Comparison of longitudinal leukocyte gene expression after burn injury or trauma-hemorrhage in mice

James A. Lederer, Bernard H. Brownstein, M. Cecilia Lopez, Sandra MacMillan, Adam J. Delisle, Malcolm P. MacConmara, Mashkoor A. Choudhry, Wenzhong Xiao, Steven Lekousi, J. Perren Cobb, Henry V. Baker, John A. Mannick, Irshad H. Chaudry, Ulysses Balis, Paul Bankey, Timothy R. Billiar, Steven E. Calvano, David G. Camp, Joseph Cuschieri, Ronald W. DavisAsit K. De, Constance Elson, Celeste Finnerty, Bradley Freeman, Richard L. Gamelli, Nicole S. Gibran, Brian G. Harbrecht, Douglas L. Hayden, Laura Hennessy, David Herndon, Jureta W. Horton, Marc G. Jeschke, Jeffrey Johnson, Matthew B. Klein, Stephen F. Lowry, Ronald V. Maier, Philip H. Mason, Grace P. McDonald-Smith, Carol L. Miller-Graziano, Michael N. Mindrinos, Joseph P. Minei, Lyle L. Moldawer, Ernest E. Moore, Avery B. Nathens, Grant E. O'Keefe, Laurence G. Rahme, Daniel G. Remick, David A. Schoenfeld, Michael B. Shapiro, Geoffrey M. Silver, Richard D. Smith, John Storey, Robert Tibshirani, Ronald G. Tompkins, Mehmet Toner, H. Shaw Warren, Michael A. West

Research output: Contribution to journalArticle

19 Citations (Scopus)

Abstract

A primary objective of the large collaborative project entitled "Inflammation and the Host Response to Injury" was to identify leukocyte genes that are differentially expressed after two different types of injury in mouse models and to test the hypothesis that both forms of injury would induce similar changes in gene expression. We report here the genes that are expressed in white blood cells (WBCs) and in splenocytes at 2 h, 1 day, 3 days, and 7 days after burn and sham injury or trauma-hemorrhage (T-H) and sham T-H. Affymetrix Mouse Genome 430 2.0 GeneChips were used to profile gene expression, and the results were analyzed by dCHIP, BRB Array Tools, and Ingenuity Pathway Analysis (IPA) software. We found that the highest number of genes differentially expressed following burn injury were at day 1 for both WBCs (4,989) and for splenocytes (4,715) and at day 1 for WBCs (1,167) and at day 3 for splenocytes (1,117) following T-H. The maximum overlap of genes that were expressed after both forms of injury were at day 1 in WBCs (136 genes) and at day 7 in splenocytes (433 genes). IPA revealed that the cell-to-cell signaling, cell death, immune response, antiapoptosis, and cell cycle control pathways were affected most significantly. In summary, this report provides a database of genes that are modulated in WBCs and splenocytes at sequential time points after burn or T-H in mice and reveals that relatively few leukocyte genes are expressed in common after these two forms of injury.

Original languageEnglish
Pages (from-to)299-310
Number of pages12
JournalPhysiological Genomics
Volume32
Issue number3
DOIs
StatePublished - Feb 19 2008
Externally publishedYes

Fingerprint

Leukocytes
Hemorrhage
Gene Expression
Wounds and Injuries
Genes
Cell Cycle Checkpoints
Transcriptome
Cell Death
Software
Genome
Databases
Inflammation

Keywords

  • Gene microarray
  • Gene microarray analysis
  • Immune response
  • Inflammation
  • Pathway analysis

ASJC Scopus subject areas

  • Physiology
  • Genetics

Cite this

Lederer, J. A., Brownstein, B. H., Lopez, M. C., MacMillan, S., Delisle, A. J., MacConmara, M. P., ... West, M. A. (2008). Comparison of longitudinal leukocyte gene expression after burn injury or trauma-hemorrhage in mice. Physiological Genomics, 32(3), 299-310. https://doi.org/10.1152/physiolgenomics.00086.2007

Comparison of longitudinal leukocyte gene expression after burn injury or trauma-hemorrhage in mice. / Lederer, James A.; Brownstein, Bernard H.; Lopez, M. Cecilia; MacMillan, Sandra; Delisle, Adam J.; MacConmara, Malcolm P.; Choudhry, Mashkoor A.; Xiao, Wenzhong; Lekousi, Steven; Cobb, J. Perren; Baker, Henry V.; Mannick, John A.; Chaudry, Irshad H.; Balis, Ulysses; Bankey, Paul; Billiar, Timothy R.; Calvano, Steven E.; Camp, David G.; Cuschieri, Joseph; Davis, Ronald W.; De, Asit K.; Elson, Constance; Finnerty, Celeste; Freeman, Bradley; Gamelli, Richard L.; Gibran, Nicole S.; Harbrecht, Brian G.; Hayden, Douglas L.; Hennessy, Laura; Herndon, David; Horton, Jureta W.; Jeschke, Marc G.; Johnson, Jeffrey; Klein, Matthew B.; Lowry, Stephen F.; Maier, Ronald V.; Mason, Philip H.; McDonald-Smith, Grace P.; Miller-Graziano, Carol L.; Mindrinos, Michael N.; Minei, Joseph P.; Moldawer, Lyle L.; Moore, Ernest E.; Nathens, Avery B.; O'Keefe, Grant E.; Rahme, Laurence G.; Remick, Daniel G.; Schoenfeld, David A.; Shapiro, Michael B.; Silver, Geoffrey M.; Smith, Richard D.; Storey, John; Tibshirani, Robert; Tompkins, Ronald G.; Toner, Mehmet; Warren, H. Shaw; West, Michael A.

In: Physiological Genomics, Vol. 32, No. 3, 19.02.2008, p. 299-310.

Research output: Contribution to journalArticle

Lederer, JA, Brownstein, BH, Lopez, MC, MacMillan, S, Delisle, AJ, MacConmara, MP, Choudhry, MA, Xiao, W, Lekousi, S, Cobb, JP, Baker, HV, Mannick, JA, Chaudry, IH, Balis, U, Bankey, P, Billiar, TR, Calvano, SE, Camp, DG, Cuschieri, J, Davis, RW, De, AK, Elson, C, Finnerty, C, Freeman, B, Gamelli, RL, Gibran, NS, Harbrecht, BG, Hayden, DL, Hennessy, L, Herndon, D, Horton, JW, Jeschke, MG, Johnson, J, Klein, MB, Lowry, SF, Maier, RV, Mason, PH, McDonald-Smith, GP, Miller-Graziano, CL, Mindrinos, MN, Minei, JP, Moldawer, LL, Moore, EE, Nathens, AB, O'Keefe, GE, Rahme, LG, Remick, DG, Schoenfeld, DA, Shapiro, MB, Silver, GM, Smith, RD, Storey, J, Tibshirani, R, Tompkins, RG, Toner, M, Warren, HS & West, MA 2008, 'Comparison of longitudinal leukocyte gene expression after burn injury or trauma-hemorrhage in mice', Physiological Genomics, vol. 32, no. 3, pp. 299-310. https://doi.org/10.1152/physiolgenomics.00086.2007
Lederer JA, Brownstein BH, Lopez MC, MacMillan S, Delisle AJ, MacConmara MP et al. Comparison of longitudinal leukocyte gene expression after burn injury or trauma-hemorrhage in mice. Physiological Genomics. 2008 Feb 19;32(3):299-310. https://doi.org/10.1152/physiolgenomics.00086.2007
Lederer, James A. ; Brownstein, Bernard H. ; Lopez, M. Cecilia ; MacMillan, Sandra ; Delisle, Adam J. ; MacConmara, Malcolm P. ; Choudhry, Mashkoor A. ; Xiao, Wenzhong ; Lekousi, Steven ; Cobb, J. Perren ; Baker, Henry V. ; Mannick, John A. ; Chaudry, Irshad H. ; Balis, Ulysses ; Bankey, Paul ; Billiar, Timothy R. ; Calvano, Steven E. ; Camp, David G. ; Cuschieri, Joseph ; Davis, Ronald W. ; De, Asit K. ; Elson, Constance ; Finnerty, Celeste ; Freeman, Bradley ; Gamelli, Richard L. ; Gibran, Nicole S. ; Harbrecht, Brian G. ; Hayden, Douglas L. ; Hennessy, Laura ; Herndon, David ; Horton, Jureta W. ; Jeschke, Marc G. ; Johnson, Jeffrey ; Klein, Matthew B. ; Lowry, Stephen F. ; Maier, Ronald V. ; Mason, Philip H. ; McDonald-Smith, Grace P. ; Miller-Graziano, Carol L. ; Mindrinos, Michael N. ; Minei, Joseph P. ; Moldawer, Lyle L. ; Moore, Ernest E. ; Nathens, Avery B. ; O'Keefe, Grant E. ; Rahme, Laurence G. ; Remick, Daniel G. ; Schoenfeld, David A. ; Shapiro, Michael B. ; Silver, Geoffrey M. ; Smith, Richard D. ; Storey, John ; Tibshirani, Robert ; Tompkins, Ronald G. ; Toner, Mehmet ; Warren, H. Shaw ; West, Michael A. / Comparison of longitudinal leukocyte gene expression after burn injury or trauma-hemorrhage in mice. In: Physiological Genomics. 2008 ; Vol. 32, No. 3. pp. 299-310.
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AU - Brownstein, Bernard H.

AU - Lopez, M. Cecilia

AU - MacMillan, Sandra

AU - Delisle, Adam J.

AU - MacConmara, Malcolm P.

AU - Choudhry, Mashkoor A.

AU - Xiao, Wenzhong

AU - Lekousi, Steven

AU - Cobb, J. Perren

AU - Baker, Henry V.

AU - Mannick, John A.

AU - Chaudry, Irshad H.

AU - Balis, Ulysses

AU - Bankey, Paul

AU - Billiar, Timothy R.

AU - Calvano, Steven E.

AU - Camp, David G.

AU - Cuschieri, Joseph

AU - Davis, Ronald W.

AU - De, Asit K.

AU - Elson, Constance

AU - Finnerty, Celeste

AU - Freeman, Bradley

AU - Gamelli, Richard L.

AU - Gibran, Nicole S.

AU - Harbrecht, Brian G.

AU - Hayden, Douglas L.

AU - Hennessy, Laura

AU - Herndon, David

AU - Horton, Jureta W.

AU - Jeschke, Marc G.

AU - Johnson, Jeffrey

AU - Klein, Matthew B.

AU - Lowry, Stephen F.

AU - Maier, Ronald V.

AU - Mason, Philip H.

AU - McDonald-Smith, Grace P.

AU - Miller-Graziano, Carol L.

AU - Mindrinos, Michael N.

AU - Minei, Joseph P.

AU - Moldawer, Lyle L.

AU - Moore, Ernest E.

AU - Nathens, Avery B.

AU - O'Keefe, Grant E.

AU - Rahme, Laurence G.

AU - Remick, Daniel G.

AU - Schoenfeld, David A.

AU - Shapiro, Michael B.

AU - Silver, Geoffrey M.

AU - Smith, Richard D.

AU - Storey, John

AU - Tibshirani, Robert

AU - Tompkins, Ronald G.

AU - Toner, Mehmet

AU - Warren, H. Shaw

AU - West, Michael A.

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