Comparison of in vitro and in vivo systems for propagation of rift valley fever virus from clinical specimens

G. W. Anderson; J. F. Saluzzo; T. G. Ksiazek; J. F. Smith; W. Ennis; D. Thureen; C. J. Peters; J. P. Digoutte

doi:10.1016/S0923-2516(89)80090-1

Comparison of in vitro and in vivo systems for propagation of rift valley fever virus from clinical specimens

G. W. Anderson, J. F. Saluzzo, T. G. Ksiazek, J. F. Smith, W. Ennis, D. Thureen, C. J. Peters, J. P. Digoutte

Research output: Contribution to journal › Article › peer-review

27 Scopus citations

Abstract

Several cell cultures and animals were compared for their relative sensitivity as primary isolation systems for Rift Valley fever virus (RVFV) and to determine if virulence characteristics of the isolates were altered in these systems. Eleven human sera from known cases of Rift Valley fever (RVF) were obtained from the 1987 epidemic in Mauritania and served as the source of virus for these studies. Sera were inoculated directly into cell cultures (Vero, C6/36 and DBS-FRhL-2) and animals (ICR suckling mice, Lak:LVG(SYR) hamsters and WF rats) concurrently. The cell lines provided a quick method to propagate, quantitate and identify these specimens without prior adaption. The isolates were highly virulent for suckling mice and hamsters, but not for WF rats, even after cell culture passage, which indicated that the Mauritanian isolates more closely resembled those strains from sub-Saharan Africa than those from the 1977-78 Egyptian epidemic.

Original language	English (US)
Pages (from-to)	129-138
Number of pages	10
Journal	Research in Virology
Volume	140
Issue number	C
DOIs	https://doi.org/10.1016/S0923-2516(89)80090-1
State	Published - 1989
Externally published	Yes

Keywords

Phlebovirus, Bunyaviridae, Mauritania, In vitro and in vivo
RVF, Virulence, Cytopathology

ASJC Scopus subject areas

Immunology
Virology

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10.1016/S0923-2516(89)80090-1

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title = "Comparison of in vitro and in vivo systems for propagation of rift valley fever virus from clinical specimens",

abstract = "Several cell cultures and animals were compared for their relative sensitivity as primary isolation systems for Rift Valley fever virus (RVFV) and to determine if virulence characteristics of the isolates were altered in these systems. Eleven human sera from known cases of Rift Valley fever (RVF) were obtained from the 1987 epidemic in Mauritania and served as the source of virus for these studies. Sera were inoculated directly into cell cultures (Vero, C6/36 and DBS-FRhL-2) and animals (ICR suckling mice, Lak:LVG(SYR) hamsters and WF rats) concurrently. The cell lines provided a quick method to propagate, quantitate and identify these specimens without prior adaption. The isolates were highly virulent for suckling mice and hamsters, but not for WF rats, even after cell culture passage, which indicated that the Mauritanian isolates more closely resembled those strains from sub-Saharan Africa than those from the 1977-78 Egyptian epidemic.",

keywords = "Phlebovirus, Bunyaviridae, Mauritania, In vitro and in vivo, RVF, Virulence, Cytopathology",

author = "Anderson, {G. W.} and Saluzzo, {J. F.} and Ksiazek, {T. G.} and Smith, {J. F.} and W. Ennis and D. Thureen and Peters, {C. J.} and Digoutte, {J. P.}",

year = "1989",

doi = "10.1016/S0923-2516(89)80090-1",

language = "English (US)",

volume = "140",

pages = "129--138",

journal = "Research in Virology",

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AU - Anderson, G. W.

AU - Saluzzo, J. F.

AU - Ksiazek, T. G.

AU - Smith, J. F.

AU - Ennis, W.

AU - Thureen, D.

AU - Peters, C. J.

AU - Digoutte, J. P.

PY - 1989

Y1 - 1989

N2 - Several cell cultures and animals were compared for their relative sensitivity as primary isolation systems for Rift Valley fever virus (RVFV) and to determine if virulence characteristics of the isolates were altered in these systems. Eleven human sera from known cases of Rift Valley fever (RVF) were obtained from the 1987 epidemic in Mauritania and served as the source of virus for these studies. Sera were inoculated directly into cell cultures (Vero, C6/36 and DBS-FRhL-2) and animals (ICR suckling mice, Lak:LVG(SYR) hamsters and WF rats) concurrently. The cell lines provided a quick method to propagate, quantitate and identify these specimens without prior adaption. The isolates were highly virulent for suckling mice and hamsters, but not for WF rats, even after cell culture passage, which indicated that the Mauritanian isolates more closely resembled those strains from sub-Saharan Africa than those from the 1977-78 Egyptian epidemic.

AB - Several cell cultures and animals were compared for their relative sensitivity as primary isolation systems for Rift Valley fever virus (RVFV) and to determine if virulence characteristics of the isolates were altered in these systems. Eleven human sera from known cases of Rift Valley fever (RVF) were obtained from the 1987 epidemic in Mauritania and served as the source of virus for these studies. Sera were inoculated directly into cell cultures (Vero, C6/36 and DBS-FRhL-2) and animals (ICR suckling mice, Lak:LVG(SYR) hamsters and WF rats) concurrently. The cell lines provided a quick method to propagate, quantitate and identify these specimens without prior adaption. The isolates were highly virulent for suckling mice and hamsters, but not for WF rats, even after cell culture passage, which indicated that the Mauritanian isolates more closely resembled those strains from sub-Saharan Africa than those from the 1977-78 Egyptian epidemic.

KW - Phlebovirus, Bunyaviridae, Mauritania, In vitro and in vivo

KW - RVF, Virulence, Cytopathology

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JO - Research in Virology

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Comparison of in vitro and in vivo systems for propagation of rift valley fever virus from clinical specimens

Abstract

Keywords

ASJC Scopus subject areas

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