Comparison of transfusion with DCLHb or pRBCs for treatment of intraoperative anemia in sheep

Luiz A. Vane, J. Sean Funston, Robert Kirschner, Don Harper, Donald J. Deyo, Daniel L. Traber, Lillian L. Traber, George C. Kramer

Research output: Contribution to journalArticle

19 Scopus citations

Abstract

Isoflurane-anesthetized sheep were transfused with packed red blood cells (pRBCs) or diaspirin cross-linked hemoglobin (DCLHb) for treatment of intraoperative hemorrhage. A rapid 15-min hemorrhage with lactated Ringer (LR) infusion maintained filling pressure at baseline and reduced blood hemoglobin (Hb) to ∼5 g/dl. Sheep received 2 g/kg Hb, DCLHb (n = 6), or pRBCs (n = 7); control group received LR alone (n = 6). After 2 h, anesthesia was discontinued; sheep were monitored in the animal intensive care unit for 48 h. DCLHb expanded blood volume more, but increased total blood Hb less, than pRBCs. Lower Hb and increased methemoglobin resulted in lower arterial oxygen content compared with the pRBCs. DCLHb caused pulmonary hypertension (from 13 to 30 mmHg) and elevated filling pressure (from 6 to 15 mmHg). Cardiac outputs (CO) were similar for all groups during anesthesia; however, during recovery CO increased only in the LR and packed pRBCs groups. DCLHb may limit the reflex ability to increase CO after volume expansion. Hemodynamic effects of DCLHb may be exaggerated when infused after large-volume LR.

Original languageEnglish (US)
Pages (from-to)343-353
Number of pages11
JournalJournal of Applied Physiology
Volume92
Issue number1
DOIs
StatePublished - Jan 1 2002

Keywords

  • Diaspirin cross-linked hemoglobin
  • Fluid resuscitation
  • Hemoglobin
  • Hemorrhage
  • Packed red blood cells
  • Red blood cell substitutes
  • Shock

ASJC Scopus subject areas

  • Physiology
  • Physiology (medical)

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