Comparison of the genomes of the wild-type French viscerotropic strain of yellow fever virus with its vaccine derivative French neurotropic vaccine

E. Wang, K. D. Ryman, A. D. Jennings, D. J. Wood, F. Taffs, P. D. Minor, P. G. Sanders, Alan Barrett

Research output: Contribution to journalArticle

55 Citations (Scopus)

Abstract

The French neurotropic vaccine, or FNV, was used extensively in Africa to control yellow fever (YF). Although efficacious, the vaccine caused an unacceptable rate of post-vaccinal complications in children and was subsequently replaced by the 17D vaccine. Here we report that the genomes of the wild-type YF virus French viscerotropic virus and its attenuated vaccine derivative, FNV virus from the Institut Pasteur, Paris, (FNV-IP) differ by 77 nucleotides encoding 35 amino acid substitutions. Comparison of FNV-IP and three other isolates of FNV with other YF vaccine strains (17D-204 and 17DD derived from wild-type strain Asibi) revealed that during the two attenuation processes two common nucleotide changes arose that encode two amino acid substitutions: one is in the membrane protein at amino acid 35 (M-35), the other in non-structural (NS) protein 4B at NS4B-95. These common substitutions may be important in the process of attenuation of viscerotropic disease for humans and monkeys, and/or may be involved in loss of mosquito competence of the vaccine viruses.

Original languageEnglish (US)
Pages (from-to)2749-2755
Number of pages7
JournalJournal of General Virology
Volume76
Issue number11
StatePublished - 1995

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Yellow fever virus
Vaccines
Genome
Viruses
Paris
Amino Acid Substitution
Monkey Diseases
Nucleotides
Yellow Fever Vaccine
Yellow Fever
Attenuated Vaccines
Culicidae
Mental Competency
Membrane Proteins
Amino Acids
Proteins

ASJC Scopus subject areas

  • Immunology
  • Virology

Cite this

Comparison of the genomes of the wild-type French viscerotropic strain of yellow fever virus with its vaccine derivative French neurotropic vaccine. / Wang, E.; Ryman, K. D.; Jennings, A. D.; Wood, D. J.; Taffs, F.; Minor, P. D.; Sanders, P. G.; Barrett, Alan.

In: Journal of General Virology, Vol. 76, No. 11, 1995, p. 2749-2755.

Research output: Contribution to journalArticle

Wang, E. ; Ryman, K. D. ; Jennings, A. D. ; Wood, D. J. ; Taffs, F. ; Minor, P. D. ; Sanders, P. G. ; Barrett, Alan. / Comparison of the genomes of the wild-type French viscerotropic strain of yellow fever virus with its vaccine derivative French neurotropic vaccine. In: Journal of General Virology. 1995 ; Vol. 76, No. 11. pp. 2749-2755.
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