Comparison of the nucleotide and deduced amino acid sequences of the envelope protein genes of the wild-type french viscerotropic strain of yellow fever virus and the live vaccine strain, french neurotropic vaccine, derived from it

The envelope (E) protein genes of the wild-type yellow fever (YF) virus French viscerotropic virus and its’ vaccine derivative, French neurotropic virus (FNV), were compared and found to differ by 13 nucleotides that coded for 8 amino acid substitutions. Comparison of the E proteins of FNV and 17D, the vaccine strain derived from wild-type strain Asibi, showed that there was no common nucleotide change or amino acid substitution between these two vaccine strains. However, changes are clustered around amino acid positions 52-56 and may represent the common vaccine epitope shared by 17D and FNV vaccine viruses. The molecular basis of any difference in neurotropism and viscerotropism of YF virus, attributable to the E protein, remains unclear.