Comparison of GH, IGF-I, and testosterone with mRNA of receptors and myostatin in skeletal muscle in older men

Taylor J. Marcell, S. Mitchell Harman, Randall Urban, Daniel D. Metz, Buel D. Rodgers, Marc R. Blackman

Research output: Contribution to journalArticle

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Abstract

Growth hormone (GH), insulin-like growth factor I (IGF-I), and testosterone (T) are important mediators of muscle protein synthesis, and thus muscle mass, all of which decline with age. We hypothesized that circulating hormones would be related to the transcriptional levels of their respective receptors and that this expression would be negatively related to expression of the myostatin gene. We therefore determined content of mRNA transcripts (by RT-PCR) for GH receptor (GHR), IGF-I, androgen receptor (AR), and myostatin in skeletal muscle biopsy samples from 27 healthy men <65 yr of age. There were no significant relationships between age, lean body mass, or percent body fat and transcript levels of GHR, IGF-I, AR, or myostatin. Moreover, there were no significant correlations of serum GH, IGF-I, or T with their corresponding target mRNA levels (GHR, intramuscular IGF-I, or AR) in skeletal muscle. However, GHR was negatively correlated (r = -0.60, P = 0.001) with myostatin mRNA levels. The lack of apparent relationships of muscle transcripts with their respective ligands in healthy older adults suggests that age-related deficits in both GH and T may lead to an increase in myostatin expression and a disassociation in autocrine IGF-I effects on muscle protein synthesis, both of which could contribute to age-related sarcopenia.

Original languageEnglish (US)
JournalAmerican Journal of Physiology - Endocrinology and Metabolism
Volume281
Issue number6 44-6
StatePublished - 2001
Externally publishedYes

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Myostatin
Insulin-Like Growth Factor I
Growth Hormone
Muscle
Testosterone
Skeletal Muscle
IGF Type 1 Receptor
Androgen Receptors
Messenger RNA
Muscle Proteins
Sarcopenia
Somatotropin Receptors
Muscles
Biopsy
Adipose Tissue
Genes
Fats
Hormones
Ligands
Gene Expression

Keywords

  • Androgens
  • Elderly
  • Gene expression
  • Polymerase chain reaction
  • Protein metabolism
  • Reverse transcription

ASJC Scopus subject areas

  • Physiology
  • Endocrinology
  • Biochemistry
  • Physiology (medical)

Cite this

Comparison of GH, IGF-I, and testosterone with mRNA of receptors and myostatin in skeletal muscle in older men. / Marcell, Taylor J.; Mitchell Harman, S.; Urban, Randall; Metz, Daniel D.; Rodgers, Buel D.; Blackman, Marc R.

In: American Journal of Physiology - Endocrinology and Metabolism, Vol. 281, No. 6 44-6, 2001.

Research output: Contribution to journalArticle

Marcell, Taylor J. ; Mitchell Harman, S. ; Urban, Randall ; Metz, Daniel D. ; Rodgers, Buel D. ; Blackman, Marc R. / Comparison of GH, IGF-I, and testosterone with mRNA of receptors and myostatin in skeletal muscle in older men. In: American Journal of Physiology - Endocrinology and Metabolism. 2001 ; Vol. 281, No. 6 44-6.
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