different crystal forms have provided information about the physiological dimer interface of EspR.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 433-437 |
| Number of pages | 5 |
| Journal | Acta Crystallographica Section F:Structural Biology Communications |
| Volume | 70 |
| Issue number | 4 |
| DOIs | |
| State | Published - 2014 |
| Externally published | Yes |
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Keywords
- M. tuberculosis EspR
ASJC Scopus subject areas
- Biochemistry
- Biophysics
- Genetics
- Structural Biology
- Condensed Matter Physics
- Medicine(all)
Cite this
Comparison of four different crystal forms of the Mycobacterium tuberculosis ESX-1 secreted protein regulator EspR. / Gangwar, Shanti; Meena, Sita R.; Saxena, Ajay K.
In: Acta Crystallographica Section F:Structural Biology Communications, Vol. 70, No. 4, 2014, p. 433-437.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Comparison of four different crystal forms of the Mycobacterium tuberculosis ESX-1 secreted protein regulator EspR
AU - Gangwar, Shanti
AU - Meena, Sita R.
AU - Saxena, Ajay K.
PY - 2014
Y1 - 2014
N2 - The Mycobacterium tuberculosis ESX-1 secreted protein regulator (EspR, Rv3849) is the key protein that delivers bacterial proteins into the host cell during mycobacterial infection. EspR binds directly to the espACD operon and is involved in transcriptional activation. In the current study, M. tuberculosis EspR has been crystallized and its X-ray structure has been determined at 3.3 Å resolution in a P3221 crystal form. EspR forms a physiological dimer in the crystal. Each EspR monomer contains an N-terminal helix-turn-helix DNA-binding domain and a C-terminal dimerization domain. The EspR structure in the P3221 crystal form was compared with previously determined EspR structures in P32, P21 and P212121 crystal forms. Structural comparison analysis indicated that the N-terminal helix-turn-helix domain of EspR acquires a rigid structure in the four crystal forms. However, significant structural differences were observed in the C-terminal domain of EspR in the P21 crystal form when compared with the P3221 and P32 crystal forms. The interaction, stabilization energy and buried surface area analysis of EspR in the four different crystal forms have provided information about the physiological dimer interface of EspR.
AB - The Mycobacterium tuberculosis ESX-1 secreted protein regulator (EspR, Rv3849) is the key protein that delivers bacterial proteins into the host cell during mycobacterial infection. EspR binds directly to the espACD operon and is involved in transcriptional activation. In the current study, M. tuberculosis EspR has been crystallized and its X-ray structure has been determined at 3.3 Å resolution in a P3221 crystal form. EspR forms a physiological dimer in the crystal. Each EspR monomer contains an N-terminal helix-turn-helix DNA-binding domain and a C-terminal dimerization domain. The EspR structure in the P3221 crystal form was compared with previously determined EspR structures in P32, P21 and P212121 crystal forms. Structural comparison analysis indicated that the N-terminal helix-turn-helix domain of EspR acquires a rigid structure in the four crystal forms. However, significant structural differences were observed in the C-terminal domain of EspR in the P21 crystal form when compared with the P3221 and P32 crystal forms. The interaction, stabilization energy and buried surface area analysis of EspR in the four different crystal forms have provided information about the physiological dimer interface of EspR.
KW - M. tuberculosis EspR
UR - http://www.scopus.com/inward/record.url?scp=84905436015&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84905436015&partnerID=8YFLogxK
U2 - 10.1107/S2053230X14004166
DO - 10.1107/S2053230X14004166
M3 - Article
VL - 70
SP - 433
EP - 437
JO - Acta Crystallographica Section F:Structural Biology Communications
JF - Acta Crystallographica Section F:Structural Biology Communications
SN - 1744-3091
IS - 4
ER -