Comparison of in vitro and in vivo systems for propagation of rift valley fever virus from clinical specimens

G. W. Anderson, J. F. Saluzzo, Thomas Ksiazek, J. F. Smith, W. Ennis, D. Thureen, C. J. Peters, J. P. Digoutte

Research output: Contribution to journalArticle

20 Citations (Scopus)

Abstract

Several cell cultures and animals were compared for their relative sensitivity as primary isolation systems for Rift Valley fever virus (RVFV) and to determine if virulence characteristics of the isolates were altered in these systems. Eleven human sera from known cases of Rift Valley fever (RVF) were obtained from the 1987 epidemic in Mauritania and served as the source of virus for these studies. Sera were inoculated directly into cell cultures (Vero, C6/36 and DBS-FRhL-2) and animals (ICR suckling mice, Lak:LVG(SYR) hamsters and WF rats) concurrently. The cell lines provided a quick method to propagate, quantitate and identify these specimens without prior adaption. The isolates were highly virulent for suckling mice and hamsters, but not for WF rats, even after cell culture passage, which indicated that the Mauritanian isolates more closely resembled those strains from sub-Saharan Africa than those from the 1977-78 Egyptian epidemic.

Original languageEnglish (US)
Pages (from-to)129-138
Number of pages10
JournalResearch in Virology
Volume140
Issue numberC
DOIs
StatePublished - 1989
Externally publishedYes

Fingerprint

Rift Valley fever virus
Inbred WF Rats
Cell Culture Techniques
Cricetinae
Suckling Animals
Mauritania
Rift Valley Fever
Inbred ICR Mouse
Africa South of the Sahara
Serum
Virulence
Viruses
Cell Line
In Vitro Techniques

Keywords

  • Phlebovirus, Bunyaviridae, Mauritania, In vitro and in vivo
  • RVF, Virulence, Cytopathology

ASJC Scopus subject areas

  • Immunology
  • Virology

Cite this

Comparison of in vitro and in vivo systems for propagation of rift valley fever virus from clinical specimens. / Anderson, G. W.; Saluzzo, J. F.; Ksiazek, Thomas; Smith, J. F.; Ennis, W.; Thureen, D.; Peters, C. J.; Digoutte, J. P.

In: Research in Virology, Vol. 140, No. C, 1989, p. 129-138.

Research output: Contribution to journalArticle

Anderson, GW, Saluzzo, JF, Ksiazek, T, Smith, JF, Ennis, W, Thureen, D, Peters, CJ & Digoutte, JP 1989, 'Comparison of in vitro and in vivo systems for propagation of rift valley fever virus from clinical specimens', Research in Virology, vol. 140, no. C, pp. 129-138. https://doi.org/10.1016/S0923-2516(89)80090-1
Anderson, G. W. ; Saluzzo, J. F. ; Ksiazek, Thomas ; Smith, J. F. ; Ennis, W. ; Thureen, D. ; Peters, C. J. ; Digoutte, J. P. / Comparison of in vitro and in vivo systems for propagation of rift valley fever virus from clinical specimens. In: Research in Virology. 1989 ; Vol. 140, No. C. pp. 129-138.
@article{61e60041fac64a35ae0fb79ba35418f9,
title = "Comparison of in vitro and in vivo systems for propagation of rift valley fever virus from clinical specimens",
abstract = "Several cell cultures and animals were compared for their relative sensitivity as primary isolation systems for Rift Valley fever virus (RVFV) and to determine if virulence characteristics of the isolates were altered in these systems. Eleven human sera from known cases of Rift Valley fever (RVF) were obtained from the 1987 epidemic in Mauritania and served as the source of virus for these studies. Sera were inoculated directly into cell cultures (Vero, C6/36 and DBS-FRhL-2) and animals (ICR suckling mice, Lak:LVG(SYR) hamsters and WF rats) concurrently. The cell lines provided a quick method to propagate, quantitate and identify these specimens without prior adaption. The isolates were highly virulent for suckling mice and hamsters, but not for WF rats, even after cell culture passage, which indicated that the Mauritanian isolates more closely resembled those strains from sub-Saharan Africa than those from the 1977-78 Egyptian epidemic.",
keywords = "Phlebovirus, Bunyaviridae, Mauritania, In vitro and in vivo, RVF, Virulence, Cytopathology",
author = "Anderson, {G. W.} and Saluzzo, {J. F.} and Thomas Ksiazek and Smith, {J. F.} and W. Ennis and D. Thureen and Peters, {C. J.} and Digoutte, {J. P.}",
year = "1989",
doi = "10.1016/S0923-2516(89)80090-1",
language = "English (US)",
volume = "140",
pages = "129--138",
journal = "Research in Virology",
issn = "0923-2516",
publisher = "Elsevier BV",
number = "C",

}

TY - JOUR

T1 - Comparison of in vitro and in vivo systems for propagation of rift valley fever virus from clinical specimens

AU - Anderson, G. W.

AU - Saluzzo, J. F.

AU - Ksiazek, Thomas

AU - Smith, J. F.

AU - Ennis, W.

AU - Thureen, D.

AU - Peters, C. J.

AU - Digoutte, J. P.

PY - 1989

Y1 - 1989

N2 - Several cell cultures and animals were compared for their relative sensitivity as primary isolation systems for Rift Valley fever virus (RVFV) and to determine if virulence characteristics of the isolates were altered in these systems. Eleven human sera from known cases of Rift Valley fever (RVF) were obtained from the 1987 epidemic in Mauritania and served as the source of virus for these studies. Sera were inoculated directly into cell cultures (Vero, C6/36 and DBS-FRhL-2) and animals (ICR suckling mice, Lak:LVG(SYR) hamsters and WF rats) concurrently. The cell lines provided a quick method to propagate, quantitate and identify these specimens without prior adaption. The isolates were highly virulent for suckling mice and hamsters, but not for WF rats, even after cell culture passage, which indicated that the Mauritanian isolates more closely resembled those strains from sub-Saharan Africa than those from the 1977-78 Egyptian epidemic.

AB - Several cell cultures and animals were compared for their relative sensitivity as primary isolation systems for Rift Valley fever virus (RVFV) and to determine if virulence characteristics of the isolates were altered in these systems. Eleven human sera from known cases of Rift Valley fever (RVF) were obtained from the 1987 epidemic in Mauritania and served as the source of virus for these studies. Sera were inoculated directly into cell cultures (Vero, C6/36 and DBS-FRhL-2) and animals (ICR suckling mice, Lak:LVG(SYR) hamsters and WF rats) concurrently. The cell lines provided a quick method to propagate, quantitate and identify these specimens without prior adaption. The isolates were highly virulent for suckling mice and hamsters, but not for WF rats, even after cell culture passage, which indicated that the Mauritanian isolates more closely resembled those strains from sub-Saharan Africa than those from the 1977-78 Egyptian epidemic.

KW - Phlebovirus, Bunyaviridae, Mauritania, In vitro and in vivo

KW - RVF, Virulence, Cytopathology

UR - http://www.scopus.com/inward/record.url?scp=0024594213&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0024594213&partnerID=8YFLogxK

U2 - 10.1016/S0923-2516(89)80090-1

DO - 10.1016/S0923-2516(89)80090-1

M3 - Article

VL - 140

SP - 129

EP - 138

JO - Research in Virology

JF - Research in Virology

SN - 0923-2516

IS - C

ER -