Comparison of the NMR solution structure and the x-ray crystal structure of rat metallothionein-2

Werner Braun, M. Vašák, A. H. Robbins, C. D. Stout, G. Wagner, J. H R Kägi, K. Wüthrich

Research output: Contribution to journalArticle

196 Citations (Scopus)

Abstract

Metallothioneins are small cysteine-rich proteins capable of binding heavy metal ions such as Zn2+ and Cd2+. They are ubiquitous tissue components in higher organisms, which tentatively have been attributed both unspecific protective functions against toxic metal ions and highly specific roles in fundamental zinc-regulated cellular processes. In this paper a detailed comparison of the NMR solution structure [Schultze, P., Wörgötter, E., Braun, W., Wagner, G., Vašák, M., Kägi, J. H. R. & Wüthrich, K. (1988) J. Mol. Biol. 203, 251-268] and a recent x-ray crystal structure [Robbins, A. H., McRee, D. E., Williamson, M., Collett, S. A., Xoung, N. H., Furey, W. F., Wang, B. C. & Stout, C. D. (1991) J. Mol. Biol. 221, 1269-1293] of rat metallothionein-2 shows that the metallothionein structures in crystals and in solution have identical molecular architectures. The structures obtained with both techniques now present a reliable basis for discussions on structure-function correlations in this class of metalloproteins.

Original languageEnglish (US)
Pages (from-to)10124-10128
Number of pages5
JournalProceedings of the National Academy of Sciences of the United States of America
Volume89
Issue number21
StatePublished - 1992
Externally publishedYes

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Metallothionein
X-Rays
Metalloproteins
Heavy Ions
Poisons
Heavy Metals
Protein Binding
Cysteine
Zinc
Metals
Ions

ASJC Scopus subject areas

  • Genetics
  • General

Cite this

Comparison of the NMR solution structure and the x-ray crystal structure of rat metallothionein-2. / Braun, Werner; Vašák, M.; Robbins, A. H.; Stout, C. D.; Wagner, G.; Kägi, J. H R; Wüthrich, K.

In: Proceedings of the National Academy of Sciences of the United States of America, Vol. 89, No. 21, 1992, p. 10124-10128.

Research output: Contribution to journalArticle

Braun, Werner ; Vašák, M. ; Robbins, A. H. ; Stout, C. D. ; Wagner, G. ; Kägi, J. H R ; Wüthrich, K. / Comparison of the NMR solution structure and the x-ray crystal structure of rat metallothionein-2. In: Proceedings of the National Academy of Sciences of the United States of America. 1992 ; Vol. 89, No. 21. pp. 10124-10128.
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